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World-2DPAGE Repository

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Searching in 'World-2DPAGE Repository [0022]' for entry matching: P42123

World-2DPAGE Repository (0022):  P42123


General information about the entry
View entry in simple text format
Entry nameLDHB_RAT
Primary accession numberP42123
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=L-lactate dehydrogenase B chain; Short=LDH-B; EC=; AltName: Full=LDH heart subunit; Short=LDH-H;.
Gene nameName=Ldhb
Synonyms=Ldh-2, Ldh2
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

Tissue: Heart
  map experimental info

pI=5.70; Mw=36874  [identification data]
pI=5.70; Mw=36874  [identification data]
pI=5.70; Mw=36874  [identification data]

IDENTIFICATION: SPOT 176: SeqCov=29.9%. Peptides MS=9/20. Mascot PMF Score=74 [1]
SPOT 477: SeqCov=47%. Peptides MS=14/20. Mascot PMF Score=155 [1]; SPOT 538: SeqCov=35.6%. Peptides MS=10/20. Mascot PMF Score=89 [1].
PEPTIDE EVIDENCE: SPOT 176: 108-LNLVQR-113 (20) / 92-IVVVTAGVR-100 (25) / 280-GMYGIENEVFLSLPCILNAR-299 (83) [1]
SPOT 538: 108-LNLVQR-113 (34) / 92-IVVVTAGVR-100 (43) / 280-GMYGIENEVFLSLPCILNAR-299 (79) [1].
MAPPING (identification):
SPOT 176: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 477: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 538: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
UniProtKB/Swiss-ProtP42123; LDHB_RAT.

2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameLDHB_RAT
Primary accession numberP42123
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on November 16, 2011 (version 99)
Name and origin of the protein
DescriptionRecName: Full=L-lactate dehydrogenase B chain; Short=LDH-B; EC=; AltName: Full=LDH heart subunit; Short=LDH-H;
Gene nameName=Ldhb
Synonyms=Ldh-2, Ldh2
Encoded onName=Ldhb; Synonyms=Ldh-2, Ldh2
KeywordsAcetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; NAD; Oxidoreductase; Phosphoprotein; Reference proteome.
Copyrighted by the UniProt Consortium, see Distributed under the Creative Commons Attribution-NoDerivs License
EMBLU07181; AAA50439.1; -; mRNA
EMBLBC059149; AAH59149.1; -; mRNA
IPIIPI00231783; -; .
PIRI62761; I62761; .
RefSeqNP_036727.1; NM_012595.1; .
UniGeneRn.1785; -; .
ProteinModelPortalP42123; -; .
SMRP42123; 2-333; .
IntActP42123; 1; .
STRINGP42123; -; .
PhosphoSiteP42123; -; .
World-2DPAGE0004:P42123; -; .
PRIDEP42123; -; .
EnsemblENSRNOT00000017965; ENSRNOP00000017965; ENSRNOG00000013000; .
GeneID24534; -; .
KEGGrno:24534; -; .
NMPDRfig|10116.3.peg.22502; -; .
UCSCNM_012595; rat; .
CTD3945; -; .
RGD2997; Ldhb; .
eggNOGmaNOG09588; -; .
GeneTreeENSGT00550000074541; -; .
HOVERGENHBG000462; -; .
InParanoidP42123; -; .
OrthoDBEOG4RR6HR; -; .
PhylomeDBP42123; -; .
NextBio603604; -; .
ArrayExpressP42123; -; .
GenevestigatorP42123; -; .
GermOnlineENSRNOG00000013000; Rattus norvegicus; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0042802; F:identical protein binding; IDA:RGD; .
GOGO:0019900; F:kinase binding; IPI:RGD; .
GOGO:0004459; F:L-lactate dehydrogenase activity; IDA:RGD; .
GOGO:0051287; F:NAD binding; IDA:RGD; .
GOGO:0006096; P:glycolysis; IEA:UniProtKB-KW; .
GOGO:0006089; P:lactate metabolic process; IDA:RGD; .
GOGO:0019674; P:NAD metabolic process; IDA:RGD; .
InterProIPR001557; L-lactate/malate_DH; .
InterProIPR011304; L-lactate_DH; .
InterProIPR018177; L-lactate_DH_AS; .
InterProIPR022383; Lactate/malate_DH_C; .
InterProIPR001236; Lactate/malate_DH_N; .
InterProIPR015955; Lactate_DH/Glyco_Ohase_4_C; .
InterProIPR016040; NAD(P)-bd_dom; .
Gene3DG3DSA:; lact_mal_DH; 1; .
Gene3DG3DSA:; NAD(P)-bd; 1; .
KOK00016; -; .
PfamPF02866; Ldh_1_C; 1; .
PfamPF00056; Ldh_1_N; 1; .
PIRSFPIRSF000102; Lac_mal_DH; 1; .
SUPFAMSSF56327; Lactate_DH/Glyco_hydro_4_C; 1; .
PROSITEPS00064; L_LDH; 1; .

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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server