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Searching in 'World-2DPAGE Repository [0030]' for entry matching: P22734




World-2DPAGE Repository (0030):  P22734


P22734


General information about the entry
View entry in simple text format
Entry nameCOMT_RAT
Primary accession numberP22734
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Catechol O-methyltransferase; EC=2.1.1.6;.
Gene nameName=Comt
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info
 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=4.67; Mw=25054  [identification data]
pI=4.81; Mw=24303  [identification data]

IDENTIFICATION: SPOT 2261: Mascot protein score=65. Number of peptides=3 [1]
SPOT 2296: Mascot protein score=321. Number of peptides=7 [1].
MAPPING (identification):
SPOT 2261: Tandem mass spectrometry [1];
SPOT 2296: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtP22734; COMT_RAT.
World-2DPAGE RepositoryP22734; COMT_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameCOMT_RAT
Primary accession numberP22734
Sequence was last modified on May 1, 1992 (version 2)
Annotations were last modified on November 16, 2011 (version 127)
Name and origin of the protein
DescriptionRecName: Full=Catechol O-methyltransferase; EC=2.1.1.6;
Gene nameName=Comt
Encoded onName=Comt
Keywords3D-structure; Alternative initiation; Catecholamine metabolism; Cell membrane; Complete proteome; Cytoplasm; Magnesium; Membrane; Metal-binding; Methyltransferase; Neurotransmitter degradation; Phosphoprotein; Reference proteome; S-adenosyl-L-methionine; Signal-anchor; Transferase; Transmembrane; Transmembrane helix.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLZ12651; CAA78276.1; -; Genomic_DNA
EMBLM60754; AAA40882.1; ALT_INIT; Genomic_DNA
EMBLBC081850; AAH81850.1; -; mRNA
EMBLM60753; AAA40881.1; ALT_INIT; mRNA
IPIIPI00210280; -; .
IPIIPI00760117; -; .
PIRS22090; S22090; .
RefSeqNP_036663.1; NM_012531.2; .
UniGeneRn.220; -; .
PDB1H1D; X-ray; 2.00 A; A=44-264
PDB1JR4; X-ray; 2.63 A; A=44-264
PDB1VID; X-ray; 2.00 A; A=44-264
PDB2CL5; X-ray; 1.60 A; A/B=44-264
PDB2ZLB; X-ray; 2.20 A; A=44-264
PDB2ZTH; X-ray; 2.60 A; A=44-264
PDB2ZVJ; X-ray; 2.30 A; A=44-264
PDB3A7D; X-ray; 2.40 A; A=44-264
PDB3HVH; X-ray; 1.30 A; A=44-264
PDB3HVI; X-ray; 1.20 A; A=44-264
PDB3HVJ; X-ray; 1.79 A; A/B=44-264
PDB3HVK; X-ray; 1.30 A; A=44-264
PDB3NW9; X-ray; 1.65 A; A=44-264
PDB3NWB; X-ray; 1.30 A; A=44-264
PDB3NWE; X-ray; 1.50 A; A=44-264
PDB3OE4; X-ray; 1.49 A; A=44-264
PDB3OE5; X-ray; 1.52 A; A=44-264
PDB3OZR; X-ray; 1.73 A; A=44-264
PDB3OZS; X-ray; 1.44 A; A=44-264
PDB3OZT; X-ray; 1.48 A; A=44-264
PDBsum1H1D; -; .
PDBsum1JR4; -; .
PDBsum1VID; -; .
PDBsum2CL5; -; .
PDBsum2ZLB; -; .
PDBsum2ZTH; -; .
PDBsum2ZVJ; -; .
PDBsum3A7D; -; .
PDBsum3HVH; -; .
PDBsum3HVI; -; .
PDBsum3HVJ; -; .
PDBsum3HVK; -; .
PDBsum3NW9; -; .
PDBsum3NWB; -; .
PDBsum3NWE; -; .
PDBsum3OE4; -; .
PDBsum3OE5; -; .
PDBsum3OZR; -; .
PDBsum3OZS; -; .
PDBsum3OZT; -; .
ProteinModelPortalP22734; -; .
SMRP22734; 45-258; .
STRINGP22734; -; .
PhosphoSiteP22734; -; .
PRIDEP22734; -; .
EnsemblENSRNOT00000050269; ENSRNOP00000043148; ENSRNOG00000001889; .
GeneID24267; -; .
KEGGrno:24267; -; .
UCSCBC081850; rat; .
CTD1312; -; .
RGD2379; Comt; .
eggNOGroNOG14769; -; .
GeneTreeENSGT00390000011316; -; .
HOVERGENHBG005376; -; .
InParanoidP22734; -; .
OMASRFECTH; -; .
OrthoDBEOG4G1MH8; -; .
PhylomeDBP22734; -; .
BioCycMetaCyc:MONOMER-15100; -; .
NextBio602825; -; .
ArrayExpressP22734; -; .
GenevestigatorP22734; -; .
GermOnlineENSRNOG00000001889; Rattus norvegicus; .
GOGO:0016021; C:integral to membrane; IEA:UniProtKB-KW; .
GOGO:0005624; C:membrane fraction; IDA:RGD; .
GOGO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0016206; F:catechol O-methyltransferase activity; IDA:RGD; .
GOGO:0000287; F:magnesium ion binding; TAS:RGD; .
GOGO:0008210; P:estrogen metabolic process; IMP:RGD; .
GOGO:0007565; P:female pregnancy; IMP:RGD; .
GOGO:0007612; P:learning; IMP:RGD; .
GOGO:0048609; P:multicellular organismal reproductive process; IMP:RGD; .
GOGO:0045963; P:negative regulation of dopamine metabolic process; IMP:RGD; .
GOGO:0048662; P:negative regulation of smooth muscle cell proliferation; IMP:RGD; .
GOGO:0042135; P:neurotransmitter catabolic process; IEA:UniProtKB-KW; .
GOGO:0050668; P:positive regulation of homocysteine metabolic process; IMP:RGD; .
GOGO:0042493; P:response to drug; IEP:RGD; .
GOGO:0032496; P:response to lipopolysaccharide; IEP:RGD; .
GOGO:0014070; P:response to organic cyclic compound; IEP:RGD; .
GOGO:0048265; P:response to pain; IMP:RGD; .
GOGO:0046498; P:S-adenosylhomocysteine metabolic process; TAS:RGD; .
GOGO:0046500; P:S-adenosylmethionine metabolic process; TAS:RGD; .
InterProIPR017128; Catechol_O-MeTrfase_euk; .
InterProIPR002935; O-MeTrfase_3; .
KOK00545; -; .
PANTHERPTHR10509; Methyltransf_3; 1; .
PfamPF01596; Methyltransf_3; 1; .
PIRSFPIRSF037177; Catechol_O-mtfrase_euk; 1; .



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