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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P62937




SWISS-2DPAGE:  P62937


P62937


General information about the entry
View entry in simple text format
Entry namePPIA_HUMAN
Primary accession numberP62937
Secondary accession number(s) P05092
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onMarch 31, 2004 (version 4)
General Annotations were last modified on May 19, 2011 (version 12)
Name and origin of the protein
DescriptionRecName: Full=Peptidyl-prolyl cis-trans isomerase A; Short=PPIase A; EC=5.2.1.8; AltName: Full=Cyclophilin A; AltName: Full=Cyclosporin A-binding protein; AltName: Full=Rotamase A;.
Gene nameName=PPIA
Synonyms=CYPA
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=93162045; PubMed=1286669; [NCBI, ExPASy, EBI, Israel, Japan]
Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F., Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel R.D., Hughes G.J.
''''''Human liver protein map: a reference database established by microsequencing and gel comparison'';'';''
Electrophoresis 13(1):992-1001(1992)
[2]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
[3]   MAPPING ON GEL
Tonella L., Hoogland C., Appel R.D., Sanchez J.-C., Hochstrasser D.F.
Submitted (JUL-1997) to SWISS-2DPAGE
[4]   MAPPING ON GEL
MEDLINE=96314065; PubMed=8740185; [NCBI, ExPASy, EBI, Israel, Japan]
Lutomski D., Caron M., Cornillot J.D., Bourin P., Dupuy C., Pontet M., Bladier D., Joubert-Caron R.
''''''Identification of different galectins by immunoblotting after two- dimensional polyacrylamide get electrophoresis with immobilized pH gradients'';'';''
Electrophoresis 17(1):600-606(1996)
[5]   MAPPING ON GEL
Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A., Appel R.D., Hochstrasser D.F.
Submitted (JAN-1999) to SWISS-2DPAGE
[6]   MAPPING ON GEL
Vuadens F., Crettaz D., Telenti A., Quadroni M., Duchosal M.A., Schneider P., Tissot J.-D.
''''''New insights into HIV lymphocyte infection'';'';''
(IN) Sanchez J.-C., Corthals G.L., Hochstrasser D.F. (eds.)Biomedical Application of Proteomics, pp.245-262, Wiley-VCH, Weinheim (2004)
Comments
  • SUBUNIT: SINGLE CHAIN
2D PAGE maps for identified proteins
How to interpret a protein

DLD1_HUMAN {Colorectal adenocarcinoma cell line (DL-1)}
Homo sapiens (Human)
Tissue: Colon adenocarcinoma
DLD1_HUMAN
  map experimental info
  protein estimated location
 
DLD1_HUMAN

MAP LOCATIONS:
pI=7.06; Mw=14905  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [5].



ELC_HUMAN {Erythroleukemia Cell}
Homo sapiens (Human)
Tissue: Erythroleukemia
ELC_HUMAN
  map experimental info
  protein estimated location
 
ELC_HUMAN

MAP LOCATIONS:
pI=6.96; Mw=14543
pI=7.22; Mw=14500
pI=6.79; Mw=14357
pI=8.11; Mw=13730

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



HL60_HUMAN {Promyelocytic leukemia cells}
Homo sapiens (Human)
Tissue: Promyelocytic leukemia
HL60_HUMAN
  map experimental info
  protein estimated location
 
HL60_HUMAN

MAP LOCATIONS:
pI=6.70; Mw=13227
pI=6.44; Mw=13193
pI=7.08; Mw=13159

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [3][4].



LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=6.69; Mw=14545
pI=7.07; Mw=14514
pI=7.60; Mw=14514
pI=8.03; Mw=14514

MAPPING (identification):
MICROSEQUENCING [1].



LYMPHOCYTE_HUMAN {Lymphocytes}
Homo sapiens (Human)
Tissue: Lymphocyte
LYMPHOCYTE_HUMAN
  map experimental info
  protein estimated location
 
LYMPHOCYTE_HUMAN

MAP LOCATIONS:
pI=7.82; Mw=17900  [identification data]
pI=7.31; Mw=17780  [identification data]

MAPPING (identification):
Peptide mass fingerprinting [6].



LYMPHOMA_HUMAN {Lymphoma}
Homo sapiens (Human)
Tissue: Lymphoma
LYMPHOMA_HUMAN
  map experimental info
  protein estimated location
 
LYMPHOMA_HUMAN

MAP LOCATIONS:
pI=7.11; Mw=14927
pI=7.54; Mw=14849

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



U937_HUMAN {Macrophage Like Cell Line}
Homo sapiens (Human)
Tissue: Histiocytic lymphoma
U937_HUMAN
  map experimental info
  protein estimated location
 
U937_HUMAN

MAP LOCATIONS:
pI=7.15; Mw=13972
pI=7.44; Mw=13808

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
DOSAC-COBS 2D-PAGEP62937; P62937.
DOSAC-COBS-2DPAGEP62937; PPIA_HUMAN.
OGP-WWWP05092.
PMMA-2DPAGEP05092; PPIA_HUMAN.
REPRODUCTION-2DPAGEP62937; P62937.
REPRODUCTION-2DPAGEIPI00419585; IPI00419585.
Siena-2DPAGEP05092; PPIA_HUMAN.
UCD-2DPAGEP62937; PPIA_HUMAN.
UniProtKB/Swiss-ProtP62937; PPIA_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry namePPIA_HUMAN
Primary accession numberP62937
Secondary accession number(s) P05092 Q3KQW3 Q6IBU5 Q96IX3 Q9BRU4 Q9BTY9 Q9UC61
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on October 19, 2011 (version 93)
Name and origin of the protein
DescriptionRecName: Full=Peptidyl-prolyl cis-trans isomerase A; Short=PPIase A; EC=5.2.1.8; AltName: Full=Cyclophilin A; AltName: Full=Cyclosporin A-binding protein; AltName: Full=Rotamase A;
Gene nameName=PPIA
Synonyms=CYPA
Encoded onName=PPIA; Synonyms=CYPA
Keywords3D-structure; Acetylation; Complete proteome; Cyclosporin; Cytoplasm; Direct protein sequencing; Host-virus interaction; Isomerase; Isopeptide bond; Phosphoprotein; Reference proteome; Rotamase; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLY00052; CAA68264.1; -; mRNA
EMBLX52851; CAA37039.1; -; Genomic_DNA
EMBLCR456707; CAG32988.1; -; mRNA
EMBLAY739283; AAU13906.1; -; Genomic_DNA
EMBLBC000689; AAH00689.1; -; mRNA
EMBLBC003026; AAH03026.2; -; mRNA
EMBLBC005320; AAH05320.1; -; mRNA
EMBLBC005982; AAH05982.1; -; mRNA
EMBLBC007104; AAH07104.1; -; mRNA
EMBLBC013915; AAH13915.1; -; mRNA
EMBLBC073992; AAH73992.1; -; mRNA
EMBLBC106030; AAI06031.1; -; mRNA
IPIIPI00419585; -; .
PIRA94496; CSHUA; .
RefSeqNP_066953.1; NM_021130.3; .
UniGeneHs.356331; -; .
UniGeneHs.598115; -; .
PDB1AK4; X-ray; 2.36 A; A/B=2-164
PDB1AWQ; X-ray; 1.58 A; A=2-165
PDB1AWR; X-ray; 1.58 A; A/B/C/D/E/F=2-165
PDB1AWS; X-ray; 2.55 A; A=2-165
PDB1AWT; X-ray; 2.55 A; A/B/C/D/E/F=2-165
PDB1AWU; X-ray; 2.34 A; A=2-165
PDB1AWV; X-ray; 2.34 A; A/B/C/D/E/F=2-165
PDB1BCK; X-ray; 1.80 A; A=1-165
PDB1CWA; X-ray; 2.10 A; A=1-165
PDB1CWB; X-ray; 2.20 A; A=1-165
PDB1CWC; X-ray; 1.86 A; A=1-165
PDB1CWF; X-ray; 1.86 A; A=1-165
PDB1CWH; X-ray; 1.86 A; A=1-165
PDB1CWI; X-ray; 1.90 A; A=1-165
PDB1CWJ; X-ray; 1.80 A; A=1-165
PDB1CWK; X-ray; 1.80 A; A=1-165
PDB1CWL; X-ray; 1.80 A; A=1-165
PDB1CWM; X-ray; 2.00 A; A=1-165
PDB1CWO; X-ray; 1.86 A; A=2-164
PDB1FGL; X-ray; 1.80 A; A=2-164
PDB1M63; X-ray; 2.80 A; C/G=1-165
PDB1M9C; X-ray; 2.00 A; A/B=1-164
PDB1M9D; X-ray; 1.90 A; A/B=1-164
PDB1M9E; X-ray; 1.72 A; A/B=1-163
PDB1M9F; X-ray; 1.73 A; A/B=1-164
PDB1M9X; X-ray; 1.70 A; A/B/E/F=1-164
PDB1M9Y; X-ray; 1.90 A; A/B/E/F=1-164
PDB1MF8; X-ray; 3.10 A; C=1-165
PDB1MIK; X-ray; 1.76 A; A=1-165
PDB1NMK; X-ray; 2.10 A; A/B=1-165
PDB1OCA; NMR; -; A=1-165
PDB1RMH; X-ray; 2.40 A; A/B=2-165
PDB1VBS; X-ray; 2.00 A; A=1-165
PDB1VBT; X-ray; 2.30 A; A/B=1-165
PDB1W8L; X-ray; 1.80 A; A=2-164
PDB1W8M; X-ray; 1.65 A; A=2-164
PDB1W8V; X-ray; 1.70 A; A=2-164
PDB1YND; X-ray; 1.60 A; A/B=1-165
PDB1ZKF; X-ray; 2.55 A; A/B=1-165
PDB2ALF; X-ray; 1.90 A; A=2-164
PDB2CPL; X-ray; 1.63 A; A=2-164
PDB2CYH; X-ray; 1.64 A; A=2-165
PDB2RMA; X-ray; 2.10 A; A/C/E/G/I/K/M/O/Q/S=2-164
PDB2RMB; X-ray; 2.10 A; A/C/E/G/I/K/M/O/Q/S=2-164
PDB2X25; X-ray; 1.20 A; B=2-165
PDB2X2A; X-ray; 1.40 A; A/B=1-165
PDB2X2C; X-ray; 2.41 A; K/M/O/Q/S=1-165
PDB2X2D; X-ray; 1.95 A; B/C=1-165
PDB2XGY; X-ray; 1.80 A; B=1-165
PDB3CYH; X-ray; 1.90 A; A=2-165
PDB3CYS; NMR; -; A=1-165
PDB3K0M; X-ray; 1.25 A; A=1-165
PDB3K0N; X-ray; 1.39 A; A=1-165
PDB3K0O; X-ray; 1.55 A; A=1-165
PDB3K0P; X-ray; 1.65 A; A=1-165
PDB3K0Q; X-ray; 2.32 A; A=1-165
PDB3K0R; X-ray; 2.42 A; A=1-165
PDB4CYH; X-ray; 2.10 A; A=2-165
PDB5CYH; X-ray; 2.10 A; A=2-165
PDBsum1AK4; -; .
PDBsum1AWQ; -; .
PDBsum1AWR; -; .
PDBsum1AWS; -; .
PDBsum1AWT; -; .
PDBsum1AWU; -; .
PDBsum1AWV; -; .
PDBsum1BCK; -; .
PDBsum1CWA; -; .
PDBsum1CWB; -; .
PDBsum1CWC; -; .
PDBsum1CWF; -; .
PDBsum1CWH; -; .
PDBsum1CWI; -; .
PDBsum1CWJ; -; .
PDBsum1CWK; -; .
PDBsum1CWL; -; .
PDBsum1CWM; -; .
PDBsum1CWO; -; .
PDBsum1FGL; -; .
PDBsum1M63; -; .
PDBsum1M9C; -; .
PDBsum1M9D; -; .
PDBsum1M9E; -; .
PDBsum1M9F; -; .
PDBsum1M9X; -; .
PDBsum1M9Y; -; .
PDBsum1MF8; -; .
PDBsum1MIK; -; .
PDBsum1NMK; -; .
PDBsum1OCA; -; .
PDBsum1RMH; -; .
PDBsum1VBS; -; .
PDBsum1VBT; -; .
PDBsum1W8L; -; .
PDBsum1W8M; -; .
PDBsum1W8V; -; .
PDBsum1YND; -; .
PDBsum1ZKF; -; .
PDBsum2ALF; -; .
PDBsum2CPL; -; .
PDBsum2CYH; -; .
PDBsum2RMA; -; .
PDBsum2RMB; -; .
PDBsum2X25; -; .
PDBsum2X2A; -; .
PDBsum2X2C; -; .
PDBsum2X2D; -; .
PDBsum2XGY; -; .
PDBsum3CYH; -; .
PDBsum3CYS; -; .
PDBsum3K0M; -; .
PDBsum3K0N; -; .
PDBsum3K0O; -; .
PDBsum3K0P; -; .
PDBsum3K0Q; -; .
PDBsum3K0R; -; .
PDBsum4CYH; -; .
PDBsum5CYH; -; .
ProteinModelPortalP62937; -; .
SMRP62937; 2-165; .
DIPDIP-6080N; -; .
IntActP62937; 25; .
MINTMINT-4999116; -; .
STRINGP62937; -; .
PhosphoSiteP62937; -; .
SWISS-2DPAGEP62937; -; .
Aarhus/Ghent-2DPAGE2003; NEPHGE; .
DOSAC-COBS-2DPAGEP62937; -; .
OGPP62937; -; .
PMMA-2DPAGEP62937; -; .
REPRODUCTION-2DPAGEIPI00419585; -; .
REPRODUCTION-2DPAGEP62937; -; .
Siena-2DPAGEP62937; -; .
UCD-2DPAGEP62937; -; .
PeptideAtlasP62937; -; .
PRIDEP62937; -; .
EnsemblENST00000468812; ENSP00000419425; ENSG00000196262; .
GeneID5478; -; .
KEGGhsa:5478; -; .
UCSCuc003tlw.1; human; .
CTD5478; -; .
GeneCardsGC07P044803; -; .
H-InvDBHIX0006653; -; .
HGNCHGNC:9253; PPIA; .
HPACAB004655; -; .
MIM123840; gene; .
neXtProtNX_P62937; -; .
eggNOGprNOG18820; -; .
HOVERGENHBG001065; -; .
InParanoidP62937; -; .
OMAGRVCFEL; -; .
OrthoDBEOG4DJJXN; -; .
PhylomeDBP62937; -; .
ReactomeREACT_604; Hemostasis; .
ReactomeREACT_6185; HIV Infection; .
DrugBankDB00091; Cyclosporine; .
DrugBankDB00172; L-Proline; .
NextBio21206; -; .
ArrayExpressP62937; -; .
BgeeP62937; -; .
CleanExHS_PPIA; -; .
GenevestigatorP62937; -; .
GermOnlineENSG00000196262; Homo sapiens; .
GermOnlineENSG00000198618; Homo sapiens; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0005576; C:extracellular region; TAS:Reactome; .
GOGO:0005634; C:nucleus; IDA:UniProtKB; .
GOGO:0042277; F:peptide binding; IEA:UniProtKB-KW; .
GOGO:0003755; F:peptidyl-prolyl cis-trans isomerase activity; IEA:UniProtKB-KW; .
GOGO:0051082; F:unfolded protein binding; TAS:UniProtKB; .
GOGO:0046790; F:virion binding; NAS:UniProtKB; .
GOGO:0030260; P:entry into host cell; TAS:Reactome; .
GOGO:0050900; P:leukocyte migration; TAS:Reactome; .
GOGO:0030168; P:platelet activation; TAS:Reactome; .
GOGO:0002576; P:platelet degranulation; TAS:Reactome; .
GOGO:0006457; P:protein folding; TAS:UniProtKB; .
GOGO:0019047; P:provirus integration; TAS:Reactome; .
GOGO:0045069; P:regulation of viral genome replication; IMP:UniProtKB; .
GOGO:0006278; P:RNA-dependent DNA replication; TAS:Reactome; .
GOGO:0019061; P:uncoating of virus; TAS:Reactome; .
InterProIPR015891; Cyclophilin-like; .
InterProIPR020892; Pep-Pro_Isoase_cyclophilin_CS; .
InterProIPR002130; PPIase_cyclophilin; .
Gene3DG3DSA:2.40.100.10; PPIase_cyclophilin; 1; .
PfamPF00160; Pro_isomerase; 1; .
PRINTSPR00153; CSAPPISMRASE; .
SUPFAMSSF50891; CSA_PPIase; 1; .
PROSITEPS00170; CSA_PPIASE_1; 1; .
PROSITEPS50072; CSA_PPIASE_2; 1; .



SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server