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Searching in 'World-2DPAGE Repository [0021]' for entry matching: KBL_MOUSE




World-2DPAGE Repository (0021):  KBL_MOUSE


KBL_MOUSE


General information about the entry
View entry in simple text format
Entry nameKBL_MOUSE
Primary accession numberO88986
integrated into World-2DPAGE Repository (0021) on June 9, 2010 (release 1)
2D Annotations were last modified onJune 20, 2011 (version 2)
General Annotations were last modified on November 30, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial; Short=AKB ligase; EC=2.3.1.29; AltName: Full=Aminoacetone synthase; AltName: Full=Glycine acetyltransferase; Flags: Precursor;.
Gene nameName=Gcat
Synonyms=Kbl
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
De Cegli R., Romito A., Iacobacci S., Mao L., Lauria M., Fedele A.O., Klose J., Borel C., Descombes P., Antonarakis S.E., Di Bernardo D., Banfi S., Ballabio A., Cobellis G.
''A mouse Embryonic Stem Cell Bank for inducible overexpression of human Chromosome 21 genes''
Genome Biol 11(6):R64 (2010)
2D PAGE maps for identified proteins
How to interpret a protein

RUNX1_DAY7 {Mouse embryonic stem cells overexpressing Runx1, Day 7}
Mus musculus (Mouse)
Tissue: Embryonic stem cell
RUNX1_DAY7
  map experimental info
 
RUNX1_DAY7

MAP LOCATIONS:
pI=6.92; Mw=44900  [identification data]

EXPRESSION:
SPOT 631: Protein ratio 48h/0h=1.29 [1].

IDENTIFICATION: SPOT 631: Mascot Protein score=114. SeqCov=5%. Peptides identified=2 [1].
MAPPING (identification):
SPOT 631: Tandem mass spectrometry [1].

Copyright
Data from Dr. Lei Mao, Institute of Human Genetics, Charite Universitaetsmedizin Berlin, Germany
Cross-references
UniProtKB/Swiss-ProtO88986; KBL_MOUSE.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameKBL_MOUSE
Primary accession numberO88986
Sequence was last modified on January 1, 1999 (version 2)
Annotations were last modified on November 16, 2011 (version 96)
Name and origin of the protein
DescriptionRecName: Full=2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial; Short=AKB ligase; EC=2.3.1.29; AltName: Full=Aminoacetone synthase; AltName: Full=Glycine acetyltransferase; Flags: Precursor;
Gene nameName=Gcat
Synonyms=Kbl
Encoded onName=Gcat; Synonyms=Kbl
KeywordsAcyltransferase; Complete proteome; Mitochondrion; Pyridoxal phosphate; Reference proteome; Transferase; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAF093403; AAC99774.1; -; mRNA
EMBLAK087433; BAC39872.1; -; mRNA
EMBLBC024107; AAH24107.1; -; mRNA
IPIIPI00136655; -; .
RefSeqNP_038875.3; NM_013847.4; .
UniGeneMm.237085; -; .
ProteinModelPortalO88986; -; .
SMRO88986; 23-415; .
STRINGO88986; -; .
PhosphoSiteO88986; -; .
SWISS-2DPAGEO88986; -; .
REPRODUCTION-2DPAGEO88986; -; .
PRIDEO88986; -; .
EnsemblENSMUST00000006544; ENSMUSP00000006544; ENSMUSG00000006378; .
GeneID26912; -; .
KEGGmmu:26912; -; .
UCSCuc007wse.2; mouse; .
CTD23464; -; .
MGIMGI:1349389; Gcat; .
eggNOGroNOG06498; -; .
HOGENOMHBG649839; -; .
HOVERGENHBG105208; -; .
InParanoidO88986; -; .
OMAVPPNSCR; -; .
OrthoDBEOG4BCDMZ; -; .
PhylomeDBO88986; -; .
ArrayExpressO88986; -; .
BgeeO88986; -; .
GenevestigatorO88986; -; .
GermOnlineENSMUSG00000006378; Mus musculus; .
GOGO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell; .
GOGO:0008890; F:glycine C-acetyltransferase activity; IEA:EC; .
GOGO:0030170; F:pyridoxal phosphate binding; IEA:InterPro; .
GOGO:0016769; F:transferase activity; transferring nitrogenous groups; IEA:InterPro
GOGO:0009058; P:biosynthetic process; IEA:InterPro; .
InterProIPR011282; 2am3keto_CoA_ligase; .
InterProIPR001917; Aminotrans_II_pyridoxalP_BS; .
InterProIPR004839; Aminotransferase_I/II; .
InterProIPR015424; PyrdxlP-dep_Trfase_major_dom; .
InterProIPR015421; PyrdxlP-dep_Trfase_major_sub1; .
InterProIPR015422; PyrdxlP-dep_Trfase_major_sub2; .
Gene3DG3DSA:3.40.640.10; PyrdxlP-dep_Trfase_major_sub1; 1; .
Gene3DG3DSA:3.90.1150.10; PyrdxlP-dep_Trfase_major_sub2; 1; .
KOK00639; -; .
PfamPF00155; Aminotran_1_2; 1; .
SUPFAMSSF53383; PyrdxlP-dep_Trfase_major; 1; .
TIGRFAMsTIGR01822; 2am3keto_CoA; 1; .
PROSITEPS00599; AA_TRANSFER_CLASS_2; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server