World-2DPAGE Repository

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.200900680; Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of whole cerebellum of the mature rat'' Proteomics 10(23):4311-4319 (2010)

2D PAGE maps for identified proteins

How to interpret a protein

RAT_CEREBELLUM_21D {Proteome of 21 days old rat cerebellum}
Rattus norvegicus (Rat)
Tissue: Cerebellum

map experimental info

RAT_CEREBELLUM_21D

MAP LOCATIONS:

SPOT 3373:

pI=6.62; Mw=45000

[identification data]

IDENTIFICATION: SPOT 3373: SeqCov=37%. MascotPMF=128 [1].
MAPPING (identification):

SPOT 3373: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

PRIDE

10017.

UniProtKB/Swiss-Prot

P10760; SAHH_RAT.

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	SAHH_RAT
Primary accession number	P10760
Sequence was last modified on	January 23, 2007 (version 3)
Annotations were last modified on	November 16, 2011 (version 120)
Name and origin of the protein
Description	RecName: Full=Adenosylhomocysteinase; Short=AdoHcyase; EC=3.3.1.1; AltName: Full=S-adenosyl-L-homocysteine hydrolase;
Gene name	Name=Ahcy
Encoded on	Name=Ahcy
Keywords	3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; NAD; One-carbon metabolism; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	M15185; AAA40705.1; -; mRNA
EMBL	U14937; AAA92043.1; -; Genomic_DNA
IPI	IPI00476295; -; .
PIR	A26583; A26583; .
RefSeq	NP_058897.1; NM_017201.1; .
UniGene	Rn.5878; -; .
PDB	1B3R; X-ray; 2.80 A; A/B/C/D=2-432
PDB	1D4F; X-ray; 2.80 A; A/B/C/D=2-432
PDB	1K0U; X-ray; 3.00 A; A/B/C/D/E/F/G/H=2-432
PDB	1KY4; X-ray; 2.80 A; A/B/C/D=2-432
PDB	1KY5; X-ray; 2.80 A; A/B/C/D=2-432
PDB	1XWF; X-ray; 2.80 A; A/B/C/D=2-431
PDB	2H5L; X-ray; 2.80 A; A/B/C/D/E/F/G/H=2-431
PDBsum	1B3R; -; .
PDBsum	1D4F; -; .
PDBsum	1K0U; -; .
PDBsum	1KY4; -; .
PDBsum	1KY5; -; .
PDBsum	1XWF; -; .
PDBsum	2H5L; -; .
ProteinModelPortal	P10760; -; .
SMR	P10760; 3-432; .
IntAct	P10760; 1; .
STRING	P10760; -; .
World-2DPAGE	0004:P10760; -; .
PRIDE	P10760; -; .
Ensembl	ENSRNOT00000024310; ENSRNOP00000024310; ENSRNOG00000017777; .
GeneID	29443; -; .
KEGG	rno:29443; -; .
NMPDR	fig\|10116.3.peg.20074; -; .
UCSC	NM_017201; rat; .
CTD	191; -; .
RGD	69260; Ahcy; .
eggNOG	roNOG13500; -; .
HOVERGEN	HBG005041; -; .
InParanoid	P10760; -; .
OMA	ELFTKTE; -; .
OrthoDB	EOG4MW862; -; .
PhylomeDB	P10760; -; .
BioCyc	MetaCyc:MONOMER-8582; -; .
BRENDA	3.3.1.1; 5301; .
NextBio	609197; -; .
ArrayExpress	P10760; -; .
Genevestigator	P10760; -; .
GermOnline	ENSRNOG00000017777; Rattus norvegicus; .
GO	GO:0005829; C:cytosol; IDA:RGD; .
GO	GO:0042470; C:melanosome; IEA:UniProtKB-SubCell; .
GO	GO:0005634; C:nucleus; IDA:RGD; .
GO	GO:0019717; C:synaptosome; IDA:RGD; .
GO	GO:0004013; F:adenosylhomocysteinase activity; IDA:RGD; .
GO	GO:0030554; F:adenyl nucleotide binding; IDA:RGD; .
GO	GO:0042802; F:identical protein binding; IDA:RGD; .
GO	GO:0051287; F:NAD binding; IDA:RGD; .
GO	GO:0002439; P:chronic inflammatory response to antigenic stimulus; IMP:RGD; .
GO	GO:0042745; P:circadian sleep/wake cycle; IDA:RGD; .
GO	GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW; .
GO	GO:0001666; P:response to hypoxia; IEP:RGD; .
GO	GO:0007584; P:response to nutrient; IDA:RGD; .
GO	GO:0019510; P:S-adenosylhomocysteine catabolic process; IDA:RGD; .
InterPro	IPR000043; Adenosylhomocysteinase; .
InterPro	IPR015878; Ado_hCys_hydrolase_NAD-bd; .
InterPro	IPR020082; S-Ado-L-homoCys_hydrolase_CS; .
KO	K01251; -; .
PANTHER	PTHR23420; Ad_hcy_hydrolase; 1; .
Pfam	PF05221; AdoHcyase; 1; .
Pfam	PF00670; AdoHcyase_NAD; 1; .
PIRSF	PIRSF001109; Ad_hcy_hydrolase; 1; .
TIGRFAMs	TIGR00936; AhcY; 1; .
PROSITE	PS00738; ADOHCYASE_1; 1; .
PROSITE	PS00739; ADOHCYASE_2; 1; .

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server

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