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Protein list for World-2DPAGE Repository [0003] PHENOL_2 
Phenol 2  {Arabidopsis thaliana (Mouse-ear cress)}
tissue: Leaf


(map ID: submission_0003:PHENOL_2)
Release: 3 -- 15 November 2011

Gel Preparation


Protein list for phenol_2   29 identified spots / 24 protein entries

Gene Name Description Accession n° Spot ID Exp pI Exp Mw Mapping Methods Comment Topics References
- Q3E9N6 Q3E9N6
(Q3E9N6)
35
5.94
17'970
{MS/MS, PMF}
Identification
1
At5g29771 SubName: Full=Putative ribosomal protein S1; SubName: Full=Ribosomal protein S1; SubName: Full=Small subunit ribosomal protein S1; Q93VC7
(Q93VC7_ARATH)
15
5.40
47'360
{MS/MS, PMF}
Identification
1
CTIMC RecName: Full=Triosephosphate isomerase, cytosolic; Short=TIM; Short=Triose-phosphate isomerase; EC=5.3.1.1; P48491
(TPIS_ARATH)
63
52
6.60
6.17
18'210
30'130
{MS/MS, PMF}
{MS/MS, PMF}
Identification
Identification
1
1
FBA1 RecName: Full=Probable fructose-bisphosphate aldolase 1, chloroplastic; EC=4.1.2.13; Flags: Precursor; Q9SJU4
(ALFC1_ARATH)
41
5.99
39'530
{MS/MS, PMF}
Identification
1
FTSH2 RecName: Full=ATP-dependent zinc metalloprotease FTSH 2, chloroplastic; Short=AtFTSH2; EC=3.4.24.-; AltName: Full=Protein VARIEGATED 2; Flags: Precursor; O80860
(FTSH2_ARATH)
16
31
5.67
5.73
72'230
72'170
{MS/MS, PMF}
{MS/MS, PMF}
Identification
Identification
1
1
GAPA RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic; EC=1.2.1.13; AltName: Full=NADP-dependent glyceraldehydephosphate dehydrogenase subunit A; Flags: Precursor; P25856
(G3PA_ARATH)
91
7.34
42'210
{MS/MS, PMF}
Identification
1
HCF136 RecName: Full=Photosystem II stability/assembly factor HCF136, chloroplastic; Flags: Precursor; O82660
(P2SAF_ARATH)
28
5.82
41'670
{MS/MS, PMF}
Identification
1
HSC70-1 RecName: Full=Heat shock cognate 70 kDa protein 1; Short=Hsc70.1; P22953
(HSP71_ARATH)
19
5.58
78'660
{MS/MS, PMF}
Identification
1
HSC70-3 RecName: Full=Heat shock cognate 70 kDa protein 3; Short=Hsc70.3; O65719
(HSP73_ARATH)
21
20
5.49
5.53
78'600
78'300
{MS/MS, PMF}
{MS/MS, PMF}
Identification
Identification
1
1
LFNR2 RecName: Full=Ferredoxin--NADP reductase, leaf isozyme 2, chloroplastic; EC=1.18.1.2; AltName: Full=Leaf FNR 2; Short=AtLFNR2; Short=FNR-2; Flags: Precursor; Q8W493
(FNRL2_ARATH)
81
6.93
37'730
{MS/MS, PMF}
Identification
1
PGK1 RecName: Full=Phosphoglycerate kinase 1, chloroplastic; EC=2.7.2.3; Flags: Precursor; Q9LD57
(PGKH1_ARATH)
13
5.64
45'000
{MS/MS, PMF}
Identification
1
RBCS-1A RecName: Full=Ribulose bisphosphate carboxylase small chain 1A, chloroplastic; Short=RuBisCO small subunit 1A; EC=4.1.1.39; Flags: Precursor; P10795
(RBS1A_ARATH)
70
6.76
14'340
{MS/MS, PMF}
Identification
1
RBCS-1B RecName: Full=Ribulose bisphosphate carboxylase small chain 1B, chloroplastic; Short=RuBisCO small subunit 1B; EC=4.1.1.39; Flags: Precursor; P10796
(RBS1B_ARATH)
69
6.72
15'130
{MS/MS, PMF}
Identification
1
RCA RecName: Full=Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic; Short=RA; Short=RuBisCO activase; Flags: Precursor; P10896
(RCA_ARATH)
54
43
6.08
5.98
44'460
44'770
{MS/MS, PMF}
{MS/MS, PMF}
Identification
Identification
1
1
rps5 RecName: Full=30S ribosomal protein S5, chloroplastic; Flags: Precursor; P93014
(RR5_ARATH)
87
7.17
35'180
{MS/MS, PMF}
Identification
1
T6J4.17 SubName: Full=At1g13440/F13B4_8; SubName: Full=Glyceraldehyde 3-phosphate dehydrogenase; SubName: Full=Putative glyceraldehyde-3-phosphate dehydrogenase; Q9FX54
(Q9FX54_ARATH)
90
89
7.26
7.20
42'610
42'610
{MS/MS, PMF}
{MS/MS, PMF}
Identification
Identification
1
1
THFS RecName: Full=Formate--tetrahydrofolate ligase; EC=6.3.4.3; AltName: Full=10-formyletrahydrofolate synthetase; Short=FHS; Short=FTHFS; AltName: Full=Formyltetrahydrofolate synthetase; Q9SPK5
(FTHS_ARATH)
82
6.92
78'090
{MS/MS, PMF}
Identification
1
TIM RecName: Full=Triosephosphate isomerase, chloroplastic; Short=TIM; Short=Triose-phosphate isomerase; EC=5.3.1.1; Flags: Precursor; Q9SKP6
(TPIC_ARATH)
51
6.13
30'750
{MS/MS, PMF}
Identification
1
VHA-E1 RecName: Full=V-type proton ATPase subunit E1; Short=V-ATPase subunit E1; AltName: Full=Protein EMBRYO DEFECTIVE 2448; AltName: Full=Vacuolar H(+)-ATPase subunit E isoform 1; AltName: Full=Vacuolar proton pump subunit E1; Q39258
(VATE1_ARATH)
66
6.67
33'110
{MS/MS, PMF}
Identification
1
OrderedLocusNames=At1g32060 RecName: Full=Phosphoribulokinase, chloroplastic; Short=PRK; Short=PRKase; EC=2.7.1.19; AltName: Full=Phosphopentokinase; Flags: Precursor; P25697
(KPPR_ARATH)
30
5.75
43'750
{MS/MS, PMF}
Identification
1
OrderedLocusNames=At2g24060 RecName: Full=Translation initiation factor IF-3; O82234
(O82234_ARATH)
29
5.85
39'600
{MS/MS, PMF}
Identification
1
OrderedLocusNames=At2g37660 RecName: Full=Uncharacterized protein At2g37660, chloroplastic; Flags: Precursor; O80934
(Y2766_ARATH)
40
6.06
32'450
{MS/MS, PMF}
Identification
1
OrderedLocusNames=At3g55800 RecName: Full=Sedoheptulose-1,7-bisphosphatase, chloroplastic; EC=3.1.3.37; AltName: Full=SED(1,7)P2ase; AltName: Full=Sedoheptulose bisphosphatase; Short=SBPase; Flags: Precursor; P46283
(S17P_ARATH)
12
5.55
41'420
{MS/MS, PMF}
Identification
1
OrderedLocusNames=At5g49910 SubName: Full=Heat shock protein 70; SubName: Full=Heat shock protein 70-2; SubName: Full=Putative heat shock protein 70; Q9LTX9
(Q9LTX9_ARATH)
2
5.34
78'910
{MS/MS, PMF}
Identification
1
Mapping legends: { MS/MS } => 'Tandem mass spectrometry', { PMF } => 'Peptide mass fingerprinting'



Cited references

# PubMed/Medline Authors Location
[1] 18656559 [doi:10.1016/j.jprot.2008.06.012] Maldonado A.M., Echevarria-Zomeno S., Jean-Baptiste S., Hernandez M., Jorrin-Novo J.V.; J. Proteomics 71(4):461-472 (2008).
''Evaluation of three different protocols of protein extraction for Arabidopsis thaliana leaf proteome analysis by two-dimensional electrophoresis'';


Gel experimental information

Information Type Data
Map name PHENOL_2 {Phenol 2}
Dimension 2-D
Related documents Gel Preparation
Strain Comment [species] Columbia ecotype, Col-0 [Arabidopsis thaliana (Mouse-ear cress) / TaxID:3702]
Tissue (Swiss-Prot definition) Leaf
pI start: 5.00 - end: 8.00
Mw start: 12000 - end: 100000
Number of detected spots 677
Number of identified spots 29
Number of identified proteins 24
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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server