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Searching in 'World-2DPAGE Repository [0030]' for entry matching: P63018




World-2DPAGE Repository (0030):  P63018


P63018


General information about the entry
View entry in simple text format
Entry nameHSP7C_RAT
Primary accession numberP63018
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Heat shock cognate 71 kDa protein; AltName: Full=Heat shock 70 kDa protein 8;.
Gene nameName=Hspa8
Synonyms=Hsc70, Hsc73
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info
 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=5.08; Mw=89827  [identification data]
pI=4.70; Mw=79421  [identification data]
pI=5.27; Mw=78438  [identification data]
pI=5.34; Mw=77361  [identification data]
pI=4.45; Mw=54818  [identification data]
pI=4.94; Mw=52155  [identification data]
pI=4.76; Mw=51652  [identification data]
pI=6.70; Mw=29990  [identification data]
pI=5.31; Mw=78006  [identification data]

IDENTIFICATION: SPOT 351: Mascot protein score=814. Number of peptides=10 [1]
SPOT 555: Mascot protein score=85. Number of peptides=3 [1]; SPOT 580: Mascot protein score=29. Number of peptides=4 [1]; SPOT 602: Mascot protein score=84. Number of peptides=4 [1]; SPOT 1235: Mascot protein score=50. Number of peptides=3 [1]; SPOT 1315: Mascot protein score=45. Number of peptides=2 [1]; SPOT 1328: Mascot protein score=34. Number of peptides=2 [1]; SPOT 2057: Mascot protein score=217. Number of peptides=7 [1]; SPOT 2991: Mascot protein score=860. Number of peptides=11 [1].
MAPPING (identification):
SPOT 351: Tandem mass spectrometry [1];
SPOT 555: Tandem mass spectrometry [1];
SPOT 580: Tandem mass spectrometry [1];
SPOT 602: Tandem mass spectrometry [1];
SPOT 1235: Tandem mass spectrometry [1];
SPOT 1315: Tandem mass spectrometry [1];
SPOT 1328: Tandem mass spectrometry [1];
SPOT 2057: Tandem mass spectrometry [1];
SPOT 2991: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtP63018; HSP7C_RAT.
World-2DPAGE RepositoryP63018; HSP7C_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameHSP7C_RAT
Primary accession numberP63018
Secondary accession number(s) P08109 P12225 Q4FZY7 Q62373 Q62374 Q62375
Sequence was last modified on August 1, 1988 (version 1)
Annotations were last modified on November 16, 2011 (version 84)
Name and origin of the protein
DescriptionRecName: Full=Heat shock cognate 71 kDa protein; AltName: Full=Heat shock 70 kDa protein 8;
Gene nameName=Hspa8
Synonyms=Hsc70, Hsc73
Encoded onName=Hspa8; Synonyms=Hsc70, Hsc73
Keywords3D-structure; Acetylation; ATP-binding; Chaperone; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleotide-binding; Phosphoprotein; Reference proteome; Repressor; Stress response; Transcription; Transcription regulation; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLY00054; CAA68265.1; -; Genomic_DNA
EMBLM11942; AAA41354.1; -; mRNA
EMBLBC061547; AAH61547.1; -; mRNA
EMBLBC098914; AAH98914.1; -; mRNA
IPIIPI00208205; -; .
PIRS07197; S07197; .
RefSeqNP_077327.1; NM_024351.2; .
UniGeneRn.120392; -; .
UniGeneRn.201298; -; .
PDB1CKR; NMR; -; A=385-543
PDB1Q2G; Model; -; C=385-543
PDB1UD0; X-ray; 3.45 A; A/B/C/D=542-646
PDB2V7Z; X-ray; 3.50 A; A/B=1-543
PDB7HSC; NMR; -; A=385-543
PDBsum1CKR; -; .
PDBsum1Q2G; -; .
PDBsum1UD0; -; .
PDBsum2V7Z; -; .
PDBsum7HSC; -; .
ProteinModelPortalP63018; -; .
SMRP63018; 1-621; .
IntActP63018; 5; .
MINTMINT-2514077; -; .
STRINGP63018; -; .
PhosphoSiteP63018; -; .
World-2DPAGE0004:P63018; -; .
PRIDEP63018; -; .
GeneID24468; -; .
KEGGrno:24468; -; .
UCSCBC061547; rat; .
CTD3312; -; .
RGD621725; Hspa8; .
eggNOGmaNOG11692; -; .
GeneTreeENSGT00550000074467; -; .
HOVERGENHBG051845; -; .
OrthoDBEOG4W6NVK; -; .
PhylomeDBP63018; -; .
NextBio603401; -; .
GenevestigatorP63018; -; .
GOGO:0070062; C:extracellular vesicular exosome; IDA:RGD; .
GOGO:0042470; C:melanosome; IEA:UniProtKB-SubCell; .
GOGO:0043005; C:neuron projection; IDA:RGD; .
GOGO:0043025; C:neuronal cell body; IDA:RGD; .
GOGO:0043234; C:protein complex; IDA:RGD; .
GOGO:0030529; C:ribonucleoprotein complex; ISS:UniProtKB; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0008021; C:synaptic vesicle; IDA:RGD; .
GOGO:0043531; F:ADP binding; IDA:RGD; .
GOGO:0005524; F:ATP binding; IDA:RGD; .
GOGO:0042623; F:ATPase activity; coupled; IDA:RGD
GOGO:0005102; F:receptor binding; IPI:RGD; .
GOGO:0051082; F:unfolded protein binding; IMP:RGD; .
GOGO:0051085; P:chaperone mediated protein folding requiring cofactor; IDA:RGD; .
GOGO:0045892; P:negative regulation of transcription; DNA-dependent; ISS:UniProtKB
GOGO:0006950; P:response to stress; IEA:UniProtKB-KW; .
GOGO:0006351; P:transcription; DNA-dependent; IEA:UniProtKB-KW
InterProIPR018181; Heat_shock_70_CS; .
InterProIPR001023; Hsp70; .
InterProIPR013126; Hsp_70; .
KOK03283; -; .
PANTHERPTHR19375; Hsp70; 1; .
PfamPF00012; HSP70; 1; .
PRINTSPR00301; HEATSHOCK70; .
PROSITEPS00297; HSP70_1; 1; .
PROSITEPS00329; HSP70_2; 1; .
PROSITEPS01036; HSP70_3; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server