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Searching in 'World-2DPAGE Repository [0022]' for entry matching: P04797 World-2DPAGE Repository (0022):  P04797 P04797 General information about the entry View entry in simple text format Entry name G3P_RAT Primary accession number P04797 integrated into World-2DPAGE Repository (0022) on  May 7, 2010 (release 1) 2D Annotations were last modified on June 22, 2011 (version 2) General Annotations were last modified on  November 24, 2011 (version 2) Name and origin of the protein Description RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase; Short=GAPDH; EC=1.2.1.12; AltName: Full=38 kDa BFA-dependent ADP-ribosylation substrate; AltName: Full=BARS-38; AltName: Full=Peptidyl-cysteine S-nitrosylase GAPDH; EC=2.6.99.-;. Gene name Name=Gapdh Synonyms=Gapd Annotated species Rattus [TaxID: 10114] Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae. References [1]   2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000593; Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L. ''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity'' Proteomics 11(16):3369-3379 (2011) 2D PAGE maps for identified proteins How to interpret a protein HCR_LCR_HEART {HCR LCR Cardiac Map} Rattus Tissue: Heart   map experimental info   HCR_LCR_HEART MAP LOCATIONS: SPOT 154: pI=8.14; Mw=36090   [identification data] SPOT 156: pI=8.14; Mw=36090   [identification data] SPOT 158: pI=8.14; Mw=36090   [identification data] SPOT 369: pI=8.14; Mw=36090   [identification data] SPOT 648: pI=8.14; Mw=36090   [identification data] IDENTIFICATION: SPOT 154: SeqCov=23.4%. Peptides MS=7/20. Mascot PMF Score=49 [1] SPOT 156: SeqCov=33.9%. Peptides MS=10/20. Mascot PMF Score=88 [1]; SPOT 158: SeqCov=27%. Peptides MS=9/20. Mascot PMF Score=74 [1]; SPOT 369: SeqCov=25.5%. Peptides MS=7/20. Mascot PMF Score=50 [1]; SPOT 648: SeqCov=18.6%. Peptides MS=6/20. Mascot PMF Score=37 [1]. PEPTIDE EVIDENCE: SPOT 154: 226-LTGMAFR-232 (28) / 233-VPTPNVSVVDLTCR-246 (52) / 308-LISWYDNEYGYSNR-321 (110) [1] SPOT 156: 226-LTGMAFR-232 (33) / 233-VPTPNVSVVDLTCR-246 (94) / 308-LISWYDNEYGYSNR-321 (108) [1]; SPOT 158: 226-LTGMAFR-232 (25) / 233-VPTPNVSVVDLTCR-246 (71) / 308-LISWYDNEYGYSNR-321 (113) [1]; SPOT 369: 233-VPTPNVSVVDLTCR-246 (79) / 308-LISWYDNEYGYSNR-321 (127) [1]; SPOT 648: 308-LISWYDNEYGYSNR-321 (108) [1]. MAPPING (identification): SPOT 154: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1]; SPOT 156: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1]; SPOT 158: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1]; SPOT 369: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1]; SPOT 648: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1]. Copyright Data from Dr. Jatin Burniston, Liverpool John Moores University, UK Cross-references UniProtKB/Swiss-Prot P04797; G3P_RAT. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_11 Entry name G3P_RAT Primary accession number P04797 Secondary accession number(s) P09328 Q5M916 Q9QWU4 Sequence was last modified on  January 23, 2007 (version 3) Annotations were last modified on  November 16, 2011 (version 125) Name and origin of the protein Description RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase; Short=GAPDH; EC=1.2.1.12; AltName: Full=38 kDa BFA-dependent ADP-ribosylation substrate; AltName: Full=BARS-38; AltName: Full=Peptidyl-cysteine S-nitrosylase GAPDH; EC=2.6.99.-; Gene name Name=Gapdh Synonyms=Gapd Encoded on Name=Gapdh; Synonyms=Gapd Keywords Acetylation; ADP-ribosylation; Apoptosis; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Methylation; NAD; Nucleus; Oxidoreductase; Phosphoprotein; Reference proteome; S-nitrosylation; Transferase; Ubl conjugation. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL X02231; CAA26150.1; -; mRNA EMBL M17701; AAA41193.1; -; mRNA EMBL AB017801; BAB11748.1; -; mRNA EMBL AF106860; AAD08929.2; -; mRNA EMBL BC059110; AAH59110.1; -; mRNA EMBL BC087743; AAH87743.1; -; mRNA EMBL M29341; AAA40814.1; -; mRNA EMBL M11561; AAA41795.1; -; mRNA IPI IPI00555252; -; . PIR A23013; DERTG; . RefSeq NP_058704.1; NM_017008.3; . RefSeq XP_001062726.1; XM_001062726.2; . RefSeq XP_002726600.1; XM_002726554.1; . UniGene Rn.129558; -; . UniGene Rn.91450; -; . ProteinModelPortal P04797; -; . SMR P04797; 2-333; . IntAct P04797; 3; . MINT MINT-1775142; -; . STRING P04797; -; . PhosphoSite P04797; -; . Rat-heart-2DPAGE P04797; -; . World-2DPAGE 0004:P04797; -; . PRIDE P04797; -; . Ensembl ENSRNOT00000050443; ENSRNOP00000040878; ENSRNOG00000018630; . GeneID 24383; -; . GeneID 685186; -; . KEGG rno:24383; -; . KEGG rno:685186; -; . UCSC NM_017008; rat; . CTD 2597; -; . RGD 2661; Gapdh; . eggNOG maNOG06292; -; . HOVERGEN HBG000227; -; . InParanoid P04797; -; . OrthoDB EOG4Q84XS; -; . PhylomeDB P04797; -; . NextBio 603149; -; . Genevestigator P04797; -; . GermOnline ENSRNOG00000033057; Rattus norvegicus; . GO GO:0005829; C:cytosol; IDA:UniProtKB; . GO GO:0005634; C:nucleus; IDA:UniProtKB; . GO GO:0005625; C:soluble fraction; IDA:RGD; . GO GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; IDA:UniProtKB; . GO GO:0051287; F:NAD binding; IEA:InterPro; . GO GO:0035605; F:peptidyl-cysteine S-nitrosylase activity; IDA:UniProtKB; . GO GO:0005515; F:protein binding; IPI:UniProtKB; . GO GO:0006094; P:gluconeogenesis; IDA:RGD; . GO GO:0006096; P:glycolysis; IDA:RGD; . GO GO:0051402; P:neuron apoptosis; IDA:UniProtKB; . GO GO:0035606; P:peptidyl-cysteine S-trans-nitrosylation; IDA:UniProtKB; . GO GO:0050821; P:protein stabilization; IDA:UniProtKB; . InterPro IPR020831; GlycerAld/Erythrose_P_DH; . InterPro IPR020830; GlycerAld_3-P_DH_AS; . InterPro IPR020829; GlycerAld_3-P_DH_cat; . InterPro IPR020828; GlycerAld_3-P_DH_NAD(P)-bd; . InterPro IPR006424; Glyceraldehyde-3-P_DH_1; . InterPro IPR016040; NAD(P)-bd_dom; . Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1; . KO K00134; -; . PANTHER PTHR10836; GAP_DH; 1; . Pfam PF02800; Gp_dh_C; 1; . Pfam PF00044; Gp_dh_N; 1; . PIRSF PIRSF000149; GAP_DH; 1; . PRINTS PR00078; G3PDHDRGNASE; . SMART SM00846; Gp_dh_N; 1; . TIGRFAMs TIGR01534; GAPDH-I; 1; . PROSITE PS00071; GAPDH; 1; . World-2DPAGE Repository (search AC)