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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: LIVK_ECOLI




SWISS-2DPAGE:  LIVK_ECOLI


LIVK_ECOLI


General information about the entry
View entry in simple text format
Entry nameLIVK_ECOLI
Primary accession numberP04816
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMay 15, 2003 (version 4)
General Annotations were last modified on May 19, 2011 (version 13)
Name and origin of the protein
DescriptionRecName: Full=Leucine-specific-binding protein; Short=L-BP; Short=LS-BP; Flags: Precursor;.
Gene nameName=livK
OrderedLocusNames=b3458, JW3423
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
MEDLINE=83270974; PubMed=6308410; [NCBI, ExPASy, EBI, Israel, Japan]
Neidhardt F.C., Vaughn V., Phillips T.A., Bloch P.L.
''''''Gene-protein index of Escherichia coli K-12'';'';''
Microbiol. Rev. 47(1):231-284(1983)
[3]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[4]   PROTEIN EXPRESSION
MEDLINE=94042879; PubMed=8226654; [NCBI, ExPASy, EBI, Israel, Japan]
Gage D.J., Neidhardt F.C.
''''''Adaptation of Escherichia coli to the uncoupler of oxidative phosphorylation 2,4-dinitrophenol'';'';''
J. Bacteriol. 175(1):7105-7108(1993)
[5]   PROTEIN EXPRESSION
MEDLINE=93352420; PubMed=8349554; [NCBI, ExPASy, EBI, Israel, Japan]
Sankar P., Hutton M.E., Vanbogelen R.A., Clark R.L., Neidhardt F.C.
''''''Expression analysis of cloned chromosomal segments of Escherichia coli'';'';''
J. Bacteriol. 175(1):5145-5152(1993)
[6]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[7]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=5.00; Mw=41546

EXPRESSION:
INCREASED FOLLOWING EXPOSURE TO DNP [4]; WAS ENCODED AND EXPRESSED FROM PLASMID PFNK613L [5].

MAPPING (identification):
AMINO ACID COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY NEIDHARDT [2] AND VANBOGELEN [3].



ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=5.08; Mw=39816  [identification data]
pI=5.56; Mw=29053  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [6];
SPOT 2D-00152C: MICROSEQUENCING (DDIK) [6].



ECOLI5-6 {Escherichia coli(5-6)}
Escherichia coli
ECOLI5-6
  map experimental info
  protein estimated location
 
ECOLI5-6

MAP LOCATIONS:
pI=5.25; Mw=59333  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [6].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=5.09; Mw=39439  [identification data]

EXPRESSION:
decrease after benzoic acid treatment [7].

MAPPING (identification):
Peptide mass fingerprinting and tandem mass spectrometry [7].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
2DBase-EcoliP04816; LIVK_ECOLI.
ECO2DBASEB040.8; 6TH EDITION.
UniProtKB/Swiss-ProtP04816; LIVK_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameLIVK_ECOLI
Primary accession numberP04816
Secondary accession number(s) Q2M7C1 Q47343
Sequence was last modified on July 19, 2003 (version 2)
Annotations were last modified on October 19, 2011 (version 107)
Name and origin of the protein
DescriptionRecName: Full=Leucine-specific-binding protein; Short=L-BP; Short=LS-BP; Flags: Precursor;
Gene nameName=livK
OrderedLocusNames=b3458, JW3423
Encoded onName=livK; OrderedLocusNames=b3458, JW3423
Keywords3D-structure; Amino-acid transport; Complete proteome; Direct protein sequencing; Disulfide bond; Periplasm; Reference proteome; Signal; Transport.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM29378; AAA24090.1; -; Genomic_DNA
EMBLJ05516; AAA83883.1; -; Genomic_DNA
EMBLU00039; AAB18433.1; -; Genomic_DNA
EMBLU00096; AAC76483.1; -; Genomic_DNA
EMBLAP009048; BAE77835.1; -; Genomic_DNA
EMBLV00301; CAA23579.1; -; Genomic_DNA
PIRE65142; BLECL; .
RefSeqNP_417915.1; NC_000913.2; .
PDB1USG; X-ray; 1.53 A; A=24-369
PDB1USI; X-ray; 1.80 A; A/C=24-369
PDB1USK; X-ray; 2.00 A; A/B/C/D=24-369
PDB2LBP; X-ray; 2.40 A; A=24-369
PDBsum1USG; -; .
PDBsum1USI; -; .
PDBsum1USK; -; .
PDBsum2LBP; -; .
ProteinModelPortalP04816; -; .
SMRP04816; 24-368; .
IntActP04816; 3; .
TCDB3.A.1.4.1; ATP-binding cassette (ABC) superfamily; .
SWISS-2DPAGEP04816; -; .
2DBase-EcoliP04816; -; .
ECO2DBASEB040.8; 6TH EDITION; .
EnsemblBacteriaEBESCT00000003254; EBESCP00000003254; EBESCG00000002670; .
EnsemblBacteriaEBESCT00000003255; EBESCP00000003255; EBESCG00000002670; .
EnsemblBacteriaEBESCT00000014562; EBESCP00000013853; EBESCG00000013623; .
GeneID947964; -; .
GenomeReviewsAP009048_GR; JW3423; .
GenomeReviewsU00096_GR; b3458; .
KEGGecj:JW3423; -; .
KEGGeco:b3458; -; .
EchoBASEEB0535; -; .
EcoGeneEG10540; livK; .
eggNOGCOG0683; -; .
GeneTreeEBGT00050000010005; -; .
HOGENOMHBG695960; -; .
OMAMEFTGAR; -; .
ProtClustDBPRK15404; -; .
BioCycEcoCyc:LIVK-MONOMER; -; .
GenevestigatorP04816; -; .
GOGO:0030288; C:outer membrane-bounded periplasmic space; IEA:InterPro; .
GOGO:0006865; P:amino acid transport; IEA:UniProtKB-KW; .
InterProIPR001828; ANF_lig-bd_rcpt; .
InterProIPR000709; Leu_Ile_Val-bd; .
PfamPF01094; ANF_receptor; 1; .
PRINTSPR00337; LEUILEVALBP; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server