World-2DPAGE Home
Home  |  Contact
Due to maintenance work, this service and ftp://ftp.expasy.org will be unavailable from Tuesday August 23rd 18:00 until Wednesday August 24th 08:00 CEST. Apologies for the inconvenience.

World-2DPAGE Repository 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 
Maps  [experimental info] 
[protein list] 
[graphical interface]
     Databases

use 'Ctrl' to select several
databases

Select Remote Interfaces
[All Interfaces]
SWISS-2DPAGE
World-2DPAGE Portal
Exclude local DBs
has only effect if a remote
interface is selected
     
Searching in 'World-2DPAGE Repository [0030]' for entry matching: P42669




World-2DPAGE Repository (0030):  P42669

P42669


General information about the entry
View entry in simple text format
Entry namePURA_MOUSE
Primary accession numberP42669
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Transcriptional activator protein Pur-alpha; AltName: Full=Purine-rich single-stranded DNA-binding protein alpha;.
Gene nameName=Pura
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info 		 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=6.36; Mw=50382  [identification data]
pI=6.32; Mw=49415  [identification data]

IDENTIFICATION: SPOT 1368: Mascot protein score=65. Number of peptides=2 [1]
SPOT 1397: Mascot protein score=46. Number of peptides=2 [1].
MAPPING (identification):
SPOT 1368: Tandem mass spectrometry [1];
SPOT 1397: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UCD-2DPAGEP42669; PURA_MOUSE.
UniProtKB/Swiss-ProtP42669; PURA_MOUSE.
World-2DPAGE RepositoryP42669; PURA_MOUSE.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry namePURA_MOUSE
Primary accession numberP42669
Sequence was last modified on November 1, 1995 (version 1)
Annotations were last modified on September 21, 2011 (version 91)
Name and origin of the protein
DescriptionRecName: Full=Transcriptional activator protein Pur-alpha; AltName: Full=Purine-rich single-stranded DNA-binding protein alpha;
Gene nameName=Pura
Encoded onName=Pura
KeywordsAcetylation; Activator; Complete proteome; Direct protein sequencing; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLU02098; AAA64630.1; -; mRNA
EMBLAF017631; AAB71860.1; -; mRNA
IPIIPI00118447; -; .
RefSeqNP_033015.1; NM_008989.3; .
UniGeneMm.231802; -; .
UniGeneMm.482241; -; .
ProteinModelPortalP42669; -; .
SMRP42669; 57-277; .
IntActP42669; 1; .
STRINGP42669; -; .
PhosphoSiteP42669; -; .
UCD-2DPAGEP42669; -; .
PRIDEP42669; -; .
EnsemblENSMUST00000051301; ENSMUSP00000059404; ENSMUSG00000043991; .
GeneID19290; -; .
KEGGmmu:19290; -; .
UCSCuc008enh.2; mouse; .
CTD5813; -; .
MGIMGI:103079; Pura; .
eggNOGroNOG10448; -; .
HOGENOMHBG383187; -; .
HOVERGENHBG006888; -; .
InParanoidP42669; -; .
OMASEVKNNF; -; .
OrthoDBEOG4PRSRB; -; .
PhylomeDBP42669; -; .
NextBio459411; -; .
ArrayExpressP42669; -; .
BgeeP42669; -; .
CleanExMM_PURA; -; .
GenevestigatorP42669; -; .
GermOnlineENSMUSG00000043991; Mus musculus; .
GOGO:0005737; C:cytoplasm; IDA:MGI; .
GOGO:0030425; C:dendrite; IDA:MGI; .
GOGO:0005662; C:DNA replication factor A complex; IDA:UniProtKB; .
GOGO:0043025; C:neuronal cell body; IDA:MGI; .
GOGO:0032422; F:purine-rich negative regulatory element binding; IDA:HGNC; .
GOGO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:MGI; .
GOGO:0003697; F:single-stranded DNA binding; IDA:UniProtKB; .
GOGO:0046332; F:SMAD binding; IDA:MGI; .
GOGO:0008134; F:transcription factor binding; IPI:UniProtKB; .
GOGO:0000900; F:translation repressor activity; nucleic acid binding; IDA:HGNC
GOGO:0006915; P:apoptosis; NAS:UniProtKB; .
GOGO:0030154; P:cell differentiation; NAS:UniProtKB; .
GOGO:0008283; P:cell proliferation; NAS:UniProtKB; .
GOGO:0031575; P:mitotic cell cycle G1/S transition checkpoint; NAS:UniProtKB; .
GOGO:0045892; P:negative regulation of transcription; DNA-dependent; IDA:MGI
GOGO:0007399; P:nervous system development; IMP:MGI; .
GOGO:0008284; P:positive regulation of cell proliferation; IMP:MGI; .
InterProIPR006628; PUR_DNA_RNA-bd; .
PANTHERPTHR12611; PUR_DNA_RNA_bd; 1; .
PfamPF04845; PurA; 1; .
SMARTSM00712; PUR; 3; .


World-2DPAGE Repository image

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server