Attention: World-2DPAGE is no longer maintained.

SWISS-2DPAGE  Search by  [accession number]  [description, ID or gene]  [author names]  [spot ID / serial number]  [identification methods]  [pI / Mw range]  [combined fields]  Maps  [experimental info]  [protein list]  [graphical interface]      Select Remote Interfaces [All Interfaces] World-2DPAGE Portal World-2DPAGE Repository Exclude local DBs has only effect if a remote interface is selected

Sample Preparation and Post-separation Analysis Searching in 'SWISS-2DPAGE' for entry matching: P23301 SWISS-2DPAGE:  P23301 P23301 General information about the entry View entry in simple text format Entry name IF5A1_YEAST Primary accession number P23301 integrated into SWISS-2DPAGE on  February 1, 1995 (release 1) 2D Annotations were last modified on May 15, 2003 (version 1) General Annotations were last modified on  May 19, 2011 (version 13) Name and origin of the protein Description RecName: Full=Eukaryotic translation initiation factor 5A-1; Short=eIF-5A-1; AltName: Full=Hypusine-containing protein HP2; AltName: Full=eIF-4D;. Gene name Name=HYP2 Synonyms=TIF51A OrderedLocusNames=YEL034W ORFNames=SYGP-ORF21 Annotated species Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. References [1]   MAPPING ON GEL MEDLINE=96314060; PubMed=8740180; [NCBI, Expasy, EBI, Israel, Japan] Sanchez J.-C., Golaz O., Frutiger S., Schaller D., Appel R.D., Bairoch A., Hughes G.J., Hochstrasser D.F. ''''''The yeast SWISS-2DPAGE database'';'';'' Electrophoresis 17(1):556-565(1996) [2]   MAPPING ON GEL MEDLINE=93092944; PubMed=1281091; [NCBI, Expasy, EBI, Israel, Japan] Klier H., Lottspeich F. ''''''Detection of the hypusine-containing protein (HP = eIF-5A) in crude yeast extracts by two-dimensional western blots'';'';'' Electrophoresis 13(1):732-735(1992) Comments SUBUNIT: MONOMER 2D PAGE maps for identified proteins How to interpret a protein YEAST {Saccharomyces cerevisiae} Saccharomyces cerevisiae (Baker's yeast)   map experimental info   protein estimated location   YEAST MAP LOCATIONS: SPOT 2D-000JWS: pI=4.76; Mw=15388 SPOT 2D-000JWT: pI=4.66; Mw=15434 MAPPING (identification): AMINO ACID COMPOSITION ANALYSIS [1] AND IDENTIFIED ON 2D GELS BY KLIER AND LOTTSPEICH [2]. Copyright This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss). Cross-references UniProtKB/Swiss-Prot P23301; IF5A1_YEAST. YEPD 9236. YEPD 9248. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_10 Entry name IF5A1_YEAST Primary accession number P23301 Secondary accession number(s) D3DLL5 Sequence was last modified on  January 23, 2007 (version 3) Annotations were last modified on  September 21, 2011 (version 116) Name and origin of the protein Description RecName: Full=Eukaryotic translation initiation factor 5A-1; Short=eIF-5A-1; AltName: Full=Hypusine-containing protein HP2; AltName: Full=eIF-4D; Gene name Name=HYP2 Synonyms=TIF51A OrderedLocusNames=YEL034W ORFNames=SYGP-ORF21 Encoded on Name=HYP2; Synonyms=TIF51A; OrderedLocusNames=YEL034W; ORFNames=SYGP-ORF21 Keywords 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Elongation factor; Hypusine; Phosphoprotein; Protein biosynthesis; Reference proteome; RNA-binding. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL M63541; AAA35155.1; -; Genomic_DNA EMBL X56236; CAA39693.1; -; Genomic_DNA EMBL D83166; BAA11826.1; -; mRNA EMBL U18779; AAB65008.1; -; Genomic_DNA EMBL BK006939; DAA07619.1; -; Genomic_DNA PIR A40259; FIBYA1; . RefSeq NP_010880.1; NM_001178849.1; . PDB 3ER0; X-ray; 3.35 A; A/B=1-157 PDBsum 3ER0; -; . ProteinModelPortal P23301; -; . SMR P23301; 12-157; . DIP DIP-4230N; -; . IntAct P23301; 17; . MINT MINT-545311; -; . STRING P23301; -; . SWISS-2DPAGE P23301; -; . PeptideAtlas P23301; -; . EnsemblFungi YEL034W; YEL034W; YEL034W; . GeneID 856677; -; . KEGG sce:YEL034W; -; . NMPDR fig|4932.3.peg.1932; -; . SGD S000000760; HYP2; . eggNOG fuNOG08157; -; . GeneTree EFGT00050000003235; -; . HOGENOM HBG526951; -; . OrthoDB EOG4B01ZQ; -; . PhylomeDB P23301; -; . NextBio 982702; -; . ArrayExpress P23301; -; . Genevestigator P23301; -; . GermOnline YEL034W; Saccharomyces cerevisiae; . GO GO:0022626; C:cytosolic ribosome; IPI:SGD; . GO GO:0005739; C:mitochondrion; IDA:SGD; . GO GO:0043022; F:ribosome binding; IPI:SGD; . GO GO:0003746; F:translation elongation factor activity; IDA:SGD; . GO GO:0003743; F:translation initiation factor activity; IDA:SGD; . GO GO:0008612; P:peptidyl-lysine modification to hypusine; IEA:InterPro; . GO GO:0045901; P:positive regulation of translational elongation; IDA:SGD; . GO GO:0045948; P:positive regulation of translational initiation; IDA:SGD; . GO GO:0045905; P:positive regulation of translational termination; IDA:SGD; . GO GO:0006452; P:translational frameshifting; IMP:SGD; . InterPro IPR005824; KOW; . InterPro IPR012340; NA-bd_OB-fold; . InterPro IPR016027; NA-bd_OB-fold-like; . InterPro IPR019769; Trans_elong_IF5A_hypusine_site; . InterPro IPR001884; Transl_elong_IF5A; . InterPro IPR020189; Transl_elong_IF5A_C; . InterPro IPR014722; Transl_SH3-like_sub; . InterPro IPR008991; Translation_prot_SH3-like; . Gene3D G3DSA:2.40.50.140; OB_NA_bd_sub; 1; . Gene3D G3DSA:2.30.30.30; Ribosomal_L2; 1; . PANTHER PTHR11673; EIF5A_hypusine; 1; . Pfam PF01287; eIF-5a; 1; . Pfam PF00467; KOW; 1; . PIRSF PIRSF003025; eIF5A; 1; . SUPFAM SSF50249; Nucleic_acid_OB; 1; . SUPFAM SSF50104; Transl_SH3_like; 1; . TIGRFAMs TIGR00037; EIF_5A; 1; . PROSITE PS00302; IF5A_HYPUSINE; 1; . SWISS-2DPAGE (search AC)