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Searching in 'World-2DPAGE Repository [0022]' for entry matching: P04636




World-2DPAGE Repository (0022):  P04636


P04636


General information about the entry
View entry in simple text format
Entry nameMDHM_RAT
Primary accession numberP04636
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Malate dehydrogenase, mitochondrial; EC=1.1.1.37; Flags: Precursor;.
Gene nameName=Mdh2
Synonyms=Mor1
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info
 
HCR_LCR_HEART

MAP LOCATIONS:
pI=8.93; Mw=36117  [identification data]
pI=8.93; Mw=36117  [identification data]
pI=8.93; Mw=36117  [identification data]
pI=8.93; Mw=36117  [identification data]
pI=8.93; Mw=36117  [identification data]

IDENTIFICATION: SPOT 119: SeqCov=53.6%. Peptides MS=15/20. Mascot PMF Score=172 [1]
SPOT 139: SeqCov=39.3%. Peptides MS=10/20. Mascot PMF Score=90 [1]; SPOT 302: SeqCov=39.3%. Peptides MS=10/20. Mascot PMF Score=101 [1]; SPOT 312: SeqCov=28.4%. Peptides MS=7/20. Mascot PMF Score=60 [1]; SPOT 527: SeqCov=32.8%. Peptides MS=8/20. Mascot PMF Score=73 [1].
PEPTIDE EVIDENCE: SPOT 139: 79-GYLGPEQLPDCLK-91 (62) / 216-VDFPQDQLATLTGR-229 (72) / 53-LTLYDIAHTPGVAADLSHIETR-74 (96) [1]
SPOT 302: 166-IFGVTTLDIVR-176 (85) / 216-VDFPQDQLATLTGR-229 (96) / 53-LTLYDIAHTPGVAADLSHIETR-74 (124) [1]; SPOT 312: 166-IFGVTTLDIVR-176 (61) / 216-VDFPQDQLATLTGR-229 (82) / 53-LTLYDIAHTPGVAADLSHIETR-74 (87) [1]; SPOT 527: 166-IFGVTTLDIVR-176 (69) / 216-VDFPQDQLATLTGR-229 (88) / 53-LTLYDIAHTPGVAADLSHIETR-74 (113) [1].
MAPPING (identification):
SPOT 119: Peptide mass fingerprinting [1];
SPOT 139: Peptide mass fingerprinting [1];
SPOT 302: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 312: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 527: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtP04636; MDHM_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameMDHM_RAT
Primary accession numberP04636
Secondary accession number(s) Q6GSM4
Sequence was last modified on January 9, 2007 (version 2)
Annotations were last modified on November 16, 2011 (version 115)
Name and origin of the protein
DescriptionRecName: Full=Malate dehydrogenase, mitochondrial; EC=1.1.1.37; Flags: Precursor;
Gene nameName=Mdh2
Synonyms=Mor1
Encoded onName=Mdh2; Synonyms=Mor1
KeywordsAcetylation; Complete proteome; Direct protein sequencing; Mitochondrion; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Transit peptide; Tricarboxylic acid cycle.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX04240; CAA27812.1; -; mRNA
EMBLBC063165; AAH63165.1; -; mRNA
IPIIPI00197696; -; .
PIRA25509; DERTMM; .
RefSeqNP_112413.2; NM_031151.2; .
UniGeneRn.1011; -; .
ProteinModelPortalP04636; -; .
SMRP04636; 25-337; .
IntActP04636; 2; .
STRINGP04636; -; .
PhosphoSiteP04636; -; .
Rat-heart-2DPAGEP04636; -; .
World-2DPAGE0004:P04636; -; .
PRIDEP04636; -; .
EnsemblENSRNOT00000001958; ENSRNOP00000001958; ENSRNOG00000001440; .
GeneID81829; -; .
KEGGrno:81829; -; .
NMPDRfig|10116.3.peg.8382; -; .
UCSCNM_031151; rat; .
CTD4191; -; .
RGD619719; Mdh2; .
eggNOGroNOG11242; -; .
HOVERGENHBG001662; -; .
InParanoidP04636; -; .
OMALVEKCAA; -; .
OrthoDBEOG4MKNGM; -; .
PhylomeDBP04636; -; .
NextBio615775; -; .
ArrayExpressP04636; -; .
GenevestigatorP04636; -; .
GermOnlineENSRNOG00000001440; Rattus norvegicus; .
GOGO:0005759; C:mitochondrial matrix; IDA:RGD; .
GOGO:0030060; F:L-malate dehydrogenase activity; IDA:RGD; .
GOGO:0046554; F:malate dehydrogenase (NADP+) activity; IDA:RGD; .
GOGO:0043621; F:protein self-association; IDA:RGD; .
GOGO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro; .
GOGO:0006475; P:internal protein amino acid acetylation; ISS:UniProtKB; .
GOGO:0006108; P:malate metabolic process; IDA:RGD; .
GOGO:0006734; P:NADH metabolic process; IDA:RGD; .
GOGO:0006107; P:oxaloacetate metabolic process; IDA:RGD; .
GOGO:0006099; P:tricarboxylic acid cycle; IDA:RGD; .
InterProIPR001557; L-lactate/malate_DH; .
InterProIPR022383; Lactate/malate_DH_C; .
InterProIPR001236; Lactate/malate_DH_N; .
InterProIPR015955; Lactate_DH/Glyco_Ohase_4_C; .
InterProIPR001252; Malate_DH_AS; .
InterProIPR010097; Malate_DH_type1; .
InterProIPR016040; NAD(P)-bd_dom; .
Gene3DG3DSA:3.90.110.10; lact_mal_DH; 1; .
Gene3DG3DSA:3.40.50.720; NAD(P)-bd; 1; .
KOK00026; -; .
PANTHERPTHR11540:SF1; MDH_euk_g_bac; 1; .
PfamPF02866; Ldh_1_C; 1; .
PfamPF00056; Ldh_1_N; 1; .
PIRSFPIRSF000102; Lac_mal_DH; 1; .
SUPFAMSSF56327; Lactate_DH/Glyco_hydro_4_C; 1; .
TIGRFAMsTIGR01772; MDH_euk_gproteo; 1; .
PROSITEPS00068; MDH; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server