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Sample Preparation and Post-separation Analysis
| Searching in 'SWISS-2DPAGE' for entry matching: P99152 |
SWISS-2DPAGE: P99152
P99152:
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ID EFTU_STAAN; STANDARD; 2DG.
AC P99152;
DT 31-MAR-2004, integrated into SWISS-2DPAGE (release 17).
DT 31-MAR-2004, 2D annotation version 1.
DT 19-MAY-2011, general annotation version 5.
DE RecName: Full=Elongation factor Tu; Short=EF-Tu;.
GN Name=tuf; Synonyms=tufA; OrderedLocusNames=SA0506;
OS Staphylococcus aureus (strain N315).
OC Bacteria; Firmicutes; Bacillales; Staphylococcus.
OX NCBI_TaxID=158879;
MT STAPHYLOCOCCUS.
IM STAPHYLOCOCCUS.
RN [1]
RP MAPPING ON GEL.
RX PubMed=15590099; DOI=10.1016/j.mimet.2004.09.017; [NCBI, Expasy, EBI, Israel, Japan]
RA Scherl A., Francois P., Bento M., Deshusses J.M., Charbonnier Y.,
RA Converset V., Huyghe A., Walter N., Hoogland C., Appel R.D., Sanchez
RA J.-C., Zimmermann-Ivol C.G., Corthals G.L., Hochstrasser D.F., Schrenzel J.;
RT ''''''Correlation of proteomic and transcriptomic profiles of
RT Staphylococcus aureus during the post-exponential phase of growth'';'';'';
RL J. Microbiol. Methods 60(2):247-257(2005).
CC -!- SUBUNIT: Monomer (By similarity)
2D -!- MASTER: STAPHYLOCOCCUS;
2D -!- PI/MW: SPOT 2D-001XVQ=4.85/43756;
2D -!- PI/MW: SPOT 2D-001XVZ=4.83/42906;
2D -!- PI/MW: SPOT 2D-001Y0Y=4.56/31224; !FRAGMENT!
2D -!- PI/MW: SPOT 2D-001Y3E=4.84/25871; !FRAGMENT!
2D -!- PI/MW: SPOT 2D-001Y5A=5.18/21457; !FRAGMENT!
2D -!- PEPTIDE MASSES: SPOT 2D-001Y0Y: 1045.5548; 1156.5582; 1252.5582;
2D 1369.8014; 1625.817; 1625.8171; 1703.8752; 1752.9075; 1831.9695;
2D 2005.989; 2006.9679; 2007.9569; 2202.9854; 2321.136; 3954.8875; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001XVQ: (R)GITINTSHIEYQTDKR(H),60-75;
2D (R)HYAHVDCPGHADYVK(N),76-90; (R)NVGVPALVVFLNK(V),125-137;
2D (R)DLLSEYDFPGDDVPVIAGSALK(A),156-177; (K)ILELMEAVDTYIPTPER(D),189-205;
2D (K)VGEEVEIIGLHDTSK(T),239-253; (K)TTVTGVEMFR(K),254-263;
2D (R)KLLDYAEAGDNIGALLR(G),264-280; (K)LLDYAEAGDNIGALLR(G),265-280; (R)GQVLAAPGSITPHTEFK(A),290-306.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001XVZ: (R)GITINTSHIEYQTDKR(H),60-75;
2D (R)HYAHVDCPGHADYVK(N),76-90; (R)NVGVPALVVFLNK(V),125-137;
2D (R)DLLSEYDFPGDDVPVIAGSALK(A),156-177; (K)ILELMEAVDTYIPTPER(D),189-205;
2D (K)VGEEVEIIGLHDTSK(T),239-253; (K)TTVTGVEMFR(K),254-263;
2D (K)LLDYAEAGDNIGALLR(G),265-280; (R)GQVLAAPGSITPHTEFK(A),290-306.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001Y0Y: (K)VGEEVEIIGLHDTSK(T),138-152.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001Y3E: (K)VGEEVEIIGLHDTSK(T),138-152; (K)LLDYAEAGDNIGALLR(G),164-179.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001Y5A: (K)VGEEVEIIGLHDTSK(T),239-253;
2D (R)KLLDYAEAGDNIGALLR(G),264-280; (K)LLDYAEAGDNIGALLR(G),265-280;
2D (R)GQVLAAPGSITPHTEFK(A),290-306; (R)HTPFFSNYRPQFYFR(T),320-334; (R)TVGSGVVTEIIK(-),383-394.
2D -!- MAPPING: MALDI tandem mass spectrometry [1]; SPOT 2D-001Y0Y:
2D Peptide mass fingerprinting [1].
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DR UniProtKB/Swiss-Prot; P99152; EFTU_STAAN.
//
The following 2-D map is available for this protein:
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Staphylococcus aureus
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SWISS-2DPAGE (search AC)
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