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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P0A6F5




SWISS-2DPAGE:  P0A6F5


P0A6F5


General information about the entry
View entry in simple text format
Entry nameCH60_ECOLI
Primary accession numberP0A6F5
Secondary accession number(s) P06139
integrated into SWISS-2DPAGE on August 1, 1995 (release 2)
2D Annotations were last modified onMarch 31, 2004 (version 5)
General Annotations were last modified on May 19, 2011 (version 14)
Name and origin of the protein
DescriptionRecName: Full=60 kDa chaperonin; AltName: Full=GroEL protein; AltName: Full=Protein Cpn60;.
Gene nameName=groL
Synonyms=groEL, mopA
OrderedLocusNames=b4143, JW4103
Annotated speciesEscherichia coli [TaxID: 562]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL
MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F.
''''''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'';'';''
Electrophoresis 17(1):547-555(1996)
[2]   MAPPING ON GEL
Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.
''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';''
(IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press, Washington DC (1996)
[3]   PROTEIN EXPRESSION
MEDLINE=94042879; PubMed=8226654; [NCBI, ExPASy, EBI, Israel, Japan]
Gage D.J., Neidhardt F.C.
''''''Adaptation of Escherichia coli to the uncoupler of oxidative phosphorylation 2,4-dinitrophenol'';'';''
J. Bacteriol. 175(1):7105-7108(1993)
[4]   PROTEIN EXPRESSION
MEDLINE=87083376; PubMed=3539918; [NCBI, ExPASy, EBI, Israel, Japan]
Vanbogelen R.A., Kelley P.M., Neidhardt F.C.
''''''Differential induction of heat shock, SOS, and oxidation stress regulons and accumulation of nucleotides in Escherichia coli'';'';''
J. Bacteriol. 169(1):26-32(1987)
[5]   MAPPING ON GEL
PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C.
''''''New perspectives in the Escherichia coli proteome investigation'';'';''
Proteomics 1(1):409-423(2001)
[6]   MAPPING ON GEL
PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.
''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';''
Proteomics 2(1):1682-1698(2002)
Comments
  • SUBUNIT: OLIGOMER OF 14 SUBUNITS COMPOSED OF TWO STACKED RINGS OF 7 SUBUNITS
2D PAGE maps for identified proteins
How to interpret a protein

ECOLI {Escherichia coli}
Escherichia coli
ECOLI
  map experimental info
  protein estimated location
 
ECOLI

MAP LOCATIONS:
pI=4.85; Mw=56743

EXPRESSION:
INCREASED FOLLOWING EXPOSURE TO DNP [3]; WAS INDUCED BY TEMPERATURE SHIFTS, ETHANOL, NALIDIXIC ACID AND PUROMYCIN [4].

MAPPING (identification):
AMINO ACID COMPOSITION AND MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].



ECOLI4-5 {Escherichia coli(4-5)}
Escherichia coli
ECOLI4-5
  map experimental info
  protein estimated location
 
ECOLI4-5

MAP LOCATIONS:
pI=4.97; Mw=61125  [identification data]
pI=4.99; Mw=56877  [identification data]
pI=4.93; Mw=55185  [identification data]
pI=4.95; Mw=55185  [identification data]
pI=4.95; Mw=55185  [identification data]
pI=4.84; Mw=52802  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [5]; SPOTS 1HGK, 1HGU: GEL MATCHING WITH MASTER ECOLI4.5-5.5 [5].



ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)}
Escherichia coli
ECOLI4.5-5.5
  map experimental info
  protein estimated location
 
ECOLI4.5-5.5

MAP LOCATIONS:
pI=4.99; Mw=55445  [identification data]
pI=4.96; Mw=54287  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [5];
SPOT 2D-0014V3: MICROSEQUENCING (XLRG) [5].



ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)}
Escherichia coli
ECOLI-DIGE4.5-6.5
  map experimental info
  protein estimated location
 
ECOLI-DIGE4.5-6.5

MAP LOCATIONS:
pI=4.93; Mw=61278  [identification data]
pI=4.96; Mw=59987  [identification data]
pI=4.98; Mw=60758  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [6];
SPOT 2D-001WFW: TANDEM MASS SPECTROMETRY [6].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
2DBase-EcoliP0A6F5; CH60_ECOLI.
ECO2DBASEB056.5; 6TH EDITION.
UniProtKB/Swiss-ProtP0A6F5; CH60_ECOLI.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameCH60_ECOLI
Primary accession numberP0A6F5
Secondary accession number(s) P06139 Q2M6G1
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 79)
Name and origin of the protein
DescriptionRecName: Full=60 kDa chaperonin; AltName: Full=GroEL protein; AltName: Full=Protein Cpn60;
Gene nameName=groL
Synonyms=groEL, mopA
OrderedLocusNames=b4143, JW4103
Encoded onName=groL; Synonyms=groEL, mopA; OrderedLocusNames=b4143, JW4103
Keywords3D-structure; Acetylation; ATP-binding; Cell cycle; Cell division; Chaperone; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleotide-binding; Phosphoprotein; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX07850; CAA30698.1; -; Genomic_DNA
EMBLU14003; AAA97042.1; -; Genomic_DNA
EMBLU00096; AAC77103.1; -; Genomic_DNA
EMBLAP009048; BAE78145.1; -; Genomic_DNA
EMBLX07899; CAA30739.1; -; Genomic_DNA
EMBLM11294; AAA23934.1; ALT_FRAME; Genomic_DNA
PIRS56371; BVECGL; .
RefSeqNP_418567.1; NC_000913.2; .
PDB1AON; X-ray; 3.00 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-548
PDB1DK7; X-ray; 2.02 A; A/B=191-336
PDB1DKD; X-ray; 2.10 A; A/B/C/D=191-336
PDB1FY9; X-ray; 2.20 A; A=191-376
PDB1FYA; X-ray; 2.20 A; A=191-376
PDB1GR5; EM; 7.90 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-548
PDB1GRL; X-ray; 2.80 A; A/B/C/D/E/F/G=1-548
PDB1GRU; EM; 12.50 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-548
PDB1J4Z; X-ray; 3.52 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-547
PDB1JON; X-ray; 2.50 A; A=191-345
PDB1KID; X-ray; 1.70 A; A=191-375
PDB1KP8; X-ray; 2.00 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-547
PDB1KPO; X-ray; 3.52 A; 1/2/O/P/Q/R/S/T/U/V/W/X/Y/Z=2-547
PDB1LA1; X-ray; 2.06 A; A=188-379
PDB1MNF; X-ray; 3.00 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-547
PDB1OEL; X-ray; 2.80 A; A/B/C/D/E/F/G=2-548
PDB1PCQ; X-ray; 2.81 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-524
PDB1PF9; X-ray; 2.99 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=1-525
PDB1SS8; X-ray; 2.70 A; A/B/C/D/E/F/G=2-524
PDB1SVT; X-ray; 2.81 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-524
PDB1SX3; X-ray; 2.00 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-525
PDB1SX4; X-ray; 3.00 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-524
PDB1XCK; X-ray; 2.92 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=1-548
PDB2C7C; EM; 7.70 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-547
PDB2C7D; EM; 8.70 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-547
PDB2C7E; EM; 9.70 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-548
PDB2CGT; EM; 8.20 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-547
PDB2EU1; X-ray; 3.29 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=1-548
PDB2NWC; X-ray; 3.02 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-548
PDB2YEY; X-ray; 3.30 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-525
PDB3C9V; EM; 4.70 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-527
PDB3CAU; EM; 4.20 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=2-527
PDBsum1AON; -; .
PDBsum1DK7; -; .
PDBsum1DKD; -; .
PDBsum1FY9; -; .
PDBsum1FYA; -; .
PDBsum1GR5; -; .
PDBsum1GRL; -; .
PDBsum1GRU; -; .
PDBsum1J4Z; -; .
PDBsum1JON; -; .
PDBsum1KID; -; .
PDBsum1KP8; -; .
PDBsum1KPO; -; .
PDBsum1LA1; -; .
PDBsum1MNF; -; .
PDBsum1OEL; -; .
PDBsum1PCQ; -; .
PDBsum1PF9; -; .
PDBsum1SS8; -; .
PDBsum1SVT; -; .
PDBsum1SX3; -; .
PDBsum1SX4; -; .
PDBsum1XCK; -; .
PDBsum2C7C; -; .
PDBsum2C7D; -; .
PDBsum2C7E; -; .
PDBsum2CGT; -; .
PDBsum2EU1; -; .
PDBsum2NWC; -; .
PDBsum2YEY; -; .
PDBsum3C9V; -; .
PDBsum3CAU; -; .
ProteinModelPortalP0A6F5; -; .
SMRP0A6F5; 2-525; .
DIPDIP-339N; -; .
IntActP0A6F5; 690; .
MINTMINT-5232496; -; .
SWISS-2DPAGEP0A6F5; -; .
2DBase-EcoliP0A6F5; -; .
ECO2DBASEB056.5; 6TH EDITION; .
PRIDEP0A6F5; -; .
EnsemblBacteriaEBESCT00000003358; EBESCP00000003358; EBESCG00000002749; .
EnsemblBacteriaEBESCT00000017770; EBESCP00000017061; EBESCG00000016826; .
GeneID948665; -; .
GenomeReviewsAP009048_GR; JW4103; .
GenomeReviewsU00096_GR; b4143; .
KEGGecj:JW4103; -; .
KEGGeco:b4143; -; .
EchoBASEEB0594; -; .
EcoGeneEG10599; groL; .
eggNOGCOG0459; -; .
GeneTreeEBGT00050000009756; -; .
HOGENOMHBG625289; -; .
OMADKPAAGM; -; .
ProtClustDBPRK00013; -; .
BioCycEcoCyc:EG10599-MONOMER; -; .
GenevestigatorP0A6F5; -; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0016020; C:membrane; IDA:UniProtKB; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0007049; P:cell cycle; IEA:UniProtKB-KW; .
GOGO:0051301; P:cell division; IEA:UniProtKB-KW; .
GOGO:0042026; P:protein refolding; IEA:InterPro; .
HAMAPMF_00600; CH60; 1; -
InterProIPR018370; Chaperonin_Cpn60_CS; .
InterProIPR001844; Chaprnin_Cpn60; .
InterProIPR002423; Cpn60/TCP-1; .
PANTHERPTHR11353; Cpn60/TCP-1; 1; .
PfamPF00118; Cpn60_TCP1; 1; .
PRINTSPR00298; CHAPERONIN60; .
SUPFAMSSF48592; GroEL-ATPase; 1; .
TIGRFAMsTIGR02348; GroEL; 1; .
PROSITEPS00296; CHAPERONINS_CPN60; 1; .



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SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server