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Searching in 'World-2DPAGE Repository [0022]' for entry matching: P23928




World-2DPAGE Repository (0022):  P23928


P23928


General information about the entry
View entry in simple text format
Entry nameCRYAB_RAT
Primary accession numberP23928
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Alpha-crystallin B chain; AltName: Full=Alpha(B)-crystallin;.
Gene nameName=Cryab
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info
 
HCR_LCR_HEART

MAP LOCATIONS:
pI=6.76; Mw=20076  [identification data]
pI=6.76; Mw=20076  [identification data]
pI=6.76; Mw=20076  [identification data]

IDENTIFICATION: SPOT 20: SeqCov=26.9%. Peptides MS=6/20. Mascot PMF Score=44 [1]
SPOT 640: SeqCov=47.4%. Peptides MS=9/20. Mascot PMF Score=78 [1]; SPOT 717: SeqCov=47.4%. Peptides MS=9/20. Mascot PMF Score=91 [1].
PEPTIDE EVIDENCE: SPOT 20: 158-TIPITR-163 (12) / 108-QDEHGFISR-116 (54) / 12-RPFFPFHSPSR-22 (42) [1]
SPOT 640: 158-TIPITR-163 (15) / 108-QDEHGFISR-116 (60) / 12-RPFFPFHSPSR-22 (81) [1]; SPOT 717: 158-TIPITR-163 (31) / 108-QDEHGFISR-116 (60) / 12-RPFFPFHSPSR-22 (80) [1].
MAPPING (identification):
SPOT 20: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 640: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 717: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtP23928; CRYAB_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameCRYAB_RAT
Primary accession numberP23928
Secondary accession number(s) Q6LDR2
Sequence was last modified on March 1, 1992 (version 1)
Annotations were last modified on November 16, 2011 (version 103)
Name and origin of the protein
DescriptionRecName: Full=Alpha-crystallin B chain; AltName: Full=Alpha(B)-crystallin;
Gene nameName=Cryab
Encoded onName=Cryab
KeywordsAcetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Eye lens protein; Glycoprotein; Methylation; Nucleus; Oxidation; Phosphoprotein; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLS74229; AAB20759.1; -; mRNA
EMBLS77138; AAP31995.1; -; mRNA
EMBLM55534; AAA40977.1; -; mRNA
EMBLX60352; CAA42911.1; ALT_INIT; mRNA
EMBLX60351; CAA42910.1; -; mRNA
EMBLS77142; -; NOT_ANNOTATED_CDS; mRNA
EMBLU04320; AAA03655.1; -; Unassigned_DNA
EMBLM24092; AAA40978.1; -; mRNA
IPIIPI00215465; -; .
PIRA23681; A23681; .
RefSeqNP_037067.1; NM_012935.3; .
UniGeneRn.98208; -; .
ProteinModelPortalP23928; -; .
IntActP23928; 1; .
MINTMINT-6801317; -; .
STRINGP23928; -; .
PhosphoSiteP23928; -; .
Rat-heart-2DPAGEP23928; -; .
PRIDEP23928; -; .
EnsemblENSRNOT00000059127; ENSRNOP00000055901; ENSRNOG00000010524; .
GeneID25420; -; .
KEGGrno:25420; -; .
UCSCM55534; rat; .
CTD1410; -; .
RGD2414; Cryab; .
eggNOGroNOG11002; -; .
GeneTreeENSGT00550000074302; -; .
HOVERGENHBG054766; -; .
OrthoDBEOG4G1MHK; -; .
PhylomeDBP23928; -; .
NextBio606579; -; .
ArrayExpressP23928; -; .
GenevestigatorP23928; -; .
GermOnlineENSRNOG00000010524; Rattus norvegicus; .
GOGO:0032432; C:actin filament bundle; IDA:RGD; .
GOGO:0009986; C:cell surface; IDA:RGD; .
GOGO:0005794; C:Golgi apparatus; IDA:RGD; .
GOGO:0015630; C:microtubule cytoskeleton; IDA:RGD; .
GOGO:0005634; C:nucleus; ISS:UniProtKB; .
GOGO:0008017; F:microtubule binding; IDA:RGD; .
GOGO:0042803; F:protein homodimerization activity; ISS:UniProtKB; .
GOGO:0005212; F:structural constituent of eye lens; TAS:RGD; .
GOGO:0007568; P:aging; IEP:RGD; .
GOGO:0006006; P:glucose metabolic process; IMP:RGD; .
GOGO:0031109; P:microtubule polymerization or depolymerization; IMP:RGD; .
GOGO:0030308; P:negative regulation of cell growth; IDA:RGD; .
GOGO:2000378; P:negative regulation of reactive oxygen species metabolic process; IDA:RGD; .
GOGO:0006457; P:protein folding; IDA:RGD; .
GOGO:0032355; P:response to estradiol stimulus; IEP:RGD; .
GOGO:0042542; P:response to hydrogen peroxide; IEP:RGD; .
GOGO:0051403; P:stress-activated MAPK cascade; IMP:RGD; .
InterProIPR001436; Alpha-crystallin/HSP; .
InterProIPR012273; Alpha-crystallin_B; .
InterProIPR003090; Alpha-crystallin_N; .
InterProIPR002068; Hsp20; .
InterProIPR008978; HSP20-like_chaperone; .
KOK09542; -; .
PANTHERPTHR11527:SF37; A-crystallin_B; 1; .
PfamPF00525; Crystallin; 1; .
PfamPF00011; HSP20; 1; .
PIRSFPIRSF036514; Sm_HSP_B1; 1; .
PRINTSPR00299; ACRYSTALLIN; .
SUPFAMSSF49764; HSP20_chap; 1; .
PROSITEPS01031; HSP20; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server