World-2DPAGE Repository
Home
|
Contact
Due to scheduled maintenance work, this service will not be available from Tuesday August 23rd 06:00 pm until Wednesday August 24th 08:00 am
CEST
. Apologies for the inconvenience.
!!This displayer contains script commands. You should enable JavaScripting within your browser preferences, or use another browser!
World-2DPAGE Repository
Search by
[accession number]
[description, ID or gene]
[author names]
[spot ID / serial number]
[identification methods]
[pI / Mw range]
[combined fields]
Maps
[experimental info]
[protein list]
[graphical interface]
Databases
0001
0002
0003
0004
0005
0006
0007
0008
0009
0010
0011
0012
0014
0017
0018
0019
0020
0021
0022
0023
0024
0026
0027
0029
0030
0031
0032
0034
0035
0036
0037
0038
0039
0040
0042
0043
0044
0046
0047
0050
0051
0053
0054
0056
0059
0061
0062
0063
0066
0068
0069
0076
0077
0080
0084
0085
0088
use 'Ctrl' to select several
databases
Select Remote Interfaces
[All Interfaces]
SWISS-2DPAGE
World-2DPAGE Portal
Exclude local DBs
has only effect if a remote
interface is selected
Searching in 'World-2DPAGE Repository [0022]' for entry
matching:
P15429
World-2DPAGE Repository
(0022):
P15429
P15429
General information about the entry
View entry in simple text format
Entry name
ENOB_RAT
Primary accession number
P15429
integrated into World-2DPAGE Repository (0022) on
May 7, 2010 (release 1)
2D Annotations were last modified on
June 22, 2011 (version 2)
General Annotations were last modified on
November 24, 2011 (version 2)
Name and origin of the protein
Description
RecName: Full=Beta-enolase; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase; AltName: Full=Enolase 3; AltName: Full=Muscle-specific enolase; Short=MSE; AltName: Full=Skeletal muscle enolase;.
Gene name
Name=Eno3
Synonyms=Eno-3
Annotated species
Rattus [TaxID:
10114
]
Taxonomy
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]
2D-PAGE GEL CHARACTERIZATION
DOI=
10.1002/pmic.201000593
;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein
HCR_LCR_HEART
{HCR LCR Cardiac Map}
Rattus
Tissue: Heart
map experimental info
HCR_LCR_HEART
MAP LOCATIONS:
SPOT 49
:
pI=7.08; Mw=47326
[identification data]
SPOT 288
:
pI=7.08; Mw=47326
[identification data]
SPOT 499
:
pI=7.08; Mw=47326
[identification data]
SPOT 574
:
pI=7.08; Mw=47326
[identification data]
IDENTIFICATION:
SPOT 49: SeqCov=29%. Peptides MS=13/20. Mascot PMF Score=94 [
1
]
SPOT 288: SeqCov=20%. Peptides MS=8/20. Mascot PMF Score=57 [
1
]; SPOT 499: SeqCov=27.4%. Peptides MS=11/20. Mascot PMF Score=87 [
1
]; SPOT 574: SeqCov=31.1%. Peptides MS=11/20. Mascot PMF Score=96 [
1
].
PEPTIDE EVIDENCE:
SPOT 49: 127-GVPLYR-132 (13) / 33-AAVPSGASTGIYEALELR-50 (65) [
1
]
SPOT 288: 33-AAVPSGASTGIYEALELR-50 (70) [
1
]; SPOT 499: 127-GVPLYR-132 (18) / 240-VVIGMDVAASEFYR-253 (31) / 33-AAVPSGASTGIYEALELR-50 (79) [
1
]; SPOT 574: 33-AAVPSGASTGIYEALELR-50 (44) [
1
].
MAPPING (identification):
SPOT
49
:
Peptide mass fingerprinting
[
1
];
Tandem mass spectrometry
[
1
];
SPOT
288
:
Peptide mass fingerprinting
[
1
];
Tandem mass spectrometry
[
1
];
SPOT
499
:
Peptide mass fingerprinting
[
1
];
Tandem mass spectrometry
[
1
];
SPOT
574
:
Peptide mass fingerprinting
[
1
];
Tandem mass spectrometry
[
1
].
Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-Prot
P15429; ENOB_RAT.
2D PAGE maps for identified proteins
How to interpret a protein map
You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
Warning 1
: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
Warning 2
: the 2D PAGE map is built on demand. This may take some few seconds to be computed.
External data extracted from
UniProtKB/Swiss-Prot
Extracted from
UniProtKB/Swiss-Prot
, release:
2011_11
Entry name
ENOB_RAT
Primary accession number
P15429
Secondary accession number(s)
Q5XIV3
Sequence was last modified on
January 23, 2007 (version 3)
Annotations were last modified on
November 16, 2011 (version 105)
Name and origin of the protein
Description
RecName: Full=Beta-enolase; EC=4.2.1.11; AltName: Full=2-phospho-D-glycerate hydro-lyase; AltName: Full=Enolase 3; AltName: Full=Muscle-specific enolase; Short=MSE; AltName: Full=Skeletal muscle enolase;
Gene name
Name=Eno3
Synonyms=Eno-3
Encoded on
Name=Eno3; Synonyms=Eno-3
Keywords
Complete proteome
;
Cytoplasm
;
Direct protein sequencing
;
Glycolysis
;
Lyase
;
Magnesium
;
Metal-binding
;
Reference proteome
.
Copyright
Copyrighted by the UniProt Consortium, see
https://www.uniprot.org/help/license
. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL
Y00979; CAA68788.1
; -; mRNA
EMBL
BC083566; AAH83566.1
; -; mRNA
EMBL
X57774; CAA40920.1
; -; Genomic_DNA
IPI
IPI00231631; -
; .
PIR
S02072; S02072
; .
RefSeq
NP_037081.2; NM_012949.2
; .
UniGene
Rn.3443; -
; .
ProteinModelPortal
P15429; -
; .
SMR
P15429; 2-431
; .
STRING
P15429; -
; .
PhosphoSite
P15429; -
; .
PRIDE
P15429; -
; .
Ensembl
ENSRNOT00000005612; ENSRNOP00000005612
; ENSRNOG00000004078; .
GeneID
25438; -
; .
KEGG
rno:25438; -
; .
UCSC
NM_012949; rat
; .
CTD
2027; -
; .
RGD
2555; Eno3
; .
eggNOG
roNOG10078; -
; .
GeneTree
ENSGT00550000074560; -
; .
HOVERGEN
HBG000067; -
; .
InParanoid
P15429; -
; .
OMA
GELYKNF; -
; .
OrthoDB
EOG4T783B; -
; .
PhylomeDB
P15429; -
; .
NextBio
606651; -
; .
ArrayExpress
P15429; -
; .
Genevestigator
P15429; -
; .
GermOnline
ENSRNOG00000004078; Rattus norvegicus
; .
GO
GO:0005624; C:membrane fraction
; IDA:RGD; .
GO
GO:0000015; C:phosphopyruvate hydratase complex
; IEA:InterPro; .
GO
GO:0000287; F:magnesium ion binding
; IEA:InterPro; .
GO
GO:0004634; F:phosphopyruvate hydratase activity
; IDA:RGD; .
GO
GO:0046982; F:protein heterodimerization activity
; IDA:RGD; .
GO
GO:0042803; F:protein homodimerization activity
; IDA:RGD; .
GO
GO:0007568; P:aging
; IEP:RGD; .
GO
GO:0006096; P:glycolysis
; IEA:UniProtKB-KW; .
GO
GO:0042493; P:response to drug
; IEP:RGD; .
GO
GO:0043403; P:skeletal muscle tissue regeneration
; IEP:RGD; .
InterPro
IPR000941; Enolase
; .
InterPro
IPR020810; Enolase_C
; .
InterPro
IPR020809; Enolase_CS
; .
InterPro
IPR020811; Enolase_N
; .
KO
K01689; -
; .
PANTHER
PTHR11902; Enolase
; 1; .
Pfam
PF00113; Enolase_C
; 1; .
Pfam
PF03952; Enolase_N
; 1; .
PIRSF
PIRSF001400; Enolase
; 1; .
PRINTS
PR00148; ENOLASE
; .
TIGRFAMs
TIGR01060; Eno
; 1; .
PROSITE
PS00164; ENOLASE
; 1; .
World-2DPAGE Repository (search AC)
Database constructed and maintained by
SIB
, using the
Make2D-DB II
package (
ver. 3.10.2
) from the
World-2DPAGE Constellation
of the
ExPASy web server