Attention: World-2DPAGE is no longer maintained.

Sample Preparation and Post-separation Analysis

P99095:

ID   GLNA_STAAN; STANDARD; 2DG.
AC   P99095;
DT   31-MAR-2004, integrated into SWISS-2DPAGE (release 17).
DT   31-MAR-2004, 2D annotation version 1.
DT   19-MAY-2011, general annotation version 5.
DE   RecName: Full=Glutamine synthetase; Short=GS; EC=6.3.1.2; AltName:
DE   Full=Glutamate--ammonia ligase;.
GN   Name=glnA; OrderedLocusNames=SA1150;
OS   Staphylococcus aureus (strain N315).
OC   Bacteria; Firmicutes; Bacillales; Staphylococcus.
OX   NCBI_TaxID=158879;
MT   STAPHYLOCOCCUS.
IM   STAPHYLOCOCCUS.
RN   [1]
RP   MAPPING ON GEL.
RX   PubMed=15590099; DOI=10.1016/j.mimet.2004.09.017; [NCBI, Expasy, EBI, Israel, Japan]
RA   Scherl A., Francois P., Bento M., Deshusses J.M., Charbonnier Y.,
RA   Converset V., Huyghe A., Walter N., Hoogland C., Appel R.D., Sanchez
RA   J.-C., Zimmermann-Ivol C.G., Corthals G.L., Hochstrasser D.F., Schrenzel J.;
RT   ''''''Correlation of proteomic and transcriptomic profiles of
RT   Staphylococcus aureus during the post-exponential phase of growth'';'';'';
RL   J. Microbiol. Methods 60(2):247-257(2005).
CC   -!- SUBUNIT: Oligomer of 12 subunits arranged in the form of two hexagons
CC         (By similarity)
2D   -!- MASTER: STAPHYLOCOCCUS;
2D   -!-   PI/MW: SPOT 2D-001XV1=5.08/51050;
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001XV1: (R)KFAEEENVR(Y),13-21;
2D         (K)FAEEENVR(Y),14-21; (R)LQFTDILGTIK(N),25-35; (K)NVEVPVSQLEK(V),36-46;
2D         (K)TDGTPFEGDPR(A),101-111; (K)YADAVTACDNIQTFK(L),203-217;
2D         (K)ALGNHIYNQFINSK(S),410-423; (K)SIEWDYYR(T),424-431; (R)TQVSEWER(D),432-439.
2D   -!-   MAPPING: MALDI tandem mass spectrometry [1].
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DR   UniProtKB/Swiss-Prot; P99095; GLNA_STAAN.
//

The following 2-D map is available for this protein:

	Staphylococcus aureus

SWISS-2DPAGE (search AC)