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World-2DPAGE Repository  Search by  [accession number]  [description, ID or gene]  [author names]  [spot ID / serial number]  [identification methods]  [pI / Mw range]  [combined fields]  Maps  [experimental info]  [protein list]  [graphical interface]      Databases 0001 0002 0003 0004 0005 0006 0007 0008 0009 0010 0011 0012 0014 0017 0018 0019 0020 0021 0022 0023 0024 0026 0027 0029 0030 0031 0032 0034 0035 0036 0037 0038 0039 0040 0042 0043 0044 0046 0047 0050 0051 0053 0054 0056 0059 0061 0062 0063 0066 0068 0069 0076 0077 0080 0084 0085 0088 use 'Ctrl' to select several databases Select Remote Interfaces [All Interfaces] SWISS-2DPAGE World-2DPAGE Portal Exclude local DBs has only effect if a remote interface is selected

Searching in 'World-2DPAGE Repository [0030]' for entry matching: SYG_RAT World-2DPAGE Repository (0030):  SYG_RAT SYG_RAT General information about the entry View entry in simple text format Entry name SYG_RAT Primary accession number Q5I0G4 integrated into World-2DPAGE Repository (0030) on  November 9, 2010 (release 1) 2D Annotations were last modified on June 21, 2011 (version 2) General Annotations were last modified on  November 23, 2011 (version 2) Name and origin of the protein Description RecName: Full=Glycyl-tRNA synthetase; EC=6.1.1.14; AltName: Full=Diadenosine tetraphosphate synthetase; Short=AP-4-A synthetase; AltName: Full=Glycine--tRNA ligase; Short=GlyRS; Flags: Fragment;. Gene name Name=Gars Annotated species Rattus norvegicus (Rat) [TaxID: 10116] Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus. References [1]   2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000006; D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C. ''Two-dimensional gel proteome reference map of INS-1E cells'' Proteomics 11(7):1365-1369 (2011) 2D PAGE maps for identified proteins How to interpret a protein RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7} Rattus norvegicus (Rat)   map experimental info   RAT_INS1E_4-7 MAP LOCATIONS: SPOT 479: pI=6.06; Mw=82786   [identification data] SPOT 508: pI=6.04; Mw=81762   [identification data] IDENTIFICATION: SPOT 479: Mascot protein score=363. Number of peptides=11 [1] SPOT 508: Mascot protein score=496. Number of peptides=11 [1]. MAPPING (identification): SPOT 479: Tandem mass spectrometry [1]; SPOT 508: Tandem mass spectrometry [1]. Copyright Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium Cross-references UniProtKB/Swiss-Prot Q5I0G4; SYG_RAT. World-2DPAGE Repository Q5I0G4; SYG_RAT. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_11 Entry name SYG_RAT Primary accession number Q5I0G4 Sequence was last modified on  February 15, 2005 (version 1) Annotations were last modified on  November 16, 2011 (version 61) Name and origin of the protein Description RecName: Full=Glycyl-tRNA synthetase; EC=6.1.1.14; AltName: Full=Diadenosine tetraphosphate synthetase; Short=AP-4-A synthetase; AltName: Full=Glycine--tRNA ligase; Short=GlyRS; Flags: Fragment; Gene name Name=Gars Encoded on Name=Gars Keywords Acetylation; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Mitochondrion; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL BC088347; AAH88347.1; -; mRNA IPI IPI00364262; -; . UniGene Rn.3338; -; . HSSP P56206; 1ATI; . ProteinModelPortal Q5I0G4; -; . STRING Q5I0G4; -; . PhosphoSite Q5I0G4; -; . PRIDE Q5I0G4; -; . NMPDR fig|10116.3.peg.21310; -; . RGD 1307856; Gars; . eggNOG roNOG09456; -; . GeneTree ENSGT00390000016949; -; . HOVERGEN HBG036190; -; . InParanoid Q5I0G4; -; . OrthoDB EOG4RV2QV; -; . PhylomeDB Q5I0G4; -; . ArrayExpress Q5I0G4; -; . Genevestigator Q5I0G4; -; . GO GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell; . GO GO:0005524; F:ATP binding; IEA:UniProtKB-KW; . GO GO:0004820; F:glycine-tRNA ligase activity; ISS:UniProtKB; . GO GO:0046983; F:protein dimerization activity; ISS:UniProtKB; . GO GO:0015966; P:diadenosine tetraphosphate biosynthetic process; ISS:UniProtKB; . GO GO:0006426; P:glycyl-tRNA aminoacylation; IEA:InterPro; . InterPro IPR002314; aa-tRNA-synt_IIb_cons-dom; . InterPro IPR006195; aa-tRNA-synth_II; . InterPro IPR004154; Anticodon-bd; . InterPro IPR002315; tRNA-synt_gly; . Gene3D G3DSA:3.40.50.800; Anticodon_bd; 1; . PANTHER PTHR10745; tRNA-synt_gly; 1; . Pfam PF03129; HGTP_anticodon; 1; . Pfam PF00587; tRNA-synt_2b; 1; . PRINTS PR01043; TRNASYNTHGLY; . SUPFAM SSF52954; Anticodon_bd; 1; . TIGRFAMs TIGR00389; GlyS_dimeric; 1; . PROSITE PS50862; AA_TRNA_LIGASE_II; 1; . PROSITE PS00762; WHEP_TRS_1; PARTIAL; . PROSITE PS51185; WHEP_TRS_2; PARTIAL; . World-2DPAGE Repository (search AC)