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Searching in 'World-2DPAGE Repository [0021]' for entry matching: P08226




World-2DPAGE Repository (0021):  P08226


P08226


General information about the entry
View entry in simple text format
Entry nameAPOE_MOUSE
Primary accession numberP08226
integrated into World-2DPAGE Repository (0021) on June 9, 2010 (release 1)
2D Annotations were last modified onJune 20, 2011 (version 2)
General Annotations were last modified on November 30, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Apolipoprotein E; Short=Apo-E; Flags: Precursor;.
Gene nameName=Apoe
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
De Cegli R., Romito A., Iacobacci S., Mao L., Lauria M., Fedele A.O., Klose J., Borel C., Descombes P., Antonarakis S.E., Di Bernardo D., Banfi S., Ballabio A., Cobellis G.
''A mouse Embryonic Stem Cell Bank for inducible overexpression of human Chromosome 21 genes''
Genome Biol 11(6):R64 (2010)
2D PAGE maps for identified proteins
How to interpret a protein

RUNX1_DAY7 {Mouse embryonic stem cells overexpressing Runx1, Day 7}
Mus musculus (Mouse)
Tissue: Embryonic stem cell
RUNX1_DAY7
  map experimental info
 
RUNX1_DAY7

MAP LOCATIONS:
pI=5.82; Mw=33200  [identification data]

EXPRESSION:
SPOT 3078: Protein ratio 48h/0h=3.28. mRNA ratio 48h/0h=0.8 [1].

IDENTIFICATION: SPOT 3078: Mascot Protein score=301. SeqCov=28%. Peptides identified=16 [1].
MAPPING (identification):
SPOT 3078: Tandem mass spectrometry [1].

Copyright
Data from Dr. Lei Mao, Institute of Human Genetics, Charite Universitaetsmedizin Berlin, Germany
Cross-references
UniProtKB/Swiss-ProtP08226; APOE_MOUSE.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameAPOE_MOUSE
Primary accession numberP08226
Sequence was last modified on August 1, 1990 (version 2)
Annotations were last modified on November 16, 2011 (version 110)
Name and origin of the protein
DescriptionRecName: Full=Apolipoprotein E; Short=Apo-E; Flags: Precursor;
Gene nameName=Apoe
Encoded onName=Apoe
Keywords3D-structure; Chylomicron; Complete proteome; Direct protein sequencing; HDL; Heparin-binding; Lipid transport; Phosphoprotein; Reference proteome; Repeat; Secreted; Signal; Transport; VLDL.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLD00466; BAA00361.1; -; Genomic_DNA
EMBLM12414; AAA37251.1; -; mRNA
EMBLBC083351; AAH83351.1; -; mRNA
EMBLM73490; AAA37252.1; -; mRNA
IPIIPI00323571; -; .
PIRJU0036; JU0036; .
RefSeqNP_033826.2; NM_009696.3; .
UniGeneMm.305152; -; .
PDB1YA9; X-ray; 2.09 A; A=20-200
PDBsum1YA9; -; .
ProteinModelPortalP08226; -; .
SMRP08226; 19-305; .
IntActP08226; 4; .
MINTMINT-242645; -; .
STRINGP08226; -; .
PhosphoSiteP08226; -; .
PRIDEP08226; -; .
EnsemblENSMUST00000003066; ENSMUSP00000003066; ENSMUSG00000002985; .
EnsemblENSMUST00000173739; ENSMUSP00000133371; ENSMUSG00000002985; .
EnsemblENSMUST00000174064; ENSMUSP00000133302; ENSMUSG00000002985; .
EnsemblENSMUST00000174355; ENSMUSP00000134160; ENSMUSG00000002985; .
GeneID11816; -; .
KEGGmmu:11816; -; .
CTD348; -; .
MGIMGI:88057; Apoe; .
eggNOGroNOG10307; -; .
HOGENOMHBG126848; -; .
HOVERGENHBG010582; -; .
InParanoidP08226; -; .
OMAEMGSRTR; -; .
OrthoDBEOG4GB76S; -; .
PhylomeDBP08226; -; .
NextBio279703; -; .
ArrayExpressP08226; -; .
BgeeP08226; -; .
CleanExMM_APOE; -; .
GenevestigatorP08226; -; .
GermOnlineENSMUSG00000002985; Mus musculus; .
GOGO:0042627; C:chylomicron; IEA:UniProtKB-KW; .
GOGO:0034364; C:high-density lipoprotein particle; IEA:UniProtKB-KW; .
GOGO:0034362; C:low-density lipoprotein particle; IDA:BHF-UCL; .
GOGO:0034361; C:very-low-density lipoprotein particle; IEA:UniProtKB-KW; .
GOGO:0001540; F:beta-amyloid binding; ISS:UniProtKB; .
GOGO:0017127; F:cholesterol transporter activity; IDA:MGI; .
GOGO:0008201; F:heparin binding; IEA:UniProtKB-KW; .
GOGO:0008289; F:lipid binding; IEA:InterPro; .
GOGO:0071813; F:lipoprotein particle binding; IDA:MGI; .
GOGO:0060228; F:phosphatidylcholine-sterol O-acyltransferase activator activity; IMP:BHF-UCL; .
GOGO:0048844; P:artery morphogenesis; IGI:MGI; .
GOGO:0006874; P:cellular calcium ion homeostasis; IDA:MGI; .
GOGO:0006707; P:cholesterol catabolic process; IMP:MGI; .
GOGO:0033344; P:cholesterol efflux; IDA:MGI; .
GOGO:0042632; P:cholesterol homeostasis; IMP:MGI; .
GOGO:0006917; P:induction of apoptosis; ISS:UniProtKB; .
GOGO:0042158; P:lipoprotein biosynthetic process; IGI:MGI; .
GOGO:0042159; P:lipoprotein catabolic process; IMP:MGI; .
GOGO:0034374; P:low-density lipoprotein particle remodeling; IMP:BHF-UCL; .
GOGO:0051651; P:maintenance of location in cell; IMP:MGI; .
GOGO:0010873; P:positive regulation of cholesterol esterification; IMP:BHF-UCL; .
GOGO:0010468; P:regulation of gene expression; IMP:MGI; .
GOGO:0002021; P:response to dietary excess; IMP:MGI; .
GOGO:0006979; P:response to oxidative stress; IMP:MGI; .
GOGO:0042311; P:vasodilation; IMP:MGI; .
GOGO:0034372; P:very-low-density lipoprotein particle remodeling; IMP:BHF-UCL; .
InterProIPR013326; ApoA/E_ApoLp; .
InterProIPR000074; ApoA1_A4_E; .
Gene3DG3DSA:1.20.120.20; ApoA/E_ApoLp; 2; .
KOK04524; -; .
PfamPF01442; Apolipoprotein; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server