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SWISS-2DPAGE

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SWISS-2DPAGE 
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Protein list for SWISS-2DPAGE KIDNEY_HUMAN 
Kidney  {Homo sapiens (Human)}
tissue: Kidney


(map ID: c74e4f03-6c7e-4d7e-a88e-05bf097aca67)
Release: 19.2 -- 09 November 2011

Sample Preparation / Gel Related Documents


Protein list for kidney_human   44 identified spots / 28 protein entries

Gene Name Description Accession n° Spot ID Exp pI Exp Mw Mapping Methods Comment Topics References
ACADS RecName: Full=Short-chain specific acyl-CoA dehydrogenase, mitochondrial; Short=SCAD; EC=1.3.8.1; AltName: Full=Butyryl-CoA dehydrogenase; Flags: Precursor; P16219
(ACADS_HUMAN)
2D-000NB3
6.41
37'376
{Gm}
-
1
ACTB RecName: Full=Actin, cytoplasmic 1; AltName: Full=Beta-actin; Contains: RecName: Full=Actin, cytoplasmic 1, N-terminally processed; P60709
(ACTB_HUMAN)
2D-000N5T
2D-000N67
2D-000N6J
2D-000N5U
5.19
5.14
5.12
5.26
41'311
41'503
41'407
41'600
{Im,Gm}
{Im,Gm}
{Im,Gm}
{Im,Gm}
-
-
-
-
1
1
1
1
ALB RecName: Full=Serum albumin; Flags: Precursor; P02768
(ALBU_HUMAN)
2D-000MQ3
2D-000MPU
2D-000MQ5
2D-000MPV
5.58
5.37
5.80
5.38
65'700
65'917
65'700
65'917
{Gm}
{Gm}
{Gm}
{Gm}
-
-
-
-
1
1
1
1
ATP5B RecName: Full=ATP synthase subunit beta, mitochondrial; EC=3.6.3.14; Flags: Precursor; P06576
(ATPB_HUMAN)
2D-000N0D
2D-000N0X
5.03
4.99
47'989
48'244
{Aa}
{Aa}
-
-
1
1
ATP5D RecName: Full=ATP synthase subunit delta, mitochondrial; AltName: Full=F-ATPase delta subunit; Flags: Precursor; P30049
(ATPD_HUMAN)
2D-000NYM
4.50
13'672
{Mi}
-
1
CALR RecName: Full=Calreticulin; AltName: Full=CRP55; AltName: Full=Calregulin; AltName: Full=Endoplasmic reticulum resident protein 60; Short=ERp60; AltName: Full=HACBP; AltName: Full=grp60; Flags: Precursor; P27797
(CALR_HUMAN)
2D-000MS2
4.40
58'560
{Gm,Aa}
-
1
CAT RecName: Full=Catalase; EC=1.11.1.6; P04040
(CATA_HUMAN)
2D-000MU6
2D-000MU8
6.76
6.84
55'800
55'544
{Gm}
{Gm}
-
-
1
1
COX5A RecName: Full=Cytochrome c oxidase subunit 5A, mitochondrial; AltName: Full=Cytochrome c oxidase polypeptide Va; Flags: Precursor; P20674
(COX5A_HUMAN)
2D-000O0T
4.90
12'400
{Gm,Aa}
-
1
CRYAA RecName: Full=Alpha-crystallin A chain; AltName: Full=Heat shock protein beta-4; Short=HspB4; Contains: RecName: Full=Alpha-crystallin A chain, short form; P02489
(CRYAA_HUMAN)
2D-000NVY
6.85
17'247
{Aa}
-
1
CRYAB RecName: Full=Alpha-crystallin B chain; AltName: Full=Alpha(B)-crystallin; AltName: Full=Heat shock protein beta-5; Short=HspB5; AltName: Full=Renal carcinoma antigen NY-REN-27; AltName: Full=Rosenthal fiber component; P02511
(CRYAB_HUMAN)
2D-000NWM
7.23
15'636
{Mi}
-
1
EEF1B2 RecName: Full=Elongation factor 1-beta; Short=EF-1-beta; P24534
(EF1B_HUMAN)
2D-000NK7
4.57
28'635
{Gm}
-
1
GLUD1 RecName: Full=Glutamate dehydrogenase 1, mitochondrial; Short=GDH 1; EC=1.4.1.3; Flags: Precursor; P00367
(DHE3_HUMAN)
2D-000N0L
2D-000N0H
6.72
6.81
48'612
48'500
{Gm}
{Gm}
-
-
1
1
GPX3 RecName: Full=Glutathione peroxidase 3; Short=GPx-3; Short=GSHPx-3; EC=1.11.1.9; AltName: Full=Extracellular glutathione peroxidase; AltName: Full=Plasma glutathione peroxidase; Short=GPx-P; Short=GSHPx-P; Flags: Precursor; P22352
(GPX3_HUMAN)
2D-000NT4
2D-000NT7
5.38
5.36
22'786
22'786
{Mi,PMF}
{Mi,PMF}
Expression
Expression
2
2
GSTP1 RecName: Full=Glutathione S-transferase P; EC=2.5.1.18; AltName: Full=GST class-pi; AltName: Full=GSTP1-1; P09211
(GSTP1_HUMAN)
2D-000NSN
5.38
23'171
{Gm}
-
1
HSPA9 RecName: Full=Stress-70 protein, mitochondrial; AltName: Full=75 kDa glucose-regulated protein; Short=GRP-75; AltName: Full=Heat shock 70 kDa protein 9; AltName: Full=Mortalin; Short=MOT; AltName: Full=Peptide-binding protein 74; Short=PBP74; Flags: Precursor; P38646
(GRP75_HUMAN)
2D-000MPF
2D-000MPG
5.27
5.30
66'791
66'571
{Mi,Aa}
{Mi,Aa}
-
-
1
1
HSPD1 RecName: Full=60 kDa heat shock protein, mitochondrial; AltName: Full=60 kDa chaperonin; AltName: Full=Chaperonin 60; Short=CPN60; AltName: Full=Heat shock protein 60; Short=HSP-60; Short=Hsp60; AltName: Full=HuCHA60; AltName: Full=Mitochondrial matrix protein P1; AltName: Full=P60 lymphocyte protein; Flags: Precursor; P10809
(CH60_HUMAN)
2D-000MTQ
2D-000MTT
2D-000MTP
5.23
5.14
5.11
55'929
56'057
56'186
{Im,Gm,Aa}
{Im,Gm,Aa}
{Im,Gm,Aa}
-
-
-
1
1
1
LGALS1 RecName: Full=Galectin-1; Short=Gal-1; AltName: Full=14 kDa laminin-binding protein; Short=HLBP14; AltName: Full=14 kDa lectin; AltName: Full=Beta-galactoside-binding lectin L-14-I; AltName: Full=Galaptin; AltName: Full=HBL; AltName: Full=HPL; AltName: Full=Lactose-binding lectin 1; AltName: Full=Lectin galactoside-binding soluble 1; AltName: Full=Putative MAPK-activating protein PM12; AltName: Full=S-Lac lectin 1; P09382
(LEG1_HUMAN)
2D-000O18
5.04
11'822
{Gm}
-
1
MYL1 RecName: Full=Myosin light chain 1/3, skeletal muscle isoform; Short=MLC1/MLC3; Short=MLC1F/MLC3F; AltName: Full=Myosin light chain alkali 1/2; Short=Myosin light chain A1/A2; P05976
(MYL1_HUMAN)
2D-000NUH
5.12
20'319
{Aa}
-
1
NDUFA8 RecName: Full=NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8; AltName: Full=Complex I-19kD; Short=CI-19kD; AltName: Full=Complex I-PGIV; Short=CI-PGIV; AltName: Full=NADH-ubiquinone oxidoreductase 19 kDa subunit; P51970
(NDUA8_HUMAN)
2D-000NWM
7.23
15'636
{Mi}
-
1
NDUFV2 RecName: Full=NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; EC=1.6.5.3; EC=1.6.99.3; AltName: Full=NADH-ubiquinone oxidoreductase 24 kDa subunit; Flags: Precursor; P19404
(NDUV2_HUMAN)
2D-000NRJ
5.81
23'961
{Mi,Aa}
-
1
P4HB RecName: Full=Protein disulfide-isomerase; Short=PDI; EC=5.3.4.1; AltName: Full=Cellular thyroid hormone-binding protein; AltName: Full=Prolyl 4-hydroxylase subunit beta; AltName: Full=p55; Flags: Precursor; P07237
(PDIA1_HUMAN)
2D-000MV6
4.76
54'532
{Gm,Aa}
-
1
PDIA3 RecName: Full=Protein disulfide-isomerase A3; EC=5.3.4.1; AltName: Full=58 kDa glucose-regulated protein; AltName: Full=58 kDa microsomal protein; Short=p58; AltName: Full=Disulfide isomerase ER-60; AltName: Full=Endoplasmic reticulum resident protein 57; Short=ER protein 57; Short=ERp57; AltName: Full=Endoplasmic reticulum resident protein 60; Short=ER protein 60; Short=ERp60; Flags: Precursor; P30101
(PDIA3_HUMAN)
2D-000MX8
2D-000MX1
2D-000MXB
5.36
5.44
5.37
52'082
52'322
52'202
{Gm}
{Gm}
{Gm}
-
-
-
1
1
1
RPLP2 RecName: Full=60S acidic ribosomal protein P2; AltName: Full=Renal carcinoma antigen NY-REN-44; P05387
(RLA2_HUMAN)
2D-000NY7
4.40
14'034
{Gm}
-
1
SOD2 RecName: Full=Superoxide dismutase [Mn], mitochondrial; EC=1.15.1.1; Flags: Precursor; P04179
(SODM_HUMAN)
2D-000NTZ
2D-000NTY
6.86
6.73
22'283
22'324
{Mi,Aa}
{Mi,Aa}
-
-
1
1
TPI1 RecName: Full=Triosephosphate isomerase; Short=TIM; EC=5.3.1.1; AltName: Full=Triose-phosphate isomerase; P60174
(TPIS_HUMAN)
2D-000NRL
2D-000NRM
6.75
6.84
23'961
24'005
{Gm,Aa}
{Gm,Aa}
-
-
1
1
TXN RecName: Full=Thioredoxin; Short=Trx; AltName: Full=ATL-derived factor; Short=ADF; AltName: Full=Surface-associated sulphydryl protein; Short=SASP; P10599
(THIO_HUMAN)
2D-000O1N
4.88
11'271
{Gm,Aa}
-
1
UBC RecName: Full=Polyubiquitin-C; Contains: RecName: Full=Ubiquitin; Flags: Precursor; P0CG48
(UBC_HUMAN)
2D-000O34
7.18
9'474
{Gm}
-
1
UQCRFS1 RecName: Full=Cytochrome b-c1 complex subunit Rieske, mitochondrial; EC=1.10.2.2; AltName: Full=Complex III subunit 5; AltName: Full=Cytochrome b-c1 complex subunit 5; AltName: Full=Rieske iron-sulfur protein; Short=RISP; AltName: Full=Ubiquinol-cytochrome c reductase iron-sulfur subunit; Contains: RecName: Full=Cytochrome b-c1 complex subunit 11; AltName: Full=Complex III subunit IX; AltName: Full=Ubiquinol-cytochrome c reductase 8 kDa protein; Flags: Precursor; P47985
(UCRI_HUMAN)
2D-000NS8
6.52
23'563
{Mi}
-
1
Mapping legends: { Aa } => 'Amino acid composition', { Gm } => 'Gel matching', { Im } => 'Immunobloting', { Mi } => 'Microsequencing', { PMF } => 'Peptide mass fingerprinting'



Cited references

# PubMed/Medline Authors Location
[1] 9150947 Sarto C., Marocchi A., Sanchez J.-C., Giannone D., Frutiger S., Golaz O., Wilkins M.R., Doro G., Cappellano F., Hughes G.J., Hochstrasser D.F., Mocarelli P.; Electrophoresis 18(1):599-604(1997).
''''''Renal cell carcinoma and normal kidney protein expression'';'';'';
[2] 10608715 Sarto C., Frutiger S., Cappellano F., Sanchez J.-C., Doro G., Catanzaro F., Hughes G.J., Hochstrasser D.F., Mocarelli P.; Electrophoresis 20(1):3458-3466(1999).
''''''Modified expression of plasma glutathione peroidase and manganese superoxide dismutase in human renal cell carcinoma'';'';'';


Gel experimental information

Information Type Data
Map name KIDNEY_HUMAN {Kidney}
Dimension 2-D
Related documents Sample Preparation / Gel Related Documents
species Homo sapiens (Human) / TaxID:9606
Tissue (Swiss-Prot definition) Kidney
pI start: 3.50 - end: 10.00
Mw start: 10000 - end: 200000
Number of detected spots 2896
Number of identified spots 44
Number of identified proteins 28
Reference Bibliographic reference
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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server