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Searching in 'World-2DPAGE Repository [0019]' for entry matching: ACON_RAT




World-2DPAGE Repository (0019):  ACON_RAT

ACON_RAT


General information about the entry
View entry in simple text format
Entry nameACON_RAT
Primary accession numberQ9ER34
integrated into World-2DPAGE Repository (0019) on December 9, 2009 (release 1)
2D Annotations were last modified onMay 30, 2011 (version 2)
General Annotations were last modified on January 13, 2012 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; EC=4.2.1.3; AltName: Full=Citrate hydro-lyase; Flags: Precursor;.
Gene nameName=Aco2
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1111/j.1471-4159.2010.06719.x;
VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M.
''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome''
J of Neurochemistry 113(6):1577-1588 (2010)
2D PAGE maps for identified proteins
How to interpret a protein

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus
HIPP_SYNAP
  map experimental info 		 
HIPP_SYNAP

MAP LOCATIONS:
pI=7.87; Mw=86121  [identification data]
pI=7.87; Mw=86121  [identification data]
pI=7.87; Mw=86121  [identification data]
pI=7.87; Mw=86121  [identification data]
pI=7.87; Mw=86121  [identification data]
pI=7.87; Mw=86162  [identification data]
pI=7.87; Mw=86121  [identification data]

IDENTIFICATION: SPOT 211: Peptides number=13. SeqCov=17.6%. MOWSE score=1.72E+11 [1]
SPOT 212: Peptides number=12. SeqCov=23.1%. MOWSE score=3.58E+12 [1]; SPOT 213: Peptides number=16. SeqCov=21.8%. MOWSE score=6.68E+12 [1]; SPOT 214: Peptides number=15. SeqCov=19.6%. MOWSE score=6.68E+12 [1]; SPOT 215: Peptides number=18. SeqCov=32.2%. MOWSE score=3.94E+17 [1]; SPOT 217: Peptides number=4. SeqCov=6.7%. MOWSE score=2.14E+4 [1]; SPOT 1132: Peptides number=15. SeqCov=20.4%. MOWSE score=1.03E+13 [1].
MAPPING (identification):
SPOT 211: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 212: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 213: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 214: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 215: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 217: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 1132: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Willard Freeman, Penn State College of Medicine, USA
Cross-references
UniProtKB/Swiss-ProtQ9ER34; ACON_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry nameACON_RAT
Primary accession numberQ9ER34
Secondary accession number(s) Q6P6V3
Sequence was last modified on February 1, 2005 (version 2)
Annotations were last modified on November 16, 2011 (version 77)
Name and origin of the protein
DescriptionRecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; EC=4.2.1.3; AltName: Full=Citrate hydro-lyase; Flags: Precursor;
Gene nameName=Aco2
Encoded onName=Aco2
Keywords4Fe-4S; Acetylation; Complete proteome; Direct protein sequencing; Iron; Iron-sulfur; Isopeptide bond; Lyase; Metal-binding; Mitochondrion; Pyrrolidone carboxylic acid; Reference proteome; Transit peptide; Tricarboxylic acid cycle; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAJ243266; CAC11018.1; -; mRNA
EMBLBC061999; AAH61999.1; -; mRNA
IPIIPI00421539; -; .
RefSeqNP_077374.2; NM_024398.2; .
UniGeneRn.43737; -; .
ProteinModelPortalQ9ER34; -; .
SMRQ9ER34; 29-780; .
IntActQ9ER34; 1; .
STRINGQ9ER34; -; .
PhosphoSiteQ9ER34; -; .
World-2DPAGE0004:Q9ER34; -; .
PRIDEQ9ER34; -; .
EnsemblENSRNOT00000038612; ENSRNOP00000029144; ENSRNOG00000024128; .
GeneID79250; -; .
KEGGrno:79250; -; .
NMPDRfig|10116.3.peg.27460; -; .
UCSCNM_024398; rat; .
CTD50; -; .
RGD621360; Aco2; .
eggNOGroNOG05633; -; .
GeneTreeENSGT00530000063060; -; .
HOVERGENHBG000248; -; .
InParanoidQ9ER34; -; .
OMAKHGIRWV; -; .
OrthoDBEOG4BCDM9; -; .
PhylomeDBQ9ER34; -; .
NextBio614724; -; .
ArrayExpressQ9ER34; -; .
GenevestigatorQ9ER34; -; .
GermOnlineENSRNOG00000024128; Rattus norvegicus; .
GOGO:0005739; C:mitochondrion; IDA:RGD; .
GOGO:0051538; F:3 iron; 4 sulfur cluster binding; IDA:RGD
GOGO:0051539; F:4 iron; 4 sulfur cluster binding; IDA:RGD
GOGO:0003994; F:aconitate hydratase activity; IDA:RGD; .
GOGO:0052632; F:citrate hydro-lyase (cis-aconitate-forming) activity; IEA:EC; .
GOGO:0052633; F:isocitrate hydro-lyase (cis-aconitate-forming) activity; IEA:EC; .
GOGO:0005515; F:protein binding; IPI:RGD; .
GOGO:0006101; P:citrate metabolic process; IDA:RGD; .
GOGO:0006102; P:isocitrate metabolic process; IDA:RGD; .
GOGO:0006099; P:tricarboxylic acid cycle; IDA:RGD; .
InterProIPR015931; Acnase/IPM_dHydase_lsu_aba_1/3; .
InterProIPR015937; Acoase/IPM_deHydtase; .
InterProIPR001030; Acoase/IPM_deHydtase_lsu_aba; .
InterProIPR015928; Aconitase/3IPM_dehydase_swvl; .
InterProIPR015932; Aconitase/IPMdHydase_lsu_aba_2; .
InterProIPR018136; Aconitase_4Fe-4S_BS; .
InterProIPR006248; Aconitase_mito-like; .
InterProIPR000573; AconitaseA/IPMdHydase_ssu_swvl; .
Gene3DG3DSA:3.30.499.10; Acnase/IPM_dHydase_lsu_aba_1/3; 2; .
Gene3DG3DSA:3.20.19.10; Aconitase/3IPM_dehydase_swvl; 1; .
Gene3DG3DSA:3.40.1060.10; Aconitase/IPMdHydase_lsu_aba_2; 1; .
KOK01681; -; .
PANTHERPTHR11670; Aconitase-like_core; 1; .
PANTHERPTHR11670:SF5; Aconitase_mito; 1; .
PfamPF00330; Aconitase; 1; .
PfamPF00694; Aconitase_C; 1; .
PRINTSPR00415; ACONITASE; .
SUPFAMSSF52016; Aconitase/3IPM_dehydase_swvl; 1; .
SUPFAMSSF53732; Aconitase_N; 1; .
TIGRFAMsTIGR01340; Aconitase_mito; 1; .
PROSITEPS00450; ACONITASE_1; 1; .
PROSITEPS01244; ACONITASE_2; 1; .


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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server