Attention: World-2DPAGE is no longer maintained.

RecName: Full=Pyruvate kinase PKM; EC=2.7.1.40; AltName: Full=Cytosolic thyroid hormone-binding protein; Short=CTHBP; AltName: Full=Opa-interacting protein 3; Short=OIP-3; AltName: Full=Pyruvate kinase 2/3; AltName: Full=Pyruvate kinase muscle isozyme; AltName: Full=Thyroid hormone-binding protein 1; Short=THBP1; AltName: Full=Tumor M2-PK; AltName: Full=p58;.

Gene name

Name=PKM
Synonyms=OIP3, PK2, PK3, PKM2

Annotated species

Homo sapiens (Human) [TaxID: 9606]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.

References

[1]

2D GEL CHARACTERIZATION
DOI=10.1074/mcp.M112.022947;
Vernocchi S., Battello N., Schmitz S., Revets D., Billing A.M., Turner J.D., Muller C.P.
''Membrane glucocorticoid receptor activation induces proteomic changes aligning with classical glucocorticoid-effects''
Molecular & Cellular Proteomics 12(7):1764-1779 (2013)

2D PAGE maps for identified proteins

How to interpret a protein

HSAPIENS_CCRF-CEM_3-10 {CCRF-CEM cells stimulated with Cort-BSA (internal standard)}
Homo sapiens (Human)

map experimental info

HSAPIENS_CCRF-CEM_3-10

MAP LOCATIONS:

SPOT 634:

pI=7.95; Mw=57806

[identification data]

%COV: SPOT 634: 65 [1].
SCORE: SPOT 634: 803 [1].
SEARCH ENGINE: SPOT 634: Mascot [1].
MAPPING (identification):

SPOT 634: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Claude P. Muller, Centre de Recherche Public de la Sante / National Public Health Laboratory, Luxembourg

Cross-references

UniProtKB/Swiss-Prot

P14618; KPYM_HUMAN.

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 0.0
Entry name	KPYM_HUMAN
Primary accession number	P14618
Secondary accession number(s)	A6NFK3 B2R5N8 B3KRY0 B4DFX8 B4DUU6 P14786 Q53GK4 Q96E76 Q9BWB5 Q9UCV6 Q9UPF2
Sequence was last modified on	January 23, 2007 (version 4)
Annotations were last modified on	October 1, 2014 (version 195)
Name and origin of the protein
Description	RecName: Full=Pyruvate kinase PKM; EC=2.7.1.40; AltName: Full=Cytosolic thyroid hormone-binding protein; Short=CTHBP; AltName: Full=Opa-interacting protein 3; Short=OIP-3; AltName: Full=Pyruvate kinase 2/3; AltName: Full=Pyruvate kinase muscle isozyme; AltName: Full=Thyroid hormone-binding protein 1; Short=THBP1; AltName: Full=Tumor M2-PK; AltName: Full=p58;
Gene name	Name=PKM Synonyms=OIP3, PK2, PK3, PKM2
Encoded on	Name=PKM; Synonyms=OIP3, PK2, PK3, PKM2
Keywords	3D-structure; Acetylation; Allosteric enzyme; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Hydroxylation; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Potassium; Pyruvate; Reference proteome; Transferase; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	M23725; AAA36449.1; -; mRNA
EMBL	M26252; AAA36672.1; -; mRNA
EMBL	X56494; CAA39849.1; -; Genomic_DNA
EMBL	AK092369; BAG52542.1; -; mRNA
EMBL	AK222927; BAD96647.1; -; mRNA
EMBL	AK294315; BAG57589.1; ALT_INIT; mRNA
EMBL	AK300800; BAG62458.1; -; mRNA
EMBL	AK312253; BAG35185.1; -; mRNA
EMBL	AY352517; AAQ15274.1; -; Genomic_DNA
EMBL	AC020779; -; NOT_ANNOTATED_CDS; Genomic_DNA
EMBL	CH471082; EAW77884.1; -; Genomic_DNA
EMBL	CH471082; EAW77888.1; -; Genomic_DNA
EMBL	BC000481; AAH00481.3; -; mRNA
EMBL	BC007640; AAH07640.1; -; mRNA
EMBL	BC007952; AAH07952.3; -; mRNA
EMBL	BC012811; AAH12811.3; -; mRNA
EMBL	BC035198; AAH35198.1; -; mRNA
EMBL	AF025439; AAC39559.1; -; mRNA
CCDS	CCDS32284.1; -. [P14618-1]; .
CCDS	CCDS32285.1; -. [P14618-2]; .
CCDS	CCDS55972.1; -. [P14618-3]; .
PIR	S30038; S30038; .
PIR	S64635; S64635; .
RefSeq	NP_001193725.1; NM_001206796.1; .
RefSeq	NP_001193726.1; NM_001206797.1; .
RefSeq	NP_001193727.1; NM_001206798.1. [P14618-3]; .
RefSeq	NP_001193728.1; NM_001206799.1; .
RefSeq	NP_002645.3; NM_002654.4. [P14618-1]; .
RefSeq	NP_872270.1; NM_182470.2. [P14618-2]; .
RefSeq	NP_872271.1; NM_182471.2. [P14618-2]; .
RefSeq	XP_005254502.1; XM_005254445.2. [P14618-1]; .
UniGene	Hs.534770; -; .
PDB	1T5A; X-ray; 2.80 A; A/B/C/D=1-531
PDB	1ZJH; X-ray; 2.20 A; A=3-531
PDB	3BJF; X-ray; 2.03 A; A/B/C/D=14-531
PDB	3BJT; X-ray; 2.50 A; A/B/C/D=2-531
PDB	3G2G; X-ray; 2.00 A; A/B/C/D=1-531
PDB	3GQY; X-ray; 1.85 A; A/B/C/D=1-531
PDB	3GR4; X-ray; 1.60 A; A/B/C/D=1-531
PDB	3H6O; X-ray; 2.00 A; A/B/C/D=1-531
PDB	3ME3; X-ray; 1.95 A; A/B/C/D=1-531
PDB	3SRD; X-ray; 2.90 A; A/B/C/D=1-531
PDB	3SRF; X-ray; 2.84 A; A/B/C/D/E/F/G/H=1-531
PDB	3SRH; X-ray; 2.60 A; A/B/C/D=1-531
PDB	3U2Z; X-ray; 2.10 A; A/B/C/D=1-531
PDB	4B2D; X-ray; 2.30 A; A/B/C/D=2-531
PDB	4FXF; X-ray; 2.55 A; A/B/C/D=1-531
PDB	4FXJ; X-ray; 2.90 A; A/B/C/D=1-531
PDB	4G1N; X-ray; 2.30 A; A/B/C/D=14-531
PDB	4JPG; X-ray; 2.33 A; A/B/C/D=1-531
PDBsum	1T5A; -; .
PDBsum	1ZJH; -; .
PDBsum	3BJF; -; .
PDBsum	3BJT; -; .
PDBsum	3G2G; -; .
PDBsum	3GQY; -; .
PDBsum	3GR4; -; .
PDBsum	3H6O; -; .
PDBsum	3ME3; -; .
PDBsum	3SRD; -; .
PDBsum	3SRF; -; .
PDBsum	3SRH; -; .
PDBsum	3U2Z; -; .
PDBsum	4B2D; -; .
PDBsum	4FXF; -; .
PDBsum	4FXJ; -; .
PDBsum	4G1N; -; .
PDBsum	4JPG; -; .
ProteinModelPortal	P14618; -; .
SMR	P14618; 13-531; .
BioGrid	111332; 106; .
DIP	DIP-31273N; -; .
IntAct	P14618; 228; .
MINT	MINT-4998892; -; .
BindingDB	P14618; -; .
ChEMBL	CHEMBL1075189; -; .
DrugBank	DB00119; Pyruvic acid; .
PhosphoSite	P14618; -; .
DMDM	20178296; -; .
DOSAC-COBS-2DPAGE	P14618; -; .
OGP	P14618; -; .
REPRODUCTION-2DPAGE	IPI00220644; -; .
REPRODUCTION-2DPAGE	IPI00479186; -; .
UCD-2DPAGE	P14618; -; .
MaxQB	P14618; -; .
PaxDb	P14618; -; .
PRIDE	P14618; -; .
DNASU	5315; -; .
Ensembl	ENST00000319622; ENSP00000320171; ENSG00000067225. [P14618-2]; .
Ensembl	ENST00000335181; ENSP00000334983; ENSG00000067225. [P14618-1]; .
Ensembl	ENST00000389093; ENSP00000373745; ENSG00000067225. [P14618-2]; .
Ensembl	ENST00000449901; ENSP00000403365; ENSG00000067225. [P14618-3]; .
Ensembl	ENST00000565154; ENSP00000455901; ENSG00000067225. [P14618-2]; .
Ensembl	ENST00000565184; ENSP00000455736; ENSG00000067225. [P14618-2]; .
Ensembl	ENST00000568459; ENSP00000456970; ENSG00000067225. [P14618-2]; .
GeneID	5315; -; .
KEGG	hsa:5315; -; .
UCSC	uc002atv.2; human. [P14618-2]; .
UCSC	uc002aty.2; human. [P14618-1]; .
UCSC	uc010ukj.2; human. [P14618-3]; .
CTD	5315; -; .
GeneCards	GC15M072492; -; .
HGNC	HGNC:9021; PKM; .
HPA	CAB019421; -; .
HPA	HPA029501; -; .
MIM	179050; gene; .
neXtProt	NX_P14618; -; .
PharmGKB	PA33353; -; .
eggNOG	COG0469; -; .
HOGENOM	HOG000021559; -; .
HOVERGEN	HBG000941; -; .
InParanoid	P14618; -; .
KO	K00873; -; .
OMA	INLFVDY; -; .
OrthoDB	EOG78M01Q; -; .
PhylomeDB	P14618; -; .
TreeFam	TF300390; -; .
BioCyc	MetaCyc:HS00906-MONOMER; -; .
Reactome	REACT_1383; Glycolysis; .
SABIO-RK	P14618; -; .
UniPathway	UPA00109; UER00188; .
ChiTaRS	PKM2; human; .
EvolutionaryTrace	P14618; -; .
GeneWiki	PKM2; -; .
GenomeRNAi	5315; -; .
NextBio	20554; -; .
PRO	PR:P14618; -; .
ArrayExpress	P14618; -; .
Bgee	P14618; -; .
CleanEx	HS_PKM2; -; .
Genevestigator	P14618; -; .
GO	GO:0005929; C:cilium; IEA:Ensembl; .
GO	GO:0005737; C:cytoplasm; IDA:UniProtKB; .
GO	GO:0005829; C:cytosol; NAS:UniProtKB; .
GO	GO:0070062; C:extracellular vesicular exosome; IDA:UniProtKB; .
GO	GO:0005739; C:mitochondrion; IDA:UniProt; .
GO	GO:0005634; C:nucleus; IDA:UniProtKB; .
GO	GO:0005886; C:plasma membrane; IDA:HPA; .
GO	GO:0031982; C:vesicle; IDA:UniProtKB; .
GO	GO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GO	GO:0000287; F:magnesium ion binding; IEA:InterPro; .
GO	GO:0023026; F:MHC class II protein complex binding; IDA:UniProt; .
GO	GO:0044822; F:poly(A) RNA binding; IDA:UniProtKB; .
GO	GO:0030955; F:potassium ion binding; IEA:InterPro; .
GO	GO:0005515; F:protein binding; IPI:IntAct; .
GO	GO:0004743; F:pyruvate kinase activity; TAS:UniProtKB; .
GO	GO:0005975; P:carbohydrate metabolic process; TAS:Reactome; .
GO	GO:0006006; P:glucose metabolic process; TAS:Reactome; .
GO	GO:0006096; P:glycolytic process; TAS:Reactome; .
GO	GO:0012501; P:programmed cell death; IDA:UniProtKB; .
GO	GO:0044281; P:small molecule metabolic process; TAS:Reactome; .
Gene3D	2.40.33.10; -; 1; .
Gene3D	3.20.20.60; -; 2; .
Gene3D	3.40.1380.20; -; 1; .
InterPro	IPR001697; Pyr_Knase; .
InterPro	IPR015813; Pyrv/PenolPyrv_Kinase-like_dom; .
InterPro	IPR011037; Pyrv_Knase-like_insert_dom; .
InterPro	IPR015794; Pyrv_Knase_a/b; .
InterPro	IPR018209; Pyrv_Knase_AS; .
InterPro	IPR015793; Pyrv_Knase_brl; .
InterPro	IPR015795; Pyrv_Knase_C; .
InterPro	IPR015806; Pyrv_Knase_insert_dom; .
PANTHER	PTHR11817; PTHR11817; 1; .
Pfam	PF00224; PK; 1; .
Pfam	PF02887; PK_C; 1; .
PRINTS	PR01050; PYRUVTKNASE; .
SUPFAM	SSF50800; SSF50800; 1; .
SUPFAM	SSF51621; SSF51621; 2; .
SUPFAM	SSF52935; SSF52935; 1; .
TIGRFAMs	TIGR01064; pyruv_kin; 1; .
PROSITE	PS00110; PYRUVATE_KINASE; 1; .

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server

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