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] has enabled the identification and characterization of proteins from complex biological samples. Transfer of the proteins can be carried out using several methods such as vacuum, capillary or electric field. Electroblotting is by far the most wide-spread technique which utilizes either
. Both techniques can use either the Towbin or 3-[cyclohexamino]-1-propanesulfonic acid (CAPS) transfer buffers, depending on the need for minimal glycine contamination in post-transfer protein characterization. These two buffer systems are described here: