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World-2DPAGE Repository  Search by  [accession number]  [description, ID or gene]  [author names]  [spot ID / serial number]  [identification methods]  [pI / Mw range]  [combined fields]  Maps  [experimental info]  [protein list]  [graphical interface]      Databases 0001 0002 0003 0004 0005 0006 0007 0008 0009 0010 0011 0012 0014 0017 0018 0019 0020 0021 0022 0023 0024 0026 0027 0029 0030 0031 0032 0034 0035 0036 0037 0038 0039 0040 0042 0043 0044 0046 0047 0050 0051 0053 0054 0056 0059 0061 0062 0063 0066 0068 0069 0076 0077 0080 0084 0085 0088 use 'Ctrl' to select several databases Select Remote Interfaces [All Interfaces] SWISS-2DPAGE World-2DPAGE Portal Exclude local DBs has only effect if a remote interface is selected

Searching in 'World-2DPAGE Repository [0030]' for entry matching: KPYM_RAT World-2DPAGE Repository (0030):  KPYM_RAT KPYM_RAT General information about the entry View entry in simple text format Entry name KPYM_RAT Primary accession number P11980 integrated into World-2DPAGE Repository (0030) on  November 9, 2010 (release 1) 2D Annotations were last modified on June 21, 2011 (version 2) General Annotations were last modified on  November 23, 2011 (version 2) Name and origin of the protein Description RecName: Full=Pyruvate kinase isozymes M1/M2; EC=2.7.1.40; AltName: Full=Pyruvate kinase muscle isozyme;. Gene name Name=Pkm2 Synonyms=Pykm Annotated species Rattus norvegicus (Rat) [TaxID: 10116] Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus. References [1]   2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000006; D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C. ''Two-dimensional gel proteome reference map of INS-1E cells'' Proteomics 11(7):1365-1369 (2011) 2D PAGE maps for identified proteins How to interpret a protein RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7} Rattus norvegicus (Rat)   map experimental info   RAT_INS1E_4-7 MAP LOCATIONS: SPOT 812: pI=6.77; Mw=68969   [identification data] SPOT 856: pI=6.77; Mw=67087   [identification data] SPOT 1603: pI=5.04; Mw=42675   [identification data] SPOT 1651: pI=5.00; Mw=41799   [identification data] IDENTIFICATION: SPOT 812: Mascot protein score=211. Number of peptides=4 [1] SPOT 856: Mascot protein score=137. Number of peptides=5 [1]; SPOT 1603: Mascot protein score=265. Number of peptides=5 [1]; SPOT 1651: Mascot protein score=281. Number of peptides=7 [1]. MAPPING (identification): SPOT 812: Tandem mass spectrometry [1]; SPOT 856: Tandem mass spectrometry [1]; SPOT 1603: Tandem mass spectrometry [1]; SPOT 1651: Tandem mass spectrometry [1]. Copyright Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium Cross-references UniProtKB/Swiss-Prot P11980; KPYM_RAT. World-2DPAGE Repository P11980; KPYM_RAT. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_11 Entry name KPYM_RAT Primary accession number P11980 Secondary accession number(s) P11981 Sequence was last modified on  January 23, 2007 (version 3) Annotations were last modified on  November 16, 2011 (version 113) Name and origin of the protein Description RecName: Full=Pyruvate kinase isozymes M1/M2; EC=2.7.1.40; AltName: Full=Pyruvate kinase muscle isozyme; Gene name Name=Pkm2 Synonyms=Pykm Encoded on Name=Pkm2; Synonyms=Pykm Keywords Acetylation; Allosteric enzyme; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Potassium; Pyruvate; Reference proteome; Transferase; Ubl conjugation. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL M24359; AAB93666.1; -; Genomic_DNA EMBL M24359; AAB93667.1; -; Genomic_DNA EMBL M14377; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL X15800; CAA33799.1; -; mRNA IPI IPI00231929; -; . IPI IPI00339197; -; . PIR A26186; A26186; . PIR B26186; B26186; . RefSeq NP_445749.1; NM_053297.1; . UniGene Rn.1556; -; . ProteinModelPortal P11980; -; . SMR P11980; 11-531; . IntAct P11980; 1; . MINT MINT-4587504; -; . STRING P11980; -; . PhosphoSite P11980; -; . World-2DPAGE 0004:P11980; -; . PRIDE P11980; -; . Ensembl ENSRNOT00000015332; ENSRNOP00000015331; ENSRNOG00000011329; . Ensembl ENSRNOT00000015398; ENSRNOP00000015398; ENSRNOG00000011329; . GeneID 25630; -; . KEGG rno:25630; -; . UCSC NM_053297; rat; . CTD 5315; -; . RGD 3337; Pkm2; . eggNOG maNOG12287; -; . GeneTree ENSGT00390000008859; -; . HOVERGEN HBG000941; -; . InParanoid P11980; -; . OrthoDB EOG40GCQJ; -; . PhylomeDB P11980; -; . NextBio 607431; -; . Genevestigator P11980; -; . GermOnline ENSRNOG00000011329; Rattus norvegicus; . GO GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; . GO GO:0005634; C:nucleus; IEA:UniProtKB-SubCell; . GO GO:0005625; C:soluble fraction; IDA:RGD; . GO GO:0043531; F:ADP binding; IDA:RGD; . GO GO:0005524; F:ATP binding; IEA:UniProtKB-KW; . GO GO:0000287; F:magnesium ion binding; IEA:InterPro; . GO GO:0030955; F:potassium ion binding; IEA:InterPro; . GO GO:0005515; F:protein binding; IPI:RGD; . GO GO:0004743; F:pyruvate kinase activity; IDA:RGD; . GO GO:0006754; P:ATP biosynthetic process; IDA:RGD; . GO GO:0006096; P:glycolysis; IDA:RGD; . GO GO:0001889; P:liver development; IDA:RGD; . GO GO:0031100; P:organ regeneration; IDA:RGD; . GO GO:0042866; P:pyruvate biosynthetic process; IDA:RGD; . GO GO:0009629; P:response to gravity; IEP:RGD; . GO GO:0001666; P:response to hypoxia; IEP:RGD; . GO GO:0032868; P:response to insulin stimulus; IEP:RGD; . GO GO:0014870; P:response to muscle inactivity; IEP:RGD; . GO GO:0007584; P:response to nutrient; IEP:RGD; . GO GO:0043403; P:skeletal muscle tissue regeneration; IDA:RGD; . InterPro IPR001697; Pyr_Knase; . InterPro IPR015813; Pyrv/PenolPyrv_Kinase; . InterPro IPR011037; Pyrv_Knase-like_insert_dom; . InterPro IPR015794; Pyrv_Knase_a/b; . InterPro IPR018209; Pyrv_Knase_AS; . InterPro IPR015793; Pyrv_Knase_brl; . InterPro IPR015795; Pyrv_Knase_C; . InterPro IPR015806; Pyrv_Knase_insert_dom; . Gene3D G3DSA:2.40.33.10; PK_B_barrel_like; 1; . Gene3D G3DSA:3.20.20.60; Pyrv/PenolPyrv_Kinase_cat; 2; . Gene3D G3DSA:3.40.1380.20; Pyrv_Knase_a/b; 1; . KO K00873; -; . PANTHER PTHR11817; Pyruvate_kinase; 1; . Pfam PF00224; PK; 1; . Pfam PF02887; PK_C; 1; . PRINTS PR01050; PYRUVTKNASE; . SUPFAM SSF50800; PK_B_barrel_like; 1; . SUPFAM SSF52935; Pyruvate_kinase; 1; . SUPFAM SSF51621; Pyrv/PenolPyrv_Kinase_cat; 1; . TIGRFAMs TIGR01064; Pyruv_kin; 1; . PROSITE PS00110; PYRUVATE_KINASE; 1; . World-2DPAGE Repository (search AC)