Attention: World-2DPAGE is no longer maintained.

Sample Preparation and Post-separation Analysis

TBA1B_HUMAN:

ID   TBA1B_HUMAN; STANDARD; 2DG.
AC   P68363; P05209;
DT   15-MAY-2003, integrated into SWISS-2DPAGE (release 16).
DT   31-MAR-2004, 2D annotation version 2.
DT   19-MAY-2011, general annotation version 6.
DE   RecName: Full=Tubulin alpha-1B chain; AltName: Full=Alpha-tubulin
DE   ubiquitous; AltName: Full=Tubulin K-alpha-1; AltName: Full=Tubulin
DE   alpha-ubiquitous chain;.
GN   Name=TUBA1B;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   LYMPHOCYTE_HUMAN, NUCLEOLI_HELA_1D_HUMAN.
IM   LYMPHOCYTE_HUMAN, NUCLEOLI_HELA_1D_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   PubMed=12429849; [NCBI, Expasy, EBI, Israel, Japan]
RA   Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C.,
RA   Greco A., Hochstrasser D.F., Diaz J.-J.;
RT   ''''''Functional proteomic analysis of human nucleolus'';'';'';
RL   Mol. Biol. Cell. 13(1):4100-4109(2002).
RN   [2]
RP   MAPPING ON GEL.
RA   Vuadens F., Crettaz D., Telenti A., Quadroni M., Duchosal M.A., Schneider
RA   P., Tissot J.-D.;
RT   ''''''New insights into HIV lymphocyte infection'';'';'';
RL   (IN) Sanchez J.-C., Corthals G.L., Hochstrasser D.F. (eds.);
RL   Biomedical Application of Proteomics, pp.245-262, Wiley-VCH, Weinheim (2004).
CC   -!- SUBUNIT: DIMER OF ALPHA AND BETA CHAINS 
1D   -!- MASTER: NUCLEOLI_HELA_1D_HUMAN;
1D   -!-   MW: BAND 1D-001V8H=56716;
1D   -!-   MW: BAND 1D-001V8I=55139;
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V8H: (R)AVFVDLEPTVIDEVR(T),65-79.
1D   -!-   PEPTIDE SEQUENCES: BAND 1D-001V8I: (R)AVFVDLEPTVIDEVR(T),65-79;
1D         (R)NLDIERPTYTNLNR(L),216-229; (K)VGINYQPPTVVPGGDLAK(V),353-370.
1D   -!-   MAPPING: Tandem mass spectrometry [1].
2D   -!- MASTER: LYMPHOCYTE_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001Y0I=5.06/43286;
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001Y0I:
2D         (K)TIGGGDDSFNTFFSETGAGK(H),41-60; (R)QLFHPEQLITGK(E),85-96;
2D         (R)LISQIVSSITASLR(F),230-243; (R)IHFPLATYAPVISAEK(A),265-280;
2D         (R)SIQFVDWCPTGFK(V),340-352; (K)VGINYQPPTVVPGGDLAK(V),353-370.
2D   -!-   EXPRESSION: expressed predominantly in CD8 and CD4 lymphocytes [2].
2D   -!-   MAPPING: MALDI tandem mass spectrometry [2].
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DR   HSC-2DPAGE; P04687; HUMAN.
DR   OGP-WWW; P05209.
DR   UCD-2DPAGE; P68363; TBA1B_HUMAN.
DR   UniProtKB/Swiss-Prot; P68363; TBA1B_HUMAN.
//

The following 2-D maps are available for this protein:

	Lymphocytes	SDS-PAGE of nucleolar proteins from Human HeLa cells

SWISS-2DPAGE (search AC)