World-2DPAGE Repository

RecName: Full=Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; EC=3.1.3.16; AltName: Full=CAM-PRP catalytic subunit; AltName: Full=Calmodulin-dependent calcineurin A subunit alpha isoform;.

Gene name

Name=Ppp3ca
Synonyms=Calna

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1111/j.1471-4159.2010.06719.x; VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M. ''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome'' J of Neurochemistry 113(6):1577-1588 (2010)

2D PAGE maps for identified proteins

How to interpret a protein

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus

map experimental info

HIPP_SYNAP

MAP LOCATIONS:

SPOT 370:

pI=5.58; Mw=59290

[identification data]

SPOT 1154:

pI=5.58; Mw=59290

[identification data]

IDENTIFICATION: SPOT 370: Peptides number=8. SeqCov=15.4%. MOWSE score=5.97E+7 [1]
SPOT 1154: Peptides number=8. SeqCov=15.5%. MOWSE score=2.66E+6 [1].
MAPPING (identification):

SPOT 370: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 1154: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Willard Freeman, Penn State College of Medicine, USA

Cross-references

UniProtKB/Swiss-Prot

P63329; PP2BA_RAT.

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	PP2BA_RAT
Primary accession number	P63329
Secondary accession number(s)	P12816 P20652 Q6LDJ8 Q9WUV7 Q9Z1I5
Sequence was last modified on	October 11, 2004 (version 1)
Annotations were last modified on	November 16, 2011 (version 69)
Name and origin of the protein
Description	RecName: Full=Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; EC=3.1.3.16; AltName: Full=CAM-PRP catalytic subunit; AltName: Full=Calmodulin-dependent calcineurin A subunit alpha isoform;
Gene name	Name=Ppp3ca Synonyms=Calna
Encoded on	Name=Ppp3ca; Synonyms=Calna
Keywords	Alternative splicing; Calmodulin-binding; Complete proteome; Direct protein sequencing; Hydrolase; Iron; Metal-binding; Nucleus; Phosphoprotein; Protein phosphatase; Reference proteome; Zinc.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	D90035; BAA14083.1; -; mRNA
EMBL	M29275; AAA40940.1; -; mRNA
EMBL	X57115; CAA40398.2; -; mRNA
EMBL	D10480; BAA01283.1; -; Genomic_DNA
EMBL	M58440; AAA41914.1; -; mRNA
IPI	IPI00201410; -; .
IPI	IPI00559849; -; .
PIR	A33264; A33264; .
RefSeq	NP_058737.1; NM_017041.1; .
UniGene	Rn.6866; -; .
ProteinModelPortal	P63329; -; .
SMR	P63329; 1-372; .
STRING	P63329; -; .
PhosphoSite	P63329; -; .
PRIDE	P63329; -; .
GeneID	24674; -; .
KEGG	rno:24674; -; .
UCSC	NM_017041; rat; .
CTD	5530; -; .
RGD	3382; Ppp3ca; .
eggNOG	roNOG11256; -; .
GeneTree	ENSGT00530000063087; -; .
HOVERGEN	HBG002819; -; .
InParanoid	P63329; -; .
OrthoDB	EOG4PVNZK; -; .
PhylomeDB	P63329; -; .
NextBio	604055; -; .
ArrayExpress	P63329; -; .
Genevestigator	P63329; -; .
GermOnline	ENSRNOG00000009882; Rattus norvegicus; .
GO	GO:0005955; C:calcineurin complex; IDA:RGD; .
GO	GO:0005829; C:cytosol; IDA:RGD; .
GO	GO:0016020; C:membrane; IDA:RGD; .
GO	GO:0005634; C:nucleus; IEA:UniProtKB-SubCell; .
GO	GO:0005625; C:soluble fraction; IDA:RGD; .
GO	GO:0004723; F:calcium-dependent protein serine/threonine phosphatase activity; IDA:RGD; .
GO	GO:0005516; F:calmodulin binding; IDA:RGD; .
GO	GO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GO	GO:0046982; F:protein heterodimerization activity; IDA:RGD; .
GO	GO:0071333; P:cellular response to glucose stimulus; IMP:BHF-UCL; .
GO	GO:0045955; P:negative regulation of calcium ion-dependent exocytosis; IC:BHF-UCL; .
GO	GO:0046676; P:negative regulation of insulin secretion; IMP:BHF-UCL; .
GO	GO:0006470; P:protein dephosphorylation; IDA:RGD; .
GO	GO:0001975; P:response to amphetamine; IEP:RGD; .
InterPro	IPR004843; Metallo_PEstase_dom; .
InterPro	IPR006186; Ser/Thr-sp_prot-phosphatase; .
KO	K04348; -; .
Pfam	PF00149; Metallophos; 1; .
PRINTS	PR00114; STPHPHTASE; .
SMART	SM00156; PP2Ac; 1; .
PROSITE	PS00125; SER_THR_PHOSPHATASE; 1; .

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server

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