resource logo

World-2DPAGE Repository

Attention: World-2DPAGE is no longer maintained.
It will be discontinued on 31-May-2024.

World-2DPAGE Repository no longer accepts submissions.


World-2DPAGE Repository 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]

     Databases

use 'Ctrl' to select several
databases


Select Remote Interfaces
[All Interfaces]
SWISS-2DPAGE
World-2DPAGE Portal

Exclude local DBs
has only effect if a remote
interface is selected
     
Searching in 'World-2DPAGE Repository [0027]' for entry matching: P63260




World-2DPAGE Repository (0027):  P63260


P63260


General information about the entry
View entry in simple text format
Entry nameACTG_MOUSE
Primary accession numberP63260
integrated into World-2DPAGE Repository (0027) on September 30, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Actin, cytoplasmic 2; AltName: Full=Gamma-actin; Contains: RecName: Full=Actin, cytoplasmic 2, N-terminally processed;.
Gene nameName=Actg1
Synonyms=Actg
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/prca.201000103;
Blutke A., Block C., Berendt F., Herbach N., Kemter E., Amann K., Frolich T., Arnold G.J., Wanke R.
''Differential glomerular proteome analysis of two murine nephropathy models at onset of albuminuria''
Proteomics - Clinical Applications 5(6):375-381 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

GIPRDN_4-7 {glomerular protein lysates of a GIPRdn-tg animal}
Mus musculus (Mouse)
GIPRDN_4-7
  map experimental info
 
GIPRDN_4-7

MAP LOCATIONS:
pI=5.17; Mw=42000  [identification data]
pI=5.17; Mw=41000  [identification data]
pI=5.03; Mw=41000  [identification data]
pI=5.52; Mw=38000  [identification data]
pI=5.27; Mw=16000  [identification data]

EXPRESSION:
SPOT 16: Avg ratio GIPRdn-tg vs. control=1.53 (p-value=0.0099) [1];
SPOT 17: Avg ratio GIPRdn-tg vs. control=1.63 (p-value=0.00094) [1];
SPOT 18: Avg ratio GIPRdn-tg vs. control=1.64 (p-value=0.0025) [1];
SPOT 40: Avg ratio GIPRdn-tg vs. control=1.67 (p-value=0.0018) [1].

IDENTIFICATION: SPOT 16: SeqCov=56%. Mascot protein score=773 [1]
SPOT 17: SeqCov=26%. Mascot protein score=207 [1]; SPOT 18: SeqCov=41%. Mascot protein score=600 [1]; SPOT 20: SeqCov=26%. Mascot protein score=272 [1]; SPOT 40: SeqCov=%. Mascot protein score=247 [1].
MAPPING (identification):
SPOT 16: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 17: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 18: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 20: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 40: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Andreas Blutke, Institute of Veterinary Pathology, University Munich, Germany
Cross-references
COMPLUYEAST-2DPAGEP63260; ACTG_MOUSE.
REPRODUCTION-2DPAGEP63260; P63260.
UCD-2DPAGEP63260; ACTG_MOUSE.
UniProtKB/Swiss-ProtP63260; ACTG_MOUSE.
World-2DPAGE RepositoryP63260; ACTG_MOUSE.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameACTG_MOUSE
Primary accession numberP63260
Secondary accession number(s) P02571 P14104 P99022
Sequence was last modified on July 21, 1986 (version 1)
Annotations were last modified on November 16, 2011 (version 78)
Name and origin of the protein
DescriptionRecName: Full=Actin, cytoplasmic 2; AltName: Full=Gamma-actin; Contains: RecName: Full=Actin, cytoplasmic 2, N-terminally processed;
Gene nameName=Actg1
Synonyms=Actg
Encoded onName=Actg1; Synonyms=Actg
KeywordsAcetylation; ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Methylation; Nucleotide-binding; Phosphoprotein; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX13055; CAA31455.1; -; mRNA
EMBLAK076081; BAC36167.1; -; mRNA
EMBLAK087983; BAC40075.1; -; mRNA
EMBLBC003337; AAH03337.1; -; mRNA
EMBLBC021796; AAH21796.1; -; mRNA
EMBLBC023248; AAH23248.1; -; mRNA
EMBLM21495; AAA37168.1; -; mRNA
IPIIPI00874482; -; .
PIRA30243; ATMSG; .
RefSeqNP_033739.1; NM_009609.2; .
UniGeneMm.196173; -; .
UniGeneMm.426706; -; .
UniGeneMm.470166; -; .
ProteinModelPortalP63260; -; .
SMRP63260; 2-375; .
IntActP63260; 24; .
STRINGP63260; -; .
PhosphoSiteP63260; -; .
COMPLUYEAST-2DPAGEP63260; -; .
REPRODUCTION-2DPAGEP63260; -; .
UCD-2DPAGEP63260; -; .
PRIDEP63260; -; .
EnsemblENSMUST00000071555; ENSMUSP00000071486; ENSMUSG00000062825; .
GeneID11465; -; .
KEGGmmu:11465; -; .
CTD71; -; .
MGIMGI:87906; Actg1; .
HOVERGENHBG003771; -; .
OrthoDBEOG41JZC9; -; .
PhylomeDBP63260; -; .
NextBio278792; -; .
PMAP-CutDBP63260; -; .
ArrayExpressP63260; -; .
BgeeP63260; -; .
CleanExMM_ACTG1; -; .
GenevestigatorP63260; -; .
GermOnlineENSMUSG00000062825; Mus musculus; .
GOGO:0043034; C:costamere; TAS:MGI; .
GOGO:0031941; C:filamentous actin; IDA:MGI; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0005200; F:structural constituent of cytoskeleton; IDA:MGI; .
GOGO:0045214; P:sarcomere organization; IDA:MGI; .
InterProIPR004000; Actin-like; .
InterProIPR020902; Actin/actin-like_CS; .
InterProIPR004001; Actin_CS; .
KOK05692; -; .
PANTHERPTHR11937; Actin_like; 1; .
PfamPF00022; Actin; 1; .
PRINTSPR00190; ACTIN; .
SMARTSM00268; ACTIN; 1; .
PROSITEPS00406; ACTINS_1; 1; .
PROSITEPS00432; ACTINS_2; 1; .
PROSITEPS01132; ACTINS_ACT_LIKE; 1; .



World-2DPAGE Repository image

World-2DPAGE Repository (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server