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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P00367




SWISS-2DPAGE:  P00367


P00367


General information about the entry
View entry in simple text format
Entry nameDHE3_HUMAN
Primary accession numberP00367
integrated into SWISS-2DPAGE on August 1, 1993 (release 0)
2D Annotations were last modified onOctober 1, 2001 (version 3)
General Annotations were last modified on May 19, 2011 (version 14)
Name and origin of the protein
DescriptionRecName: Full=Glutamate dehydrogenase 1, mitochondrial; Short=GDH 1; EC=1.4.1.3; Flags: Precursor;.
Gene nameName=GLUD1
Synonyms=GLUD
Annotated speciesHomo sapiens (Human) [TaxID: 9606]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
References
[1]   MAPPING ON GEL
MEDLINE=93162045; PubMed=1286669; [NCBI, ExPASy, EBI, Israel, Japan]
Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F., Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel R.D., Hughes G.J.
''''''Human liver protein map: a reference database established by microsequencing and gel comparison'';'';''
Electrophoresis 13(1):992-1001(1992)
[2]   MAPPING ON GEL
MEDLINE=96007934; PubMed=7498157; [NCBI, ExPASy, EBI, Israel, Japan]
Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch A., Hochstrasser D.F.
''''''Inside SWISS-2DPAGE database'';'';''
Electrophoresis 16(1):1131-1151(1995)
[3]   MAPPING ON GEL
MEDLINE=97295305; PubMed=9150947; [NCBI, ExPASy, EBI, Israel, Japan]
Sarto C., Marocchi A., Sanchez J.-C., Giannone D., Frutiger S., Golaz O., Wilkins M.R., Doro G., Cappellano F., Hughes G.J., Hochstrasser D.F., Mocarelli P.
''''''Renal cell carcinoma and normal kidney protein expression'';'';''
Electrophoresis 18(1):599-604(1997)
[4]   MAPPING ON GEL
MEDLINE=98163346; PubMed=9504818; [NCBI, ExPASy, EBI, Israel, Japan]
Reymond M.A., Sanchez J.-C., Hughes G.J., Riese J., Tortola S., Peinado M.A., Kirchner T., Hohenberger W., Hochstrasser D.F., Kockerling F.
''''''Standardized characterization of gene expression in human colorectal epithelium by two-dimensional electrophoresis'';'';''
Electrophoresis 18(1):2842-2848(1997)
Comments
  • SUBUNIT: HOMOHEXAMER
2D PAGE maps for identified proteins
How to interpret a protein

CEC_HUMAN {Colorectal epithelia cells}
Homo sapiens (Human)
Tissue: Colon epithelium
CEC_HUMAN
  map experimental info
  protein estimated location
 
CEC_HUMAN

MAP LOCATIONS:
pI=7.28; Mw=53020
pI=6.51; Mw=53020
pI=6.92; Mw=52912

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [4].



HEPG2_HUMAN {HepG2}
Homo sapiens (Human)
Tissue: Hepatoblastoma
HEPG2_HUMAN
  map experimental info
  protein estimated location
 
HEPG2_HUMAN

MAP LOCATIONS:
pI=6.90; Mw=50151
pI=6.97; Mw=50011
pI=7.34; Mw=49872

MAPPING (identification):
MICROSEQUENCING AND MATCHING WITH THE LIVER MASTER GEL [2].



KIDNEY_HUMAN {Kidney}
Homo sapiens (Human)
Tissue: Kidney
KIDNEY_HUMAN
  map experimental info
  protein estimated location
 
KIDNEY_HUMAN

MAP LOCATIONS:
pI=6.81; Mw=48500
pI=6.72; Mw=48612

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [3].



LIVER_HUMAN {Liver}
Homo sapiens (Human)
Tissue: Liver
LIVER_HUMAN
  map experimental info
  protein estimated location
 
LIVER_HUMAN

MAP LOCATIONS:
pI=6.76; Mw=50483
pI=6.87; Mw=50205

MAPPING (identification):
MICROSEQUENCING [1].



U937_HUMAN {Macrophage Like Cell Line}
Homo sapiens (Human)
Tissue: Histiocytic lymphoma
U937_HUMAN
  map experimental info
  protein estimated location
 
U937_HUMAN

MAP LOCATIONS:
pI=6.84; Mw=48637

MAPPING (identification):
MATCHING WITH THE LIVER MASTER GEL [2].

Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from license@isb-sib.ch).
Cross-references
REPRODUCTION-2DPAGEIPI00016801; IPI00016801.
Siena-2DPAGEP00367; DHE3_HUMAN.
UCD-2DPAGEP00367; DHE3_HUMAN.
UniProtKB/Swiss-ProtP00367; DHE3_HUMAN.
2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameDHE3_HUMAN
Primary accession numberP00367
Secondary accession number(s) Q5TBU3
Sequence was last modified on January 1, 1990 (version 2)
Annotations were last modified on October 19, 2011 (version 140)
Name and origin of the protein
DescriptionRecName: Full=Glutamate dehydrogenase 1, mitochondrial; Short=GDH 1; EC=1.4.1.3; Flags: Precursor;
Gene nameName=GLUD1
Synonyms=GLUD
Encoded onName=GLUD1; Synonyms=GLUD
Keywords3D-structure; Acetylation; ATP-binding; Complete proteome; Direct protein sequencing; Disease mutation; GTP-binding; Mitochondrion; NADP; Nucleotide-binding; Oxidoreductase; Phosphoprotein; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX07674; CAA30521.1; -; mRNA
EMBLM20867; AAA52526.1; -; mRNA
EMBLM37154; AAA52525.1; -; mRNA
EMBLX07769; CAA30598.1; -; mRNA
EMBLJ03248; AAA52523.1; -; mRNA
EMBLX66300; CAA46994.2; -; Genomic_DNA
EMBLX66301; CAA46994.2; JOINED; Genomic_DNA
EMBLX66302; CAA46994.2; JOINED; Genomic_DNA
EMBLX66303; CAA46994.2; JOINED; Genomic_DNA
EMBLX66304; CAA46994.2; JOINED; Genomic_DNA
EMBLX66305; CAA46994.2; JOINED; Genomic_DNA
EMBLX66306; CAA46994.2; JOINED; Genomic_DNA
EMBLX66307; CAA46994.2; JOINED; Genomic_DNA
EMBLX66308; CAA46994.2; JOINED; Genomic_DNA
EMBLX66309; CAA46994.2; JOINED; Genomic_DNA
EMBLX66311; CAA46994.2; JOINED; Genomic_DNA
EMBLX66312; CAA46994.2; JOINED; Genomic_DNA
EMBLAL136982; CAI17120.1; -; Genomic_DNA
EMBLBC040132; AAH40132.1; -; mRNA
EMBLBC112946; AAI12947.1; -; mRNA
EMBLX67491; CAA47830.1; -; Genomic_DNA
IPIIPI00016801; -; .
PIRA28208; DEHUE; .
PIRI37424; I37424; .
PIRS29331; S29331; .
PIRS60192; S60192; .
RefSeqNP_005262.1; NM_005271.3; .
UniGeneHs.500409; -; .
PDB1L1F; X-ray; 2.70 A; A/B/C/D/E/F=54-558
PDB1NR1; X-ray; 3.30 A; A/B/C/D/E/F=63-558
PDBsum1L1F; -; .
PDBsum1NR1; -; .
ProteinModelPortalP00367; -; .
SMRP00367; 63-558; .
IntActP00367; 3; .
MINTMINT-5005913; -; .
STRINGP00367; -; .
PhosphoSiteP00367; -; .
SWISS-2DPAGEP00367; -; .
REPRODUCTION-2DPAGEIPI00016801; -; .
Siena-2DPAGEP00367; -; .
UCD-2DPAGEP00367; -; .
PeptideAtlasP00367; -; .
PRIDEP00367; -; .
EnsemblENST00000277865; ENSP00000277865; ENSG00000148672; .
GeneID2746; -; .
KEGGhsa:2746; -; .
NMPDRfig|9606.3.peg.4285; -; .
UCSCuc001keh.1; human; .
CTD2746; -; .
GeneCardsGC10M082663; -; .
H-InvDBHIX0009003; -; .
HGNCHGNC:4335; GLUD1; .
MIM138130; gene; .
MIM606762; phenotype; .
neXtProtNX_P00367; -; .
Orphanet35878; Hyperinsulinism-hyperammonemia syndrome; .
PharmGKBPA28737; -; .
eggNOGprNOG10069; -; .
HOGENOMHBG590661; -; .
HOVERGENHBG005479; -; .
InParanoidP00367; -; .
OMACIGVIEH; -; .
OrthoDBEOG4H72B7; -; .
PhylomeDBP00367; -; .
BioCycMetaCyc:MONOMER-11464; -; .
BRENDA1.4.1.3; 2681; .
ReactomeREACT_13; Metabolism of amino acids and derivatives; .
DrugBankDB00142; L-Glutamic Acid; .
DrugBankDB00157; NADH; .
NextBio10824; -; .
ArrayExpressP00367; -; .
BgeeP00367; -; .
CleanExHS_GLUD1; -; .
GenevestigatorP00367; -; .
GermOnlineENSG00000148672; Homo sapiens; .
GOGO:0005759; C:mitochondrial matrix; TAS:Reactome; .
GOGO:0043531; F:ADP binding; IDA:BHF-UCL; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0004352; F:glutamate dehydrogenase (NAD+) activity; IDA:UniProtKB; .
GOGO:0004353; F:glutamate dehydrogenase [NAD(P)+] activity; IDA:BHF-UCL; .
GOGO:0005525; F:GTP binding; IDA:BHF-UCL; .
GOGO:0042802; F:identical protein binding; TAS:BHF-UCL; .
GOGO:0070728; F:leucine binding; IDA:BHF-UCL; .
GOGO:0070403; F:NAD+ binding; IDA:BHF-UCL; .
GOGO:0006537; P:glutamate biosynthetic process; IDA:BHF-UCL; .
GOGO:0006538; P:glutamate catabolic process; IDA:UniProtKB; .
GOGO:0032024; P:positive regulation of insulin secretion; IMP:BHF-UCL; .
InterProIPR006095; Glu/Leu/Phe/Val_DH; .
InterProIPR006096; Glu/Leu/Phe/Val_DH_C; .
InterProIPR006097; Glu/Leu/Phe/Val_DH_dimer_dom; .
InterProIPR016040; NAD(P)-bd_dom; .
Gene3DG3DSA:3.40.50.720; NAD(P)-bd; 1; .
PfamPF00208; ELFV_dehydrog; 1; .
PfamPF02812; ELFV_dehydrog_N; 1; .
PRINTSPR00082; GLFDHDRGNASE; .
SMARTSM00839; ELFV_dehydrog; 1; .
PROSITEPS00074; GLFV_DEHYDROGENASE; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server