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Protein list for World-2DPAGE Repository [0027] GIPRDN_4-7 
glomerular protein lysates of a GIPRdn-tg animal  {Mus musculus (Mouse)}


(map ID: submission_0027:GIPRDN_4-7)
Release: 3 -- 23 November 2011

Gel Preparation (local documents: informatics )


Protein list for giprdn_4-7   26 identified spots / 34 protein entries

Gene Name Description Accession n° Spot ID Exp pI Exp Mw Mapping Methods Comment Topics References
- RecName: Full=Ig kappa chain C region; P01837
(IGKC_MOUSE)
28
6.37
23'000
{MS/MS, PMF}
identification,expression
1
Acadsb RecName: Full=Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial; Short=SBCAD; EC=1.3.99.-; AltName: Full=2-methyl branched chain acyl-CoA dehydrogenase; Short=2-MEBCAD; AltName: Full=2-methylbutyryl-coenzyme A dehydrogenase; Short=2-methylbutyryl-CoA dehydrogenase; Flags: Precursor; Q9DBL1
(ACDSB_MOUSE)
25
5.45
35'000
{MS/MS, PMF}
Identification
1
Actg1 RecName: Full=Actin, cytoplasmic 2; AltName: Full=Gamma-actin; Contains: RecName: Full=Actin, cytoplasmic 2, N-terminally processed; P63260
(ACTG_MOUSE)
16
20
17
18
40
5.17
5.52
5.17
5.03
5.27
42'000
38'000
41'000
41'000
16'000
{MS/MS, PMF}
{MS/MS, PMF}
{MS/MS, PMF}
{MS/MS, PMF}
{MS/MS, PMF}
identification,expression
Identification
identification,expression
identification,expression
identification,expression
1
1
1
1
1
Anxa3 RecName: Full=Annexin A3; AltName: Full=35-alpha calcimedin; AltName: Full=Annexin III; AltName: Full=Annexin-3; AltName: Full=Lipocortin III; AltName: Full=Placental anticoagulant protein III; Short=PAP-III; O35639
(ANXA3_MOUSE)
25
5.45
35'000
{MS/MS, PMF}
Identification
1
Anxa4 RecName: Full=Annexin A4; AltName: Full=Annexin IV; AltName: Full=Annexin-4; P97429
(ANXA4_MOUSE)
27
5.36
31'000
{MS/MS, PMF}
identification,expression
1
Atp5h RecName: Full=ATP synthase subunit d, mitochondrial; Short=ATPase subunit d; Q9DCX2
(ATP5H_MOUSE)
36
5.27
21'000
{MS/MS, PMF}
identification,expression
1
Bckdha RecName: Full=2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial; EC=1.2.4.4; AltName: Full=Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; Short=BCKDE1A; Short=BCKDH E1-alpha; Flags: Precursor; P50136
(ODBA_MOUSE)
15
6.35
45'000
{MS/MS, PMF}
identification,expression
1
Blmh RecName: Full=Bleomycin hydrolase; Short=BH; Short=BLM hydrolase; Short=BMH; EC=3.4.22.40; Q8R016
(BLMH_MOUSE)
13
6.32
51'000
{PMF}
identification,expression
1
C4b RecName: Full=Complement C4-B; Contains: RecName: Full=Complement C4 beta chain; Contains: RecName: Full=Complement C4 alpha chain; Contains: RecName: Full=C4a anaphylatoxin; Contains: RecName: Full=Complement C4 gamma chain; Flags: Precursor; P01029
(CO4B_MOUSE)
35
6.44
22'000
{PMF}
identification,expression
1
Commd8 RecName: Full=COMM domain-containing protein 8; Q9CZG3
(COMD8_MOUSE)
36
5.27
21'000
{MS/MS, PMF}
Identification
1
Des RecName: Full=Desmin; P31001
(DESM_MOUSE)
14
4.71
45'000
{MS/MS, PMF}
Identification
1
Dpysl2 RecName: Full=Dihydropyrimidinase-related protein 2; Short=DRP-2; AltName: Full=Unc-33-like phosphoprotein 2; Short=ULIP-2; O08553
(DPYL2_MOUSE)
5
6.23
63'000
{MS/MS, PMF}
identification,expression
1
Eif3j RecName: Full=Eukaryotic translation initiation factor 3 subunit J; Short=eIF3j; AltName: Full=Eukaryotic translation initiation factor 3 subunit 1; AltName: Full=eIF-3-alpha; AltName: Full=eIF3 p35; Q66JS6
(EIF3J_MOUSE)
22
4.61
35'000
{MS/MS, PMF}
identification,expression
1
Fgg RecName: Full=Fibrinogen gamma chain; Flags: Precursor; Q8VCM7
(FIBG_MOUSE)
10
5.43
50'000
{MS/MS, PMF}
identification,expression
1
Gpx1 RecName: Full=Glutathione peroxidase 1; Short=GPx-1; Short=GSHPx-1; EC=1.11.1.9; AltName: Full=Cellular glutathione peroxidase; P11352
(GPX1_MOUSE)
34
5.66
22'000
{MS/MS, PMF}
Identification
1
Gpx3 RecName: Full=Glutathione peroxidase 3; Short=GPx-3; Short=GSHPx-3; EC=1.11.1.9; AltName: Full=Plasma glutathione peroxidase; Short=GPx-P; Short=GSHPx-P; Flags: Precursor; P46412
(GPX3_MOUSE)
34
5.66
22'000
{MS/MS, PMF}
identification,expression
1
Hnrnpd RecName: Full=Heterogeneous nuclear ribonucleoprotein D0; Short=hnRNP D0; AltName: Full=AU-rich element RNA-binding protein 1; Q60668
(HNRPD_MOUSE)
15
6.35
45'000
{MS/MS, PMF}
Identification
1
Hnrnph1 RecName: Full=Heterogeneous nuclear ribonucleoprotein H; Short=hnRNP H; Contains: RecName: Full=Heterogeneous nuclear ribonucleoprotein H, N-terminally processed; O35737
(HNRH1_MOUSE)
29
11
6.51
5.92
23'000
51'000
{MS/MS, PMF}
{MS/MS, PMF}
identification,expression
identification,expression
1
1
Hnrnph2 RecName: Full=Heterogeneous nuclear ribonucleoprotein H2; Short=hnRNP H2; AltName: Full=Heterogeneous nuclear ribonucleoprotein H'; Short=hnRNP H'; P70333
(HNRH2_MOUSE)
11
29
5.92
6.51
51'000
23'000
{MS/MS, PMF}
{MS/MS, PMF}
Identification
Identification
1
1
Hspd1 RecName: Full=60 kDa heat shock protein, mitochondrial; AltName: Full=60 kDa chaperonin; AltName: Full=Chaperonin 60; Short=CPN60; AltName: Full=HSP-65; AltName: Full=Heat shock protein 60; Short=HSP-60; Short=Hsp60; AltName: Full=Mitochondrial matrix protein P1; Flags: Precursor; P63038
(CH60_MOUSE)
7
5.33
58'000
{MS/MS, PMF}
identification,expression
1
Idh3a RecName: Full=Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; EC=1.1.1.41; AltName: Full=Isocitric dehydrogenase subunit alpha; AltName: Full=NAD(+)-specific ICDH subunit alpha; Flags: Precursor; Q9D6R2
(IDH3A_MOUSE)
20
5.52
38'000
{MS/MS, PMF}
identification,expression
1
Mfge8 RecName: Full=Lactadherin; AltName: Full=MFGM; AltName: Full=Milk fat globule-EGF factor 8; Short=MFG-E8; AltName: Full=SED1; AltName: Full=Sperm surface protein SP47; Short=MP47; Flags: Precursor; P21956
(MFGM_MOUSE)
9
5.92
56'000
{PMF}
identification,expression
1
Myl9 RecName: Full=Myosin regulatory light polypeptide 9; AltName: Full=Myosin regulatory light chain 2, smooth muscle isoform; AltName: Full=Myosin regulatory light chain 9; Q9CQ19
(MYL9_MOUSE)
42
4.51
19'000
{MS/MS, PMF}
identification,expression
1
Pdhb RecName: Full=Pyruvate dehydrogenase E1 component subunit beta, mitochondrial; Short=PDHE1-B; EC=1.2.4.1; Flags: Precursor; Q9D051
(ODPB_MOUSE)
25
5.45
35'000
{MS/MS, PMF}
identification,expression
1
Prph RecName: Full=Peripherin; P15331
(PERI_MOUSE)
14
4.71
45'000
{MS/MS, PMF}
Identification
1
Pter RecName: Full=Phosphotriesterase-related protein; EC=3.1.-.-; AltName: Full=Parathion hydrolase-related protein; Q60866
(PTER_MOUSE)
21
6.35
39'000
{MS/MS, PMF}
identification,expression
1
Rad17 RecName: Full=Cell cycle checkpoint protein RAD17; Q6NXW6
(RAD17_MOUSE)
11
5.92
51'000
{PMF}
Identification
1
Sag RecName: Full=S-arrestin; AltName: Full=48 kDa protein; AltName: Full=Retinal S-antigen; Short=S-AG; AltName: Full=Rod photoreceptor arrestin; P20443
(ARRS_MOUSE)
21
6.35
39'000
{MS/MS, PMF}
Identification
1
Sardh RecName: Full=Sarcosine dehydrogenase, mitochondrial; Short=SarDH; EC=1.5.99.1; Flags: Precursor; Q99LB7
(SARDH_MOUSE)
1
6.23
97'000
{PMF}
identification,expression
1
Sdha RecName: Full=Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; EC=1.3.5.1; AltName: Full=Flavoprotein subunit of complex II; Short=Fp; Flags: Precursor; Q8K2B3
(DHSA_MOUSE)
3
6.32
78'000
{MS/MS, PMF}
identification,expression
1
Sptb RecName: Full=Spectrin beta chain, erythrocyte; AltName: Full=Beta-I spectrin; P15508
(SPTB1_MOUSE)
9
5.92
56'000
{PMF}
Identification
1
Tpm1 RecName: Full=Tropomyosin alpha-1 chain; AltName: Full=Alpha-tropomyosin; AltName: Full=Tropomyosin-1; P58771
(TPM1_MOUSE)
24
4.61
34'000
{MS/MS, PMF}
identification,expression
1
Tufm RecName: Full=Elongation factor Tu, mitochondrial; Flags: Precursor; Q8BFR5
(EFTU_MOUSE)
15
6.35
45'000
{MS/MS, PMF}
Identification
1
Vim RecName: Full=Vimentin; P20152
(VIME_MOUSE)
14
4.71
45'000
{MS/MS, PMF}
identification,expression
1
Mapping legends: { MS/MS } => 'Tandem mass spectrometry', { PMF } => 'Peptide mass fingerprinting'



Cited references

# PubMed/Medline Authors Location
[1] doi:10.1002/prca.201000103 Blutke A., Block C., Berendt F., Herbach N., Kemter E., Amann K., Frolich T., Arnold G.J., Wanke R.; Proteomics - Clinical Applications 5(6):375-381 (2011).
''Differential glomerular proteome analysis of two murine nephropathy models at onset of albuminuria'';


Gel experimental information

Information Type Data
Map name GIPRDN_4-7 {glomerular protein lysates of a GIPRdn-tg animal}
Dimension 2-D
Related documents Gel Preparation (local documents: informatics )
species Mus musculus (Mouse) / TaxID:10090
pI start: 4.00 - end: 7.00
Mw start: 10000 - end: 200000
Informatics Software DeCyder Differential Analysis Software v6.5
Number of identified spots 26
Number of identified proteins 34
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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server