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Searching in 'World-2DPAGE Repository [0030]' for entry matching: SYG_RAT




World-2DPAGE Repository (0030):  SYG_RAT

SYG_RAT


General information about the entry
View entry in simple text format
Entry nameSYG_RAT
Primary accession numberQ5I0G4
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Glycyl-tRNA synthetase; EC=6.1.1.14; AltName: Full=Diadenosine tetraphosphate synthetase; Short=AP-4-A synthetase; AltName: Full=Glycine--tRNA ligase; Short=GlyRS; Flags: Fragment;.
Gene nameName=Gars
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info 		 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=6.06; Mw=82786  [identification data]
pI=6.04; Mw=81762  [identification data]

IDENTIFICATION: SPOT 479: Mascot protein score=363. Number of peptides=11 [1]
SPOT 508: Mascot protein score=496. Number of peptides=11 [1].
MAPPING (identification):
SPOT 479: Tandem mass spectrometry [1];
SPOT 508: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtQ5I0G4; SYG_RAT.
World-2DPAGE RepositoryQ5I0G4; SYG_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameSYG_RAT
Primary accession numberQ5I0G4
Sequence was last modified on February 15, 2005 (version 1)
Annotations were last modified on November 16, 2011 (version 61)
Name and origin of the protein
DescriptionRecName: Full=Glycyl-tRNA synthetase; EC=6.1.1.14; AltName: Full=Diadenosine tetraphosphate synthetase; Short=AP-4-A synthetase; AltName: Full=Glycine--tRNA ligase; Short=GlyRS; Flags: Fragment;
Gene nameName=Gars
Encoded onName=Gars
KeywordsAcetylation; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Mitochondrion; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLBC088347; AAH88347.1; -; mRNA
IPIIPI00364262; -; .
UniGeneRn.3338; -; .
HSSPP56206; 1ATI; .
ProteinModelPortalQ5I0G4; -; .
STRINGQ5I0G4; -; .
PhosphoSiteQ5I0G4; -; .
PRIDEQ5I0G4; -; .
NMPDRfig|10116.3.peg.21310; -; .
RGD1307856; Gars; .
eggNOGroNOG09456; -; .
GeneTreeENSGT00390000016949; -; .
HOVERGENHBG036190; -; .
InParanoidQ5I0G4; -; .
OrthoDBEOG4RV2QV; -; .
PhylomeDBQ5I0G4; -; .
ArrayExpressQ5I0G4; -; .
GenevestigatorQ5I0G4; -; .
GOGO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0004820; F:glycine-tRNA ligase activity; ISS:UniProtKB; .
GOGO:0046983; F:protein dimerization activity; ISS:UniProtKB; .
GOGO:0015966; P:diadenosine tetraphosphate biosynthetic process; ISS:UniProtKB; .
GOGO:0006426; P:glycyl-tRNA aminoacylation; IEA:InterPro; .
InterProIPR002314; aa-tRNA-synt_IIb_cons-dom; .
InterProIPR006195; aa-tRNA-synth_II; .
InterProIPR004154; Anticodon-bd; .
InterProIPR002315; tRNA-synt_gly; .
Gene3DG3DSA:3.40.50.800; Anticodon_bd; 1; .
PANTHERPTHR10745; tRNA-synt_gly; 1; .
PfamPF03129; HGTP_anticodon; 1; .
PfamPF00587; tRNA-synt_2b; 1; .
PRINTSPR01043; TRNASYNTHGLY; .
SUPFAMSSF52954; Anticodon_bd; 1; .
TIGRFAMsTIGR00389; GlyS_dimeric; 1; .
PROSITEPS50862; AA_TRNA_LIGASE_II; 1; .
PROSITEPS00762; WHEP_TRS_1; PARTIAL; .
PROSITEPS51185; WHEP_TRS_2; PARTIAL; .


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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server