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Searching in 'World-2DPAGE Repository [0022]' for entry matching: P15429




World-2DPAGE Repository (0022):  P15429

P15429


General information about the entry
View entry in simple text format
Entry nameENOB_RAT
Primary accession numberP15429
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Beta-enolase; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase; AltName: Full=Enolase 3; AltName: Full=Muscle-specific enolase; Short=MSE; AltName: Full=Skeletal muscle enolase;.
Gene nameName=Eno3
Synonyms=Eno-3
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info 		 
HCR_LCR_HEART

MAP LOCATIONS:
pI=7.08; Mw=47326  [identification data]
pI=7.08; Mw=47326  [identification data]
pI=7.08; Mw=47326  [identification data]
pI=7.08; Mw=47326  [identification data]

IDENTIFICATION: SPOT 49: SeqCov=29%. Peptides MS=13/20. Mascot PMF Score=94 [1]
SPOT 288: SeqCov=20%. Peptides MS=8/20. Mascot PMF Score=57 [1]; SPOT 499: SeqCov=27.4%. Peptides MS=11/20. Mascot PMF Score=87 [1]; SPOT 574: SeqCov=31.1%. Peptides MS=11/20. Mascot PMF Score=96 [1].
PEPTIDE EVIDENCE: SPOT 49: 127-GVPLYR-132 (13) / 33-AAVPSGASTGIYEALELR-50 (65) [1]
SPOT 288: 33-AAVPSGASTGIYEALELR-50 (70) [1]; SPOT 499: 127-GVPLYR-132 (18) / 240-VVIGMDVAASEFYR-253 (31) / 33-AAVPSGASTGIYEALELR-50 (79) [1]; SPOT 574: 33-AAVPSGASTGIYEALELR-50 (44) [1].
MAPPING (identification):
SPOT 49: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 288: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 499: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 574: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtP15429; ENOB_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameENOB_RAT
Primary accession numberP15429
Secondary accession number(s) Q5XIV3
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on November 16, 2011 (version 105)
Name and origin of the protein
DescriptionRecName: Full=Beta-enolase; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase; AltName: Full=Enolase 3; AltName: Full=Muscle-specific enolase; Short=MSE; AltName: Full=Skeletal muscle enolase;
Gene nameName=Eno3
Synonyms=Eno-3
Encoded onName=Eno3; Synonyms=Eno-3
KeywordsComplete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Lyase; Magnesium; Metal-binding; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLY00979; CAA68788.1; -; mRNA
EMBLBC083566; AAH83566.1; -; mRNA
EMBLX57774; CAA40920.1; -; Genomic_DNA
IPIIPI00231631; -; .
PIRS02072; S02072; .
RefSeqNP_037081.2; NM_012949.2; .
UniGeneRn.3443; -; .
ProteinModelPortalP15429; -; .
SMRP15429; 2-431; .
STRINGP15429; -; .
PhosphoSiteP15429; -; .
PRIDEP15429; -; .
EnsemblENSRNOT00000005612; ENSRNOP00000005612; ENSRNOG00000004078; .
GeneID25438; -; .
KEGGrno:25438; -; .
UCSCNM_012949; rat; .
CTD2027; -; .
RGD2555; Eno3; .
eggNOGroNOG10078; -; .
GeneTreeENSGT00550000074560; -; .
HOVERGENHBG000067; -; .
InParanoidP15429; -; .
OMAGELYKNF; -; .
OrthoDBEOG4T783B; -; .
PhylomeDBP15429; -; .
NextBio606651; -; .
ArrayExpressP15429; -; .
GenevestigatorP15429; -; .
GermOnlineENSRNOG00000004078; Rattus norvegicus; .
GOGO:0005624; C:membrane fraction; IDA:RGD; .
GOGO:0000015; C:phosphopyruvate hydratase complex; IEA:InterPro; .
GOGO:0000287; F:magnesium ion binding; IEA:InterPro; .
GOGO:0004634; F:phosphopyruvate hydratase activity; IDA:RGD; .
GOGO:0046982; F:protein heterodimerization activity; IDA:RGD; .
GOGO:0042803; F:protein homodimerization activity; IDA:RGD; .
GOGO:0007568; P:aging; IEP:RGD; .
GOGO:0006096; P:glycolysis; IEA:UniProtKB-KW; .
GOGO:0042493; P:response to drug; IEP:RGD; .
GOGO:0043403; P:skeletal muscle tissue regeneration; IEP:RGD; .
InterProIPR000941; Enolase; .
InterProIPR020810; Enolase_C; .
InterProIPR020809; Enolase_CS; .
InterProIPR020811; Enolase_N; .
KOK01689; -; .
PANTHERPTHR11902; Enolase; 1; .
PfamPF00113; Enolase_C; 1; .
PfamPF03952; Enolase_N; 1; .
PIRSFPIRSF001400; Enolase; 1; .
PRINTSPR00148; ENOLASE; .
TIGRFAMsTIGR01060; Eno; 1; .
PROSITEPS00164; ENOLASE; 1; .


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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server