World-2DPAGE Repository

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1111/j.1471-4159.2010.06719.x; VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M. ''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome'' J of Neurochemistry 113(6):1577-1588 (2010)

2D PAGE maps for identified proteins

How to interpret a protein

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus

map experimental info

HIPP_SYNAP

MAP LOCATIONS:

SPOT 531:

pI=5.32; Mw=40883

[identification data]

SPOT 535:

pI=5.40; Mw=45561

[identification data]

SPOT 1117:

pI=5.32; Mw=40883

[identification data]

SPOT 1174:

pI=5.33; Mw=42984

[identification data]

IDENTIFICATION: SPOT 531: Peptides number=5. SeqCov=21.3%. MOWSE score=2.40E+6 [1]
SPOT 535: Peptides number=13. SeqCov=55.6%. MOWSE score=1.02E+16 [1]; SPOT 1117: Peptides number=6. SeqCov=23.9%. MOWSE score=7.85E+6 [1]; SPOT 1174: Peptides number=13. SeqCov=45.9%. MOWSE score=5.86E+10 [1].
MAPPING (identification):

SPOT 531: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 535: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 1117: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 1174: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Willard Freeman, Penn State College of Medicine, USA

Cross-references

UniProtKB/Swiss-Prot

P07335; KCRB_RAT.

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	KCRB_RAT
Primary accession number	P07335
Secondary accession number(s)	A0JPK7 Q499P7 Q5PPJ5 Q6IRE0 Q6P139
Sequence was last modified on	January 9, 2007 (version 2)
Annotations were last modified on	November 16, 2011 (version 102)
Name and origin of the protein
Description	RecName: Full=Creatine kinase B-type; EC=2.7.3.2; AltName: Full=B-CK; AltName: Full=Creatine kinase B chain;
Gene name	Name=Ckb Synonyms=Ckbb
Encoded on	Name=Ckb; Synonyms=Ckbb
Keywords	ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	M14400; AAA40930.1; -; mRNA
EMBL	M18668; AAA40932.1; -; Genomic_DNA
EMBL	M57664; AAA40933.1; -; mRNA
EMBL	M57665; AAA40931.1; -; Genomic_DNA
EMBL	BC065307; AAH65307.2; -; mRNA
EMBL	BC070955; AAH70955.2; -; mRNA
EMBL	BC087656; AAH87656.1; ALT_INIT; mRNA
EMBL	BC099814; AAH99814.2; -; mRNA
EMBL	BC127477; AAI27478.2; -; mRNA
IPI	IPI00470288; -; .
PIR	A23980; KIRTCB; .
RefSeq	NP_036661.2; NM_012529.2; .
UniGene	Rn.1472; -; .
ProteinModelPortal	P07335; -; .
SMR	P07335; 2-381; .
STRING	P07335; -; .
PhosphoSite	P07335; -; .
World-2DPAGE	0004:P07335; -; .
PRIDE	P07335; -; .
Ensembl	ENSRNOT00000015122; ENSRNOP00000015122; ENSRNOG00000010872; .
GeneID	24264; -; .
KEGG	rno:24264; -; .
UCSC	NM_012529; rat; .
CTD	1152; -; .
RGD	2357; Ckb; .
eggNOG	roNOG15359; -; .
GeneTree	ENSGT00550000074561; -; .
HOVERGEN	HBG001339; -; .
InParanoid	P07335; -; .
OrthoDB	EOG4R7V9X; -; .
NextBio	602815; -; .
ArrayExpress	P07335; -; .
Genevestigator	P07335; -; .
GermOnline	ENSRNOG00000010872; Rattus norvegicus; .
GO	GO:0005886; C:plasma membrane; TAS:RGD; .
GO	GO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GO	GO:0004111; F:creatine kinase activity; TAS:RGD; .
GO	GO:0005515; F:protein binding; IPI:RGD; .
GO	GO:0007420; P:brain development; IEP:RGD; .
GO	GO:0030644; P:cellular chloride ion homeostasis; IMP:RGD; .
GO	GO:0006603; P:phosphocreatine metabolic process; TAS:RGD; .
InterPro	IPR000749; ATP-guanido_PTrfase; .
InterPro	IPR022415; ATP-guanido_PTrfase_AS; .
InterPro	IPR022414; ATP-guanido_PTrfase_cat; .
InterPro	IPR022413; ATP-guanido_PTrfase_N; .
InterPro	IPR014746; Gln_synth/guanido_kin_cat_dom; .
Gene3D	G3DSA:1.10.135.10; ATP-gua_Ptrans; 1; .
Gene3D	G3DSA:3.30.590.10; ATP-gua_Ptrans; 1; .
KO	K00933; -; .
PANTHER	PTHR11547; ATP-gua_Ptrans; 1; .
Pfam	PF00217; ATP-gua_Ptrans; 1; .
Pfam	PF02807; ATP-gua_PtransN; 1; .
SUPFAM	SSF48034; ATP-gua_Ptrans; 1; .
PROSITE	PS00112; PHOSPHAGEN_KINASE; 1; .
PROSITE	PS51510; PHOSPHAGEN_KINASE_C; 1; .
PROSITE	PS51509; PHOSPHAGEN_KINASE_N; 1; .

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server

0001
0002
0003
0004
0005
0006
0007
0008
0009
0010
0011
0012
0014
0017
0018
0019
0020
0021
0022
0023
0024
0026
0027
0029
0030
0031
0032
0034
0035
0036
0037
0038
0039
0040
0042
0043
0044
0046
0047
0050
0051
0053
0054
0056
0059
0061
0062
0063
0066
0068
0069
0076
0077
0080
0084
0085
0088