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Searching in 'World-2DPAGE Repository [0030]' for entry matching: P12928

World-2DPAGE Repository (0030):  P12928


General information about the entry
View entry in simple text format
Entry nameKPYR_RAT
Primary accession numberP12928
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Pyruvate kinase isozymes R/L; EC=; AltName: Full=L-PK;.
Gene nameName=Pklr
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
  map experimental info

pI=6.55; Mw=67180  [identification data]
pI=6.54; Mw=66257  [identification data]
pI=5.92; Mw=61064  [identification data]

IDENTIFICATION: SPOT 855: Mascot protein score=112. Number of peptides=7 [1]
SPOT 891: Mascot protein score=36. Number of peptides=2 [1]; SPOT 1051: Mascot protein score=62. Number of peptides=4 [1].
MAPPING (identification):
SPOT 855: Tandem mass spectrometry [1];
SPOT 891: Tandem mass spectrometry [1];
SPOT 1051: Tandem mass spectrometry [1].

Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
UniProtKB/Swiss-ProtP12928; KPYR_RAT.
World-2DPAGE RepositoryP12928; KPYR_RAT.

2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameKPYR_RAT
Primary accession numberP12928
Secondary accession number(s) P04763 Q64618
Sequence was last modified on February 1, 1995 (version 2)
Annotations were last modified on November 16, 2011 (version 116)
Name and origin of the protein
DescriptionRecName: Full=Pyruvate kinase isozymes R/L; EC=; AltName: Full=L-PK;
Gene nameName=Pklr
Encoded onName=Pklr
KeywordsAcetylation; Allosteric enzyme; Alternative splicing; ATP-binding; Complete proteome; Glycolysis; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Potassium; Pyruvate; Reference proteome; Transferase.
Copyrighted by the UniProt Consortium, see Distributed under the Creative Commons Attribution-NoDerivs License
EMBLM17091; AAA41882.1; -; Genomic_DNA
EMBLM17088; AAA41882.1; JOINED; Genomic_DNA
EMBLM17089; AAA41882.1; JOINED; Genomic_DNA
EMBLM17090; AAA41882.1; JOINED; Genomic_DNA
EMBLM17091; AAA41883.1; -; Genomic_DNA
EMBLM17088; AAA41883.1; JOINED; Genomic_DNA
EMBLM17089; AAA41883.1; JOINED; Genomic_DNA
EMBLM17090; AAA41883.1; JOINED; Genomic_DNA
EMBLM17685; AAA41881.1; -; mRNA
EMBLX05684; CAA29169.1; -; Genomic_DNA
EMBLM11709; AAA41880.1; -; mRNA
IPIIPI00202549; -; .
IPIIPI00231683; -; .
PIRA27427; KIRTPR; .
PIRA92940; KIRTPL; .
RefSeqNP_036756.3; NM_012624.3; .
UniGeneRn.48821; -; .
ProteinModelPortalP12928; -; .
SMRP12928; 57-573; .
STRINGP12928; -; .
PhosphoSiteP12928; -; .
PRIDEP12928; -; .
EnsemblENSRNOT00000027700; ENSRNOP00000027700; ENSRNOG00000020420; .
EnsemblENSRNOT00000065791; ENSRNOP00000058886; ENSRNOG00000020420; .
GeneID24651; -; .
KEGGrno:24651; -; .
NMPDRfig|10116.3.peg.16404; -; .
UCSCNM_012624; rat; .
CTD5313; -; .
RGD3336; Pklr; .
eggNOGmaNOG16019; -; .
GeneTreeENSGT00390000008859; -; .
HOVERGENHBG000941; -; .
InParanoidP12928; -; .
OrthoDBEOG40GCQJ; -; .
PhylomeDBP12928; -; .
ArrayExpressP12928; -; .
GenevestigatorP12928; -; .
GermOnlineENSRNOG00000020420; Rattus norvegicus; .
GOGO:0005829; C:cytosol; IDA:RGD; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0000287; F:magnesium ion binding; IEA:InterPro; .
GOGO:0030955; F:potassium ion binding; IEA:InterPro; .
GOGO:0004743; F:pyruvate kinase activity; IDA:RGD; .
GOGO:0006754; P:ATP biosynthetic process; IDA:RGD; .
GOGO:0032869; P:cellular response to insulin stimulus; IDA:RGD; .
GOGO:0006096; P:glycolysis; IDA:RGD; .
GOGO:0042866; P:pyruvate biosynthetic process; IDA:RGD; .
GOGO:0033198; P:response to ATP; IDA:RGD; .
GOGO:0051591; P:response to cAMP; IDA:RGD; .
GOGO:0009749; P:response to glucose stimulus; IEP:RGD; .
GOGO:0009408; P:response to heat; IDA:RGD; .
GOGO:0001666; P:response to hypoxia; IEP:RGD; .
GOGO:0010226; P:response to lithium ion; IDA:RGD; .
GOGO:0007584; P:response to nutrient; IEP:RGD; .
GOGO:0014070; P:response to organic cyclic compound; IEP:RGD; .
InterProIPR001697; Pyr_Knase; .
InterProIPR015813; Pyrv/PenolPyrv_Kinase; .
InterProIPR011037; Pyrv_Knase-like_insert_dom; .
InterProIPR015794; Pyrv_Knase_a/b; .
InterProIPR018209; Pyrv_Knase_AS; .
InterProIPR015793; Pyrv_Knase_brl; .
InterProIPR015795; Pyrv_Knase_C; .
InterProIPR015806; Pyrv_Knase_insert_dom; .
Gene3DG3DSA:; PK_B_barrel_like; 1; .
Gene3DG3DSA:; Pyrv/PenolPyrv_Kinase_cat; 2; .
Gene3DG3DSA:3.40.1380.20; Pyrv_Knase_a/b; 1; .
KOK12406; -; .
PANTHERPTHR11817; Pyruvate_kinase; 1; .
PfamPF00224; PK; 1; .
PfamPF02887; PK_C; 1; .
SUPFAMSSF50800; PK_B_barrel_like; 1; .
SUPFAMSSF52935; Pyruvate_kinase; 1; .
SUPFAMSSF51621; Pyrv/PenolPyrv_Kinase_cat; 1; .
TIGRFAMsTIGR01064; Pyruv_kin; 1; .

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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server