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World-2DPAGE Repository  Search by  [accession number]  [description, ID or gene]  [author names]  [spot ID / serial number]  [identification methods]  [pI / Mw range]  [combined fields]  Maps  [experimental info]  [protein list]  [graphical interface]      Databases 0001 0002 0003 0004 0005 0006 0007 0008 0009 0010 0011 0012 0014 0017 0018 0019 0020 0021 0022 0023 0024 0026 0027 0029 0030 0031 0032 0034 0035 0036 0037 0038 0039 0040 0042 0043 0044 0046 0047 0050 0051 0053 0054 0056 0059 0061 0062 0063 0066 0068 0069 0076 0077 0080 0084 0085 0088 use 'Ctrl' to select several databases Select Remote Interfaces [All Interfaces] SWISS-2DPAGE World-2DPAGE Portal Exclude local DBs has only effect if a remote interface is selected

Searching in 'World-2DPAGE Repository [0030]' for entry matching: PDIA1_RAT World-2DPAGE Repository (0030):  PDIA1_RAT PDIA1_RAT General information about the entry View entry in simple text format Entry name PDIA1_RAT Primary accession number P04785 integrated into World-2DPAGE Repository (0030) on  November 9, 2010 (release 1) 2D Annotations were last modified on June 21, 2011 (version 2) General Annotations were last modified on  November 23, 2011 (version 2) Name and origin of the protein Description RecName: Full=Protein disulfide-isomerase; Short=PDI; EC=5.3.4.1; AltName: Full=Cellular thyroid hormone-binding protein; AltName: Full=Prolyl 4-hydroxylase subunit beta; Flags: Precursor;. Gene name Name=P4hb Synonyms=Pdia1 Annotated species Rattus norvegicus (Rat) [TaxID: 10116] Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus. References [1]   2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000006; D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C. ''Two-dimensional gel proteome reference map of INS-1E cells'' Proteomics 11(7):1365-1369 (2011) 2D PAGE maps for identified proteins How to interpret a protein RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7} Rattus norvegicus (Rat)   map experimental info   RAT_INS1E_4-7 MAP LOCATIONS: SPOT 846: pI=5.64; Mw=67834   [identification data] SPOT 860: pI=4.71; Mw=66625   [identification data] SPOT 872: pI=4.74; Mw=66533   [identification data] IDENTIFICATION: SPOT 846: Mascot protein score=82. Number of peptides=3 [1] SPOT 860: Mascot protein score=283. Number of peptides=7 [1]; SPOT 872: Mascot protein score=484. Number of peptides=10 [1]. MAPPING (identification): SPOT 846: Tandem mass spectrometry [1]; SPOT 860: Tandem mass spectrometry [1]; SPOT 872: Tandem mass spectrometry [1]. Copyright Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium Cross-references UniProtKB/Swiss-Prot P04785; PDIA1_RAT. World-2DPAGE Repository P04785; PDIA1_RAT. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_11 Entry name PDIA1_RAT Primary accession number P04785 Secondary accession number(s) P13700 Sequence was last modified on  October 1, 1989 (version 2) Annotations were last modified on  November 16, 2011 (version 125) Name and origin of the protein Description RecName: Full=Protein disulfide-isomerase; Short=PDI; EC=5.3.4.1; AltName: Full=Cellular thyroid hormone-binding protein; AltName: Full=Prolyl 4-hydroxylase subunit beta; Flags: Precursor; Gene name Name=P4hb Synonyms=Pdia1 Encoded on Name=P4hb; Synonyms=Pdia1 Keywords Cell membrane; Chaperone; Complete proteome; Direct protein sequencing; Disulfide bond; Endoplasmic reticulum; Isomerase; Membrane; Redox-active center; Reference proteome; Repeat; Signal. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL M21018; AAA40620.1; -; mRNA EMBL BC061857; AAH61857.1; -; mRNA EMBL X02918; CAA26675.1; -; mRNA EMBL M21476; AAA40619.1; -; mRNA IPI IPI00198887; -; . PIR A24595; ISRTSS; . PIR S68028; S68028; . RefSeq NP_037130.1; NM_012998.1; . UniGene Rn.4234; -; . ProteinModelPortal P04785; -; . SMR P04785; 20-359; 370-473; . IntAct P04785; 1; . STRING P04785; -; . PhosphoSite P04785; -; . PRIDE P04785; -; . Ensembl ENSRNOT00000054958; ENSRNOP00000051841; ENSRNOG00000036689; . GeneID 25506; -; . KEGG rno:25506; -; . UCSC NM_012998; rat; . CTD 5034; -; . RGD 3244; P4hb; . eggNOG roNOG08939; -; . GeneTree ENSGT00590000082936; -; . HOVERGEN HBG005920; -; . InParanoid P04785; -; . OMA LMSQDLP; -; . OrthoDB EOG4JWVDB; -; . PhylomeDB P04785; -; . NextBio 606925; -; . Genevestigator P04785; -; . GermOnline ENSRNOG00000036689; Rattus norvegicus; . GO GO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell; . GO GO:0042470; C:melanosome; IEA:UniProtKB-SubCell; . GO GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell; . GO GO:0009055; F:electron carrier activity; IEA:InterPro; . GO GO:0003756; F:protein disulfide isomerase activity; IMP:RGD; . GO GO:0015035; F:protein disulfide oxidoreductase activity; IEA:InterPro; . GO GO:0046982; F:protein heterodimerization activity; IPI:RGD; . GO GO:0045454; P:cell redox homeostasis; IEA:InterPro; . GO GO:0006662; P:glycerol ether metabolic process; IEA:InterPro; . InterPro IPR005788; Disulphide_isomerase; . InterPro IPR005792; Prot_disulphide_isomerase; . InterPro IPR005746; Thioredoxin; . InterPro IPR012336; Thioredoxin-like_fold; . InterPro IPR017937; Thioredoxin_CS; . InterPro IPR013766; Thioredoxin_domain; . Gene3D G3DSA:3.40.30.10; Thioredoxin_fold; 3; . KO K09580; -; . Pfam PF00085; Thioredoxin; 2; . PRINTS PR00421; THIOREDOXIN; . SUPFAM SSF52833; Thiordxn-like_fd; 4; . TIGRFAMs TIGR01130; ER_PDI_fam; 1; . TIGRFAMs TIGR01126; Pdi_dom; 2; . PROSITE PS00014; ER_TARGET; 1; . PROSITE PS00194; THIOREDOXIN_1; 2; . PROSITE PS51352; THIOREDOXIN_2; 2; . World-2DPAGE Repository (search AC)