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World-2DPAGE Repository  Search by  [accession number]  [description, ID or gene]  [author names]  [spot ID / serial number]  [identification methods]  [pI / Mw range]  [combined fields]  Maps  [experimental info]  [protein list]  [graphical interface]      Databases 0001 0002 0003 0004 0005 0006 0007 0008 0009 0010 0011 0012 0014 0017 0018 0019 0020 0021 0022 0023 0024 0026 0027 0029 0030 0031 0032 0034 0035 0036 0037 0038 0039 0040 0042 0043 0044 0046 0047 0050 0051 0053 0054 0056 0059 0061 0062 0063 0066 0068 0069 0076 0077 0080 0084 0085 0088 use 'Ctrl' to select several databases Select Remote Interfaces [All Interfaces] SWISS-2DPAGE World-2DPAGE Portal Exclude local DBs has only effect if a remote interface is selected

Searching in 'World-2DPAGE Repository [0043]' for entry matching: KPYM_MOUSE World-2DPAGE Repository (0043):  KPYM_MOUSE KPYM_MOUSE General information about the entry View entry in simple text format Entry name KPYM_MOUSE Primary accession number P52480 integrated into World-2DPAGE Repository (0043) on  January 4, 2012 (release 1) 2D Annotations were last modified on January 6, 2012 (version 1) General Annotations were last modified on  April 18, 2012 (version 2) Name and origin of the protein Description RecName: Full=Pyruvate kinase isozymes M1/M2; EC=2.7.1.40; AltName: Full=Pyruvate kinase muscle isozyme;. Gene name Name=Pkm2 Synonyms=Pk3, Pykm Annotated species Mus musculus (Mouse) [TaxID: 10090] Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus. References [1]   2D GEL CHARACTERIZATION DOI=10.1016/j.jprot.2012.04.001 ; Sun Z., Biela L.M., Hamilton K.L., Reardon K.F. ''Concentration-dependent effects of the soy phytoestrogen genistein on the proteome of cultured cardiomyocytes'' Journal of Proteomics 0(0):0-0 (2012) 2D PAGE maps for identified proteins How to interpret a protein MMUSCULUS_HL-1PHI_4-7 {mouse HL-1 cultured cardiomyocytes hydrophilic extraction} Mus musculus (Mouse) Tissue: Cardiac myocyte   map experimental info   MMUSCULUS_HL-1PHI_4-7 MAP LOCATIONS: BAND phi26: Mw=58420   [identification data] BAND phi71: Mw=58420   [identification data] %COV: BAND phi26: 3.3 [1] BAND phi71: 24.3 [1]. GENEID: BAND phi26: KPYM_MOUSE [1] BAND phi71: KPYM_MOUSE [1]. SCORE: BAND phi26: 140 [1] BAND phi71: 313 [1]. SEARCH ENGINE: BAND phi26: Mascot [1] BAND phi71: Mascot [1]. MAPPING (identification): BAND phi26: Tandem mass spectrometry [1]; BAND phi71: Tandem mass spectrometry [1]. Copyright Data from Dr. Zeyu Sun, Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, USA Cross-references IPI 00407130. UniProtKB/Swiss-Prot P52480; KPYM_MOUSE. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2012_03 Entry name KPYM_MOUSE Primary accession number P52480 Secondary accession number(s) Q3TBV8 Q3TBW5 Q3TC59 Q3U1X3 Q3U5P6 Q4VC20 Q64484 Q91YI8 Q9CWB1 Sequence was last modified on  May 1, 2007 (version 4) Annotations were last modified on  March 21, 2012 (version 129) Name and origin of the protein Description RecName: Full=Pyruvate kinase isozymes M1/M2; EC=2.7.1.40; AltName: Full=Pyruvate kinase muscle isozyme; Gene name Name=Pkm2 Synonyms=Pk3, Pykm Encoded on Name=Pkm2; Synonyms=Pk3, Pykm Keywords Acetylation; Allosteric enzyme; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Hydroxylation; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Potassium; Pyruvate; Reference proteome; Transferase; Ubl conjugation. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL D38379; BAA07457.1; -; mRNA EMBL X97047; CAA65761.1; -; mRNA EMBL AK002341; BAB22025.1; -; mRNA EMBL AK135397; BAE22519.1; -; mRNA EMBL AK151724; BAE30642.1; -; mRNA EMBL AK153483; BAE32031.1; -; mRNA EMBL AK155110; BAE33055.1; -; mRNA EMBL AK155655; BAE33370.1; -; mRNA EMBL AK170892; BAE42098.1; -; mRNA EMBL AK168943; BAE40751.1; -; mRNA EMBL AK171023; BAE42192.1; -; mRNA EMBL AK171033; BAE42199.1; -; mRNA EMBL AC160637; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL BC016619; AAH16619.1; -; mRNA EMBL BC094663; AAH94663.1; -; mRNA IPI IPI00407130; -; . IPI IPI00845840; -; . PIR S55921; S55921; . RefSeq NP_001240812.1; NM_001253883.1; . RefSeq NP_035229.2; NM_011099.3; . UniGene Mm.326167; -; . UniGene Mm.405069; -; . ProteinModelPortal P52480; -; . SMR P52480; 13-531; . IntAct P52480; 8; . MINT MINT-1850796; -; . STRING P52480; -; . PhosphoSite P52480; -; . SWISS-2DPAGE P52480; -; . PMMA-2DPAGE P52480; -; . REPRODUCTION-2DPAGE IPI00407130; -; . REPRODUCTION-2DPAGE P52480; -; . PRIDE P52480; -; . Ensembl ENSMUST00000034834; ENSMUSP00000034834; ENSMUSG00000032294; . Ensembl ENSMUST00000163694; ENSMUSP00000128770; ENSMUSG00000032294; . GeneID 18746; -; . KEGG mmu:18746; -; . UCSC uc009pyf.1; mouse; . UCSC uc009pyi.1; mouse; . CTD 5315; -; . MGI MGI:97591; Pkm2; . eggNOG COG0469; -; . GeneTree ENSGT00390000008859; -; . HOVERGEN HBG000941; -; . InParanoid P52480; -; . KO K00873; -; . OMA PANEGNT; -; . OrthoDB EOG40GCQJ; -; . PhylomeDB P52480; -; . NextBio 294905; -; . ArrayExpress P52480; -; . Bgee P52480; -; . CleanEx MM_PKM2; -; . Genevestigator P52480; -; . GermOnline ENSMUSG00000032294; Mus musculus; . GO GO:0019861; C:flagellum; IDA:MGI; . GO GO:0005739; C:mitochondrion; IDA:MGI; . GO GO:0005634; C:nucleus; IEA:UniProtKB-SubCell; . GO GO:0005524; F:ATP binding; IEA:UniProtKB-KW; . GO GO:0000287; F:magnesium ion binding; IEA:InterPro; . GO GO:0030955; F:potassium ion binding; IEA:InterPro; . GO GO:0005515; F:protein binding; IPI:IntAct; . GO GO:0004743; F:pyruvate kinase activity; IDA:MGI; . GO GO:0006096; P:glycolysis; IDA:MGI; . InterPro IPR001697; Pyr_Knase; . InterPro IPR015813; Pyrv/PenolPyrv_Kinase; . InterPro IPR011037; Pyrv_Knase-like_insert_dom; . InterPro IPR015794; Pyrv_Knase_a/b; . InterPro IPR018209; Pyrv_Knase_AS; . InterPro IPR015793; Pyrv_Knase_brl; . InterPro IPR015795; Pyrv_Knase_C; . InterPro IPR015806; Pyrv_Knase_insert_dom; . Gene3D G3DSA:2.40.33.10; PK_B_barrel_like; 1; . Gene3D G3DSA:3.20.20.60; Pyrv/PenolPyrv_Kinase_cat; 2; . Gene3D G3DSA:3.40.1380.20; Pyrv_Knase_a/b; 1; . PANTHER PTHR11817; Pyruvate_kinase; 1; . Pfam PF00224; PK; 1; . Pfam PF02887; PK_C; 1; . PRINTS PR01050; PYRUVTKNASE; . SUPFAM SSF50800; PK_B_barrel_like; 1; . SUPFAM SSF52935; Pyruvate_kinase; 1; . SUPFAM SSF51621; Pyrv/PenolPyrv_Kinase_cat; 1; . TIGRFAMs TIGR01064; Pyruv_kin; 1; . PROSITE PS00110; PYRUVATE_KINASE; 1; . 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