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Searching in 'World-2DPAGE Repository [0022]' for entry matching: MLRV_RAT




World-2DPAGE Repository (0022):  MLRV_RAT


MLRV_RAT


General information about the entry
View entry in simple text format
Entry nameMLRV_RAT
Primary accession numberP08733
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Myosin regulatory light chain 2, ventricular/cardiac muscle isoform; Short=MLC-2; Short=MLC-2v;.
Gene nameName=Myl2
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info
 
HCR_LCR_HEART

MAP LOCATIONS:
pI=4.86; Mw=18868  [identification data]
pI=4.86; Mw=18868  [identification data]
pI=4.86; Mw=18868  [identification data]
pI=4.86; Mw=18868  [identification data]
pI=4.86; Mw=18868  [identification data]
pI=4.86; Mw=18868  [identification data]

IDENTIFICATION: SPOT 66: SeqCov=81.9%. Peptides MS=14/20. Mascot PMF Score=142 [1]
SPOT 83: SeqCov=81.9%. Peptides MS=15/20. Mascot PMF Score=149 [1]; SPOT 110: SeqCov=63.3%. Peptides MS=10/20. Mascot PMF Score=93 [1]; SPOT 118: SeqCov=68.7%. Peptides MS=10/20. Mascot PMF Score=105 [1]; SPOT 125: SeqCov=72.9%. Peptides MS=16/20. Mascot PMF Score=139 [1]; SPOT 810: SeqCov=68.7%. Peptides MS=5/20. Mascot PMF Score=101 [1].
PEPTIDE EVIDENCE: SPOT 110: 51-DTFAALGR-58 (40) / 41-DGFIDKNDLR-50 (59) / 9-RLEGGSSNVFSMFEQTQIQEFK-30 Oxidation (M) (88) [1]
SPOT 118: 51-DTFAALGR-58 (39) / 41-DGFIDKNDLR-50 (33) / 72-EAPGPINFTVFLTMFGEK-89 Oxidation (M) (89) [1]; SPOT 810: 51-DTFAALGR-58 (45) / 41-DGFIDKNDLR-50 (43) / 72-EAPGPINFTVFLTMFGEK-89 Oxidation (M) (82) [1].
MAPPING (identification):
SPOT 66: Peptide mass fingerprinting [1];
SPOT 83: Peptide mass fingerprinting [1];
SPOT 110: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 118: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 125: Peptide mass fingerprinting [1];
SPOT 810: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtP08733; MLRV_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameMLRV_RAT
Primary accession numberP08733
Secondary accession number(s) A0JMZ7
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on November 16, 2011 (version 89)
Name and origin of the protein
DescriptionRecName: Full=Myosin regulatory light chain 2, ventricular/cardiac muscle isoform; Short=MLC-2; Short=MLC-2v;
Gene nameName=Myl2
Encoded onName=Myl2
KeywordsCalcium; Complete proteome; Methylation; Motor protein; Muscle protein; Myosin; Reference proteome; Repeat.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM11851; AAA41621.1; -; mRNA
EMBLX07314; CAA30277.1; -; mRNA
EMBLM30304; AAA41624.1; -; Genomic_DNA
EMBLM30298; AAA41624.1; JOINED; Genomic_DNA
EMBLM30299; AAA41624.1; JOINED; Genomic_DNA
EMBLM30300; AAA41624.1; JOINED; Genomic_DNA
EMBLM30301; AAA41624.1; JOINED; Genomic_DNA
EMBLM30302; AAA41624.1; JOINED; Genomic_DNA
EMBLM30303; AAA41624.1; JOINED; Genomic_DNA
EMBLBC126064; AAI26065.1; -; mRNA
IPIIPI00214000; -; .
PIRS00752; MORT2C; .
RefSeqNP_001030329.1; NM_001035252.1; .
UniGeneRn.37176; -; .
ProteinModelPortalP08733; -; .
PhosphoSiteP08733; -; .
Rat-heart-2DPAGEP08733; -; .
GeneID363925; -; .
KEGGrno:363925; -; .
CTD4633; -; .
RGD1564245; Myl2; .
eggNOGmaNOG19138; -; .
HOVERGENHBG012180; -; .
NextBio684499; -; .
GenevestigatorP08733; -; .
GOGO:0043292; C:contractile fiber; IDA:RGD; .
GOGO:0016459; C:myosin complex; IEA:UniProtKB-KW; .
GOGO:0005509; F:calcium ion binding; IEA:InterPro; .
GOGO:0060048; P:cardiac muscle contraction; IMP:RGD; .
InterProIPR018248; EF-hand; .
InterProIPR011992; EF-hand-like_dom; .
InterProIPR018247; EF_Hand_1_Ca_BS; .
InterProIPR018249; EF_HAND_2; .
InterProIPR002048; EF_hand_Ca-bd; .
Gene3DG3DSA:1.10.238.10; EF-Hand_type; 2; .
KOK10351; -; .
PfamPF00036; efhand; 1; .
SMARTSM00054; EFh; 3; .
PROSITEPS00018; EF_HAND_1; 1; .
PROSITEPS50222; EF_HAND_2; 3; .



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