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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: ATPD_HUMAN




SWISS-2DPAGE:  ATPD_HUMAN


ATPD_HUMAN


General information about the entry
View entry in simple text format
Entry nameATPD_HUMAN
Primary accession numberP30049
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onMay 15, 2003 (version 4)
General Annotations were last modified on May 19, 2011 (version 13)
Name and origin of the protein
DescriptionRecName: Full=ATP synthase subunit delta, mitochondrial; AltName: Full=F-ATPase delta subunit; Flags: Precursor;.
Gene nameName=ATP5D
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=93162045; PubMed=1286669; [NCBI, ExPASy, EBI, Israel, Japan]
Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F., Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel R.D., Hughes G.J.
''''''Human liver protein map: a reference database established by microsequencing and gel comparison'';'';''
Electrophoresis 13(1):992-1001(1992)
[2]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
[3]   MAPPING ON GEL
MEDLINE=97295305; PubMed=9150947; [NCBI, ExPASy, EBI, Israel, Japan]
Sarto C., Marocchi A., Sanchez J.-C., Giannone D., Frutiger S., Golaz O., Wilkins M.R., Doro G., Cappellano F., Hughes G.J., Hochstrasser D.F., Mocarelli P.
''''''Renal cell carcinoma and normal kidney protein expression'';'';''
Electrophoresis 18(1):599-604(1997)
[4]   MAPPING ON GEL
Tonella L., Hoogland C., Appel R.D., Sanchez J.-C., Hochstrasser D.F.
Submitted (JUL-1997) to SWISS-2DPAGE
[5]   MAPPING ON GEL
MEDLINE=96314065; PubMed=8740185; [NCBI, ExPASy, EBI, Israel, Japan]
Lutomski D., Caron M., Cornillot J.D., Bourin P., Dupuy C., Pontet M., Bladier D., Joubert-Caron R.
''''''Identification of different galectins by immunoblotting after two- dimensional polyacrylamide get electrophoresis with immobilized pH gradients'';'';''
Electrophoresis 17(1):600-606(1996)
[6]   MAPPING ON GEL
MEDLINE=98163346; PubMed=9504818; [NCBI, ExPASy, EBI, Israel, Japan]
Reymond M.A., Sanchez J.-C., Hughes G.J., Riese J., Tortola S., Peinado M.A., Kirchner T., Hohenberger W., Hochstrasser D.F., Kockerling F.
''''''Standardized characterization of gene expression in human colorectal epithelium by two-dimensional electrophoresis'';'';''
Electrophoresis 18(1):2842-2848(1997)
[7]   MAPPING ON GEL
Demalte-Annessi I., Sanchez J.-C., Hoogland C., Rouge V., Binz P.-A., Appel R.D., Hochstrasser D.F.
Submitted (JAN-1999) to SWISS-2DPAGE
Comments
  • SUBUNIT: ONE OF THE 5 CHAINS OF THE ENZYMATIC COMPONENT OF THE MITOCHONDRIAL ATPASE COMPLEX
2D PAGE maps for identified proteins
How to interpret a protein

CEC_HUMAN {Colorectal epithelia cells}
Homo sapiens (Human)
Tissue: Colon epithelium
CEC_HUMAN
  map experimental info
  protein estimated location
 
CEC_HUMAN

MAP LOCATIONS:
pI=4.50; Mw=13360

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [6].



DLD1_HUMAN {Colorectal adenocarcinoma cell line (DL-1)}
Homo sapiens (Human)
Tissue: Colon adenocarcinoma
DLD1_HUMAN
  map experimental info
  protein estimated location
 
DLD1_HUMAN

MAP LOCATIONS:
pI=4.50; Mw=13360

MAPPING (identification):
MATCHING WITH THE HEPG2 MASTER GEL [7].



HEPG2_HUMAN {HepG2}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2_HUMAN
  map experimental info
  protein estimated location
 
HEPG2_HUMAN

MAP LOCATIONS:
pI=4.50; Mw=13360

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



HL60_HUMAN {Promyelocytic leukemia cells}
Homo sapiens (Human)
Tissue: Promyelocytic leukemia
HL60_HUMAN
  map experimental info
  protein estimated location
 
HL60_HUMAN

MAP LOCATIONS:
pI=4.40; Mw=12724

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [4][5].



KIDNEY_HUMAN {Kidney}
Homo sapiens (Human)
Tissue: Kidney
KIDNEY_HUMAN
  map experimental info
  protein estimated location
 
KIDNEY_HUMAN

MAP LOCATIONS:
pI=4.50; Mw=13672

MAPPING (identification):
MICROSEQUENCING [3].



LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=4.50; Mw=13244

MAPPING (identification):
MICROSEQUENCING [1].



LYMPHOMA_HUMAN {Lymphoma}
Homo sapiens (Human)
Tissue: Lymphoma
LYMPHOMA_HUMAN
  map experimental info
  protein estimated location
 
LYMPHOMA_HUMAN

MAP LOCATIONS:
pI=4.53; Mw=13414

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].



U937_HUMAN {Macrophage Like Cell Line}
Homo sapiens (Human)
Tissue: Histiocytic lymphoma
U937_HUMAN
  map experimental info
  protein estimated location
 
U937_HUMAN

MAP LOCATIONS:
pI=4.22; Mw=12868
pI=4.25; Mw=12755

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
OGP-WWWP30049.
Siena-2DPAGEP30049; ATPD_HUMAN.
UCD-2DPAGEP30049; ATPD_HUMAN.
UniProtKB/Swiss-ProtP30049; ATPD_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameATPD_HUMAN
Primary accession numberP30049
Secondary accession number(s) D6W5Y3 Q6FG90
Sequence was last modified on October 1, 1994 (version 2)
Annotations were last modified on October 19, 2011 (version 115)
Name and origin of the protein
DescriptionRecName: Full=ATP synthase subunit delta, mitochondrial; AltName: Full=F-ATPase delta subunit; Flags: Precursor;
Gene nameName=ATP5D
Encoded onName=ATP5D
KeywordsATP synthesis; CF(1); Complete proteome; Direct protein sequencing; Hydrogen ion transport; Ion transport; Isopeptide bond; Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome; Transit peptide; Transport; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLS87916; AAA08055.1; -; mRNA
EMBLS87918; ABB76284.1; -; mRNA
EMBLX63422; CAA45016.1; -; mRNA
EMBLX63423; CAA45017.1; -; mRNA
EMBLCR542218; CAG47014.1; -; mRNA
EMBLAC004221; AAC04304.1; -; Genomic_DNA
EMBLCH471139; EAW69532.1; -; Genomic_DNA
EMBLCH471139; EAW69533.1; -; Genomic_DNA
EMBLCH471139; EAW69534.1; -; Genomic_DNA
EMBLBC002389; AAH02389.1; -; mRNA
EMBLBC004426; AAH04426.1; -; mRNA
EMBLBC018079; AAH18079.1; -; mRNA
IPIIPI00024920; -; .
PIRS22348; S22348; .
RefSeqNP_001001975.1; NM_001001975.1; .
RefSeqNP_001678.1; NM_001687.4; .
UniGeneHs.418668; -; .
ProteinModelPortalP30049; -; .
SMRP30049; 37-167; .
IntActP30049; 1; .
STRINGP30049; -; .
PhosphoSiteP30049; -; .
SWISS-2DPAGEP30049; -; .
OGPP30049; -; .
UCD-2DPAGEP30049; -; .
PeptideAtlasP30049; -; .
PRIDEP30049; -; .
EnsemblENST00000215375; ENSP00000215375; ENSG00000099624; .
EnsemblENST00000395633; ENSP00000378995; ENSG00000099624; .
GeneID513; -; .
KEGGhsa:513; -; .
UCSCuc002lrn.1; human; .
CTD513; -; .
GeneCardsGC19P001016; -; .
H-InvDBHIX0014575; -; .
HGNCHGNC:837; ATP5D; .
HPAHPA002865; -; .
MIM603150; gene; .
neXtProtNX_P30049; -; .
PharmGKBPA25127; -; .
eggNOGprNOG20085; -; .
GeneTreeENSGT00390000017576; -; .
HOGENOMHBG663981; -; .
HOVERGENHBG001856; -; .
InParanoidP30049; -; .
OMAVVQVNIP; -; .
OrthoDBEOG4894NT; -; .
PhylomeDBP30049; -; .
ReactomeREACT_6305; Respiratory electron transport; ATP synthesis by chemiosmotic coupling; and heat production by uncoupling proteins
DrugBankDB01189; Desflurane; .
DrugBankDB00228; Enflurane; .
DrugBankDB01159; Halothane; .
DrugBankDB00753; Isoflurane; .
DrugBankDB01028; Methoxyflurane; .
DrugBankDB01236; Sevoflurane; .
NextBio2123; -; .
ArrayExpressP30049; -; .
BgeeP30049; -; .
CleanExHS_ATP5D; -; .
GenevestigatorP30049; -; .
GermOnlineENSG00000099624; Homo sapiens; .
GOGO:0005759; C:mitochondrial matrix; TAS:Reactome; .
GOGO:0000275; C:mitochondrial proton-transporting ATP synthase complex; catalytic core F(1); NAS:UniProtKB
GOGO:0046933; F:hydrogen ion transporting ATP synthase activity; rotational mechanism; IEA:InterPro
GOGO:0046961; F:proton-transporting ATPase activity; rotational mechanism; IEA:InterPro
GOGO:0042776; P:mitochondrial ATP synthesis coupled proton transport; NAS:UniProtKB; .
GOGO:0006119; P:oxidative phosphorylation; NAS:UniProtKB; .
GOGO:0022904; P:respiratory electron transport chain; TAS:Reactome; .
GOGO:0046688; P:response to copper ion; NAS:UniProtKB; .
InterProIPR001469; ATPase_F1-cplx_dsu/esu; .
InterProIPR020547; ATPase_F1-cplx_dsu/esu_C; .
InterProIPR020546; ATPase_F1-cplx_dsu/esu_N; .
Gene3DG3DSA:2.60.15.10; ATPase_F1_d/e; 1; .
PANTHERPTHR13822; ATPase_F1_d/e; 1; .
PfamPF02823; ATP-synt_DE_N; 1; .
ProDomPD000944; ATPase_F1-cplx_dsu/esu; 1; .
SUPFAMSSF46604; ATPsynt_DE; 1; .
SUPFAMSSF51344; ATPsynt_DE; 1; .
TIGRFAMsTIGR01216; ATP_synt_epsi; 1; .



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