World-2DPAGE Repository

RecName: Full=Protein disulfide-isomerase A3; EC=5.3.4.1; AltName: Full=58 kDa glucose-regulated protein; AltName: Full=58 kDa microsomal protein; Short=p58; AltName: Full=Disulfide isomerase ER-60; AltName: Full=Endoplasmic reticulum resident protein 57; Short=ER protein 57; Short=ERp57; AltName: Full=Endoplasmic reticulum resident protein 60; Short=ER protein 60; Short=ERp60; AltName: Full=HIP-70; AltName: Full=Q-2; Flags: Precursor;.

Gene name

Name=Pdia3
Synonyms=Erp60, Grp58

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.200900680; Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of whole cerebellum of the mature rat'' Proteomics 10(23):4311-4319 (2010)

2D PAGE maps for identified proteins

How to interpret a protein

RAT_CEREBELLUM_21D {Proteome of 21 days old rat cerebellum}
Rattus norvegicus (Rat)
Tissue: Cerebellum

map experimental info

RAT_CEREBELLUM_21D

MAP LOCATIONS:

SPOT 2835:

pI=5.88; Mw=57000

[identification data]

SPOT 2837:

pI=5.79; Mw=58000

[identification data]

IDENTIFICATION: SPOT 2835: SeqCov=48%. MascotPMF=215 [1]
SPOT 2837: SeqCov=50%. MascotPMF=273 [1].
MAPPING (identification):

SPOT 2835: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 2837: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

PRIDE

10017.

UniProtKB/Swiss-Prot

P11598; PDIA3_RAT.

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	PDIA3_RAT
Primary accession number	P11598
Sequence was last modified on	February 1, 1996 (version 2)
Annotations were last modified on	November 16, 2011 (version 124)
Name and origin of the protein
Description	RecName: Full=Protein disulfide-isomerase A3; EC=5.3.4.1; AltName: Full=58 kDa glucose-regulated protein; AltName: Full=58 kDa microsomal protein; Short=p58; AltName: Full=Disulfide isomerase ER-60; AltName: Full=Endoplasmic reticulum resident protein 57; Short=ER protein 57; Short=ERp57; AltName: Full=Endoplasmic reticulum resident protein 60; Short=ER protein 60; Short=ERp60; AltName: Full=HIP-70; AltName: Full=Q-2; Flags: Precursor;
Gene name	Name=Pdia3 Synonyms=Erp60, Grp58
Encoded on	Name=Pdia3; Synonyms=Erp60, Grp58
Keywords	Complete proteome; Direct protein sequencing; Disulfide bond; Endoplasmic reticulum; Isomerase; Redox-active center; Reference proteome; Repeat; Signal.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	X12355; CAA30916.1; -; mRNA
EMBL	D63378; BAA09695.1; -; mRNA
EMBL	BC062393; AAH62393.1; -; mRNA
IPI	IPI00324741; -; .
PIR	A28807; A28807; .
PIR	A61354; A61354; .
RefSeq	NP_059015.1; NM_017319.1; .
UniGene	Rn.11527; -; .
ProteinModelPortal	P11598; -; .
SMR	P11598; 25-488; .
IntAct	P11598; 2; .
MINT	MINT-4575564; -; .
STRING	P11598; -; .
PhosphoSite	P11598; -; .
World-2DPAGE	0004:P11598; -; .
PRIDE	P11598; -; .
Ensembl	ENSRNOT00000020478; ENSRNOP00000020478; ENSRNOG00000015018; .
GeneID	29468; -; .
KEGG	rno:29468; -; .
UCSC	NM_017319; rat; .
CTD	2923; -; .
RGD	68430; Pdia3; .
eggNOG	roNOG09779; -; .
GeneTree	ENSGT00590000082864; -; .
HOVERGEN	HBG005920; -; .
InParanoid	P11598; -; .
OrthoDB	EOG42Z4PX; -; .
PhylomeDB	P11598; -; .
NextBio	609284; -; .
ArrayExpress	P11598; -; .
Genevestigator	P11598; -; .
GermOnline	ENSRNOG00000015018; Rattus norvegicus; .
GO	GO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell; .
GO	GO:0042470; C:melanosome; IEA:UniProtKB-SubCell; .
GO	GO:0009055; F:electron carrier activity; IEA:InterPro; .
GO	GO:0003756; F:protein disulfide isomerase activity; IEA:EC; .
GO	GO:0015035; F:protein disulfide oxidoreductase activity; IEA:InterPro; .
GO	GO:0045454; P:cell redox homeostasis; IEA:InterPro; .
GO	GO:0006662; P:glycerol ether metabolic process; IEA:InterPro; .
InterPro	IPR005788; Disulphide_isomerase; .
InterPro	IPR005792; Prot_disulphide_isomerase; .
InterPro	IPR005746; Thioredoxin; .
InterPro	IPR012336; Thioredoxin-like_fold; .
InterPro	IPR017937; Thioredoxin_CS; .
InterPro	IPR013766; Thioredoxin_domain; .
Gene3D	G3DSA:3.40.30.10; Thioredoxin_fold; 3; .
KO	K08056; -; .
Pfam	PF00085; Thioredoxin; 2; .
PRINTS	PR00421; THIOREDOXIN; .
SUPFAM	SSF52833; Thiordxn-like_fd; 4; .
TIGRFAMs	TIGR01130; ER_PDI_fam; 1; .
TIGRFAMs	TIGR01126; Pdi_dom; 2; .
PROSITE	PS00194; THIOREDOXIN_1; 2; .
PROSITE	PS51352; THIOREDOXIN_2; 2; .

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server

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