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Searching in 'World-2DPAGE Repository [0022]' for entry matching: P04797




World-2DPAGE Repository (0022):  P04797

P04797


General information about the entry
View entry in simple text format
Entry nameG3P_RAT
Primary accession numberP04797
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Glyceraldehyde-3-phosphate dehydrogenase; Short=GAPDH; EC=1.2.1.12; AltName: Full=38 kDa BFA-dependent ADP-ribosylation substrate; AltName: Full=BARS-38; AltName: Full=Peptidyl-cysteine S-nitrosylase GAPDH; EC=2.6.99.-;.
Gene nameName=Gapdh
Synonyms=Gapd
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info 		 
HCR_LCR_HEART

MAP LOCATIONS:
pI=8.14; Mw=36090  [identification data]
pI=8.14; Mw=36090  [identification data]
pI=8.14; Mw=36090  [identification data]
pI=8.14; Mw=36090  [identification data]
pI=8.14; Mw=36090  [identification data]

IDENTIFICATION: SPOT 154: SeqCov=23.4%. Peptides MS=7/20. Mascot PMF Score=49 [1]
SPOT 156: SeqCov=33.9%. Peptides MS=10/20. Mascot PMF Score=88 [1]; SPOT 158: SeqCov=27%. Peptides MS=9/20. Mascot PMF Score=74 [1]; SPOT 369: SeqCov=25.5%. Peptides MS=7/20. Mascot PMF Score=50 [1]; SPOT 648: SeqCov=18.6%. Peptides MS=6/20. Mascot PMF Score=37 [1].
PEPTIDE EVIDENCE: SPOT 154: 226-LTGMAFR-232 (28) / 233-VPTPNVSVVDLTCR-246 (52) / 308-LISWYDNEYGYSNR-321 (110) [1]
SPOT 156: 226-LTGMAFR-232 (33) / 233-VPTPNVSVVDLTCR-246 (94) / 308-LISWYDNEYGYSNR-321 (108) [1]; SPOT 158: 226-LTGMAFR-232 (25) / 233-VPTPNVSVVDLTCR-246 (71) / 308-LISWYDNEYGYSNR-321 (113) [1]; SPOT 369: 233-VPTPNVSVVDLTCR-246 (79) / 308-LISWYDNEYGYSNR-321 (127) [1]; SPOT 648: 308-LISWYDNEYGYSNR-321 (108) [1].
MAPPING (identification):
SPOT 154: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 156: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 158: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 369: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 648: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtP04797; G3P_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameG3P_RAT
Primary accession numberP04797
Secondary accession number(s) P09328 Q5M916 Q9QWU4
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on November 16, 2011 (version 125)
Name and origin of the protein
DescriptionRecName: Full=Glyceraldehyde-3-phosphate dehydrogenase; Short=GAPDH; EC=1.2.1.12; AltName: Full=38 kDa BFA-dependent ADP-ribosylation substrate; AltName: Full=BARS-38; AltName: Full=Peptidyl-cysteine S-nitrosylase GAPDH; EC=2.6.99.-;
Gene nameName=Gapdh
Synonyms=Gapd
Encoded onName=Gapdh; Synonyms=Gapd
KeywordsAcetylation; ADP-ribosylation; Apoptosis; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Methylation; NAD; Nucleus; Oxidoreductase; Phosphoprotein; Reference proteome; S-nitrosylation; Transferase; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX02231; CAA26150.1; -; mRNA
EMBLM17701; AAA41193.1; -; mRNA
EMBLAB017801; BAB11748.1; -; mRNA
EMBLAF106860; AAD08929.2; -; mRNA
EMBLBC059110; AAH59110.1; -; mRNA
EMBLBC087743; AAH87743.1; -; mRNA
EMBLM29341; AAA40814.1; -; mRNA
EMBLM11561; AAA41795.1; -; mRNA
IPIIPI00555252; -; .
PIRA23013; DERTG; .
RefSeqNP_058704.1; NM_017008.3; .
RefSeqXP_001062726.1; XM_001062726.2; .
RefSeqXP_002726600.1; XM_002726554.1; .
UniGeneRn.129558; -; .
UniGeneRn.91450; -; .
ProteinModelPortalP04797; -; .
SMRP04797; 2-333; .
IntActP04797; 3; .
MINTMINT-1775142; -; .
STRINGP04797; -; .
PhosphoSiteP04797; -; .
Rat-heart-2DPAGEP04797; -; .
World-2DPAGE0004:P04797; -; .
PRIDEP04797; -; .
EnsemblENSRNOT00000050443; ENSRNOP00000040878; ENSRNOG00000018630; .
GeneID24383; -; .
GeneID685186; -; .
KEGGrno:24383; -; .
KEGGrno:685186; -; .
UCSCNM_017008; rat; .
CTD2597; -; .
RGD2661; Gapdh; .
eggNOGmaNOG06292; -; .
HOVERGENHBG000227; -; .
InParanoidP04797; -; .
OrthoDBEOG4Q84XS; -; .
PhylomeDBP04797; -; .
NextBio603149; -; .
GenevestigatorP04797; -; .
GermOnlineENSRNOG00000033057; Rattus norvegicus; .
GOGO:0005829; C:cytosol; IDA:UniProtKB; .
GOGO:0005634; C:nucleus; IDA:UniProtKB; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; IDA:UniProtKB; .
GOGO:0051287; F:NAD binding; IEA:InterPro; .
GOGO:0035605; F:peptidyl-cysteine S-nitrosylase activity; IDA:UniProtKB; .
GOGO:0005515; F:protein binding; IPI:UniProtKB; .
GOGO:0006094; P:gluconeogenesis; IDA:RGD; .
GOGO:0006096; P:glycolysis; IDA:RGD; .
GOGO:0051402; P:neuron apoptosis; IDA:UniProtKB; .
GOGO:0035606; P:peptidyl-cysteine S-trans-nitrosylation; IDA:UniProtKB; .
GOGO:0050821; P:protein stabilization; IDA:UniProtKB; .
InterProIPR020831; GlycerAld/Erythrose_P_DH; .
InterProIPR020830; GlycerAld_3-P_DH_AS; .
InterProIPR020829; GlycerAld_3-P_DH_cat; .
InterProIPR020828; GlycerAld_3-P_DH_NAD(P)-bd; .
InterProIPR006424; Glyceraldehyde-3-P_DH_1; .
InterProIPR016040; NAD(P)-bd_dom; .
Gene3DG3DSA:3.40.50.720; NAD(P)-bd; 1; .
KOK00134; -; .
PANTHERPTHR10836; GAP_DH; 1; .
PfamPF02800; Gp_dh_C; 1; .
PfamPF00044; Gp_dh_N; 1; .
PIRSFPIRSF000149; GAP_DH; 1; .
PRINTSPR00078; G3PDHDRGNASE; .
SMARTSM00846; Gp_dh_N; 1; .
TIGRFAMsTIGR01534; GAPDH-I; 1; .
PROSITEPS00071; GAPDH; 1; .


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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server