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Searching in 'World-2DPAGE Repository [0022]' for entry matching: GRP75_RAT




World-2DPAGE Repository (0022):  GRP75_RAT


GRP75_RAT


General information about the entry
View entry in simple text format
Entry nameGRP75_RAT
Primary accession numberP48721
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Stress-70 protein, mitochondrial; AltName: Full=75 kDa glucose-regulated protein; Short=GRP-75; AltName: Full=Heat shock 70 kDa protein 9; AltName: Full=Mortalin; AltName: Full=Peptide-binding protein 74; Short=PBP74; AltName: Full=mtHSP70; Flags: Precursor;.
Gene nameName=Hspa9
Synonyms=Grp75, Hspa9a
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info
 
HCR_LCR_HEART

MAP LOCATIONS:
pI=5.97; Mw=74097  [identification data]
pI=5.97; Mw=74097  [identification data]

IDENTIFICATION: SPOT 785: SeqCov=20%. Peptides MS=12/20. Mascot PMF Score=84 [1]
SPOT 790: SeqCov=11.9%. Peptides MS=7/20. Mascot PMF Score=33 [1].
PEPTIDE EVIDENCE: SPOT 785: 378-SDIGEVILVGGMTR-391 (104) / 378-SDIGEVILVGGMTR-391 Oxidation (M) (48) / 499-LLGQFTLIGIPPAPR-513 (70) [1]
SPOT 790: 378-SDIGEVILVGGMTR-391 (81) / 378-SDIGEVILVGGMTR-391 Oxidation (M) (37) / 499-LLGQFTLIGIPPAPR-513 (57) [1].
MAPPING (identification):
SPOT 785: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 790: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtP48721; GRP75_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameGRP75_RAT
Primary accession numberP48721
Sequence was last modified on October 17, 2006 (version 3)
Annotations were last modified on November 16, 2011 (version 95)
Name and origin of the protein
DescriptionRecName: Full=Stress-70 protein, mitochondrial; AltName: Full=75 kDa glucose-regulated protein; Short=GRP-75; AltName: Full=Heat shock 70 kDa protein 9; AltName: Full=Mortalin; AltName: Full=Peptide-binding protein 74; Short=PBP74; AltName: Full=mtHSP70; Flags: Precursor;
Gene nameName=Hspa9
Synonyms=Grp75, Hspa9a
Encoded onName=Hspa9; Synonyms=Grp75, Hspa9a
KeywordsAcetylation; ATP-binding; Chaperone; Complete proteome; Direct protein sequencing; Mitochondrion; Nucleotide-binding; Phosphoprotein; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLS75280; AAB33049.1; -; mRNA
EMBLS78556; AAB34982.1; -; mRNA
IPIIPI00363265; -; .
PIRI56581; I56581; .
RefSeqNP_001094128.1; NM_001100658.1; .
UniGeneRn.7535; -; .
ProteinModelPortalP48721; -; .
SMRP48721; 55-580; .
IntActP48721; 2; .
MINTMINT-4592308; -; .
STRINGP48721; -; .
PhosphoSiteP48721; -; .
World-2DPAGE0004:P48721; -; .
PRIDEP48721; -; .
GeneID291671; -; .
KEGGrno:291671; -; .
UCSCS78556; rat; .
CTD3313; -; .
RGD1311806; Hspa9; .
eggNOGmaNOG05443; -; .
GeneTreeENSGT00550000074813; -; .
HOVERGENHBG051845; -; .
InParanoidP48721; -; .
OrthoDBEOG49KFQ6; -; .
PhylomeDBP48721; -; .
NextBio632983; -; .
ArrayExpressP48721; -; .
GenevestigatorP48721; -; .
GermOnlineENSRNOG00000019525; Rattus norvegicus; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0051082; F:unfolded protein binding; IEA:InterPro; .
GOGO:0006457; P:protein folding; IEA:InterPro; .
InterProIPR012725; Chaperone_DnaK; .
InterProIPR018181; Heat_shock_70_CS; .
InterProIPR001023; Hsp70; .
InterProIPR013126; Hsp_70; .
KOK04043; -; .
PANTHERPTHR19375; Hsp70; 1; .
PfamPF00012; HSP70; 1; .
PRINTSPR00301; HEATSHOCK70; .
TIGRFAMsTIGR02350; Prok_dnaK; 1; .
PROSITEPS00297; HSP70_1; 1; .
PROSITEPS00329; HSP70_2; 1; .
PROSITEPS01036; HSP70_3; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server