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Searching in 'World-2DPAGE Repository [0030]' for entry matching: P28841




World-2DPAGE Repository (0030):  P28841


P28841


General information about the entry
View entry in simple text format
Entry nameNEC2_RAT
Primary accession numberP28841
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Neuroendocrine convertase 2; Short=NEC 2; EC=3.4.21.94; AltName: Full=KEX2-like endoprotease 2; AltName: Full=Prohormone convertase 2; AltName: Full=Proprotein convertase 2; Short=PC2; Flags: Precursor;.
Gene nameName=Pcsk2
Synonyms=Nec-2, Nec2, Rpc2
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info
 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=4.99; Mw=76087  [identification data]
pI=4.85; Mw=76721  [identification data]
pI=4.92; Mw=76298  [identification data]
pI=5.07; Mw=75877  [identification data]
pI=4.78; Mw=74421  [identification data]
pI=4.85; Mw=74011  [identification data]
pI=4.92; Mw=73501  [identification data]

IDENTIFICATION: SPOT 610: Mascot protein score=168. Number of peptides=5 [1]
SPOT 624: Mascot protein score=70. Number of peptides=4 [1]; SPOT 625: Mascot protein score=78. Number of peptides=4 [1]; SPOT 632: Mascot protein score=66. Number of peptides=4 [1]; SPOT 661: Mascot protein score=206. Number of peptides=6 [1]; SPOT 680: Mascot protein score=201. Number of peptides=7 [1]; SPOT 694: Mascot protein score=113. Number of peptides=3 [1].
MAPPING (identification):
SPOT 610: Tandem mass spectrometry [1];
SPOT 624: Tandem mass spectrometry [1];
SPOT 625: Tandem mass spectrometry [1];
SPOT 632: Tandem mass spectrometry [1];
SPOT 661: Tandem mass spectrometry [1];
SPOT 680: Tandem mass spectrometry [1];
SPOT 694: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtP28841; NEC2_RAT.
World-2DPAGE RepositoryP28841; NEC2_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameNEC2_RAT
Primary accession numberP28841
Sequence was last modified on December 1, 1992 (version 1)
Annotations were last modified on November 16, 2011 (version 99)
Name and origin of the protein
DescriptionRecName: Full=Neuroendocrine convertase 2; Short=NEC 2; EC=3.4.21.94; AltName: Full=KEX2-like endoprotease 2; AltName: Full=Prohormone convertase 2; AltName: Full=Proprotein convertase 2; Short=PC2; Flags: Precursor;
Gene nameName=Pcsk2
Synonyms=Nec-2, Nec2, Rpc2
Encoded onName=Pcsk2; Synonyms=Nec-2, Nec2, Rpc2
KeywordsCleavage on pair of basic residues; Complete proteome; Cytoplasmic vesicle; Disulfide bond; Glycoprotein; Hydrolase; Protease; Reference proteome; Serine protease; Signal; Zymogen.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM76706; AAA40946.1; -; mRNA
EMBLM83746; AAA41477.1; -; mRNA
IPIIPI00204864; -; .
PIRB41556; KXRTC2; .
RefSeqNP_036878.1; NM_012746.1; .
UniGeneRn.89052; -; .
ProteinModelPortalP28841; -; .
SMRP28841; 119-596; .
IntActP28841; 1; .
STRINGP28841; -; .
MEROPSS08.073; -; .
PRIDEP28841; -; .
EnsemblENSRNOT00000007249; ENSRNOP00000007249; ENSRNOG00000005438; .
GeneID25121; -; .
KEGGrno:25121; -; .
UCSCNM_012746; rat; .
CTD5126; -; .
RGD3273; Pcsk2; .
eggNOGroNOG07042; -; .
GeneTreeENSGT00600000084064; -; .
HOVERGENHBG008705; -; .
InParanoidP28841; -; .
OMANYNADAS; -; .
OrthoDBEOG46MBJ1; -; .
PhylomeDBP28841; -; .
NextBio605503; -; .
ArrayExpressP28841; -; .
GenevestigatorP28841; -; .
GermOnlineENSRNOG00000005438; Rattus norvegicus; .
GOGO:0005615; C:extracellular space; IBA:RefGenome; .
GOGO:0030133; C:transport vesicle; IEA:UniProtKB-SubCell; .
GOGO:0005515; F:protein binding; IPI:IntAct; .
GOGO:0004252; F:serine-type endopeptidase activity; IMP:BHF-UCL; .
GOGO:0034231; P:islet amyloid polypeptide processing; IMP:BHF-UCL; .
GOGO:0006508; P:proteolysis; IMP:RGD; .
InterProIPR008979; Galactose-bd-like; .
InterProIPR000209; Peptidase_S8/S53; .
InterProIPR023827; Peptidase_S8_Asp-AS; .
InterProIPR022398; Peptidase_S8_His-AS; .
InterProIPR023828; Peptidase_S8_Ser-AS; .
InterProIPR015500; Peptidase_S8_subtilisin-rel; .
InterProIPR009020; Prot_inh_propept; .
InterProIPR002884; PrprotnconvertsP; .
Gene3DG3DSA:3.40.50.200; Pept_S8_S53; 1; .
KOK01360; -; .
PANTHERPTHR10795; SubtilSerProt; 1; .
PfamPF01483; P_proprotein; 1; .
PfamPF00082; Peptidase_S8; 1; .
PRINTSPR00723; SUBTILISIN; .
SUPFAMSSF49785; Gal_bind_like; 1; .
SUPFAMSSF52743; Pept_S8_S53; 1; .
SUPFAMSSF54897; Prot_inh_propept; 1; .
PROSITEPS00136; SUBTILASE_ASP; 1; .
PROSITEPS00137; SUBTILASE_HIS; 1; .
PROSITEPS00138; SUBTILASE_SER; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server