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Searching in 'World-2DPAGE Repository [0019]' for entry matching: TPIS_RAT




World-2DPAGE Repository (0019):  TPIS_RAT


TPIS_RAT


General information about the entry
View entry in simple text format
Entry nameTPIS_RAT
Primary accession numberP48500
integrated into World-2DPAGE Repository (0019) on December 9, 2009 (release 1)
2D Annotations were last modified onMay 30, 2011 (version 2)
General Annotations were last modified on January 13, 2012 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Triosephosphate isomerase; Short=TIM; EC=5.3.1.1; AltName: Full=Triose-phosphate isomerase;.
Gene nameName=Tpi1
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1111/j.1471-4159.2010.06719.x;
VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M.
''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome''
J of Neurochemistry 113(6):1577-1588 (2010)
2D PAGE maps for identified proteins
How to interpret a protein

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus
HIPP_SYNAP
  map experimental info
 
HIPP_SYNAP

MAP LOCATIONS:
pI=7.07; Mw=27213  [identification data]
pI=6.45; Mw=27417  [identification data]
pI=7.07; Mw=27213  [identification data]
pI=6.45; Mw=27417  [identification data]
pI=6.45; Mw=27417  [identification data]
pI=6.45; Mw=27417  [identification data]

IDENTIFICATION: SPOT 767: Peptides number=2. SeqCov=8%. MOWSE score=2.09E+2 [1]
SPOT 768: Peptides number=7. SeqCov=45%. MOWSE score=1.26E+9 [1]; SPOT 770: Peptides number=4. SeqCov=20.9%. MOWSE score=2.99E+4 [1]; SPOT 771: Peptides number=7. SeqCov=35.7%. MOWSE score=4.28E+5 [1]; SPOT 782: Peptides number=7. SeqCov=38.6%. MOWSE score=1.76E+7 [1]; SPOT 1038: Peptides number=3. SeqCov=17.3%. MOWSE score=3.96E+2 [1].
MAPPING (identification):
SPOT 767: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 768: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 770: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 771: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 782: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 1038: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Willard Freeman, Penn State College of Medicine, USA
Cross-references
UniProtKB/Swiss-ProtP48500; TPIS_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry nameTPIS_RAT
Primary accession numberP48500
Secondary accession number(s) A0JN18 Q6P793
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on November 16, 2011 (version 86)
Name and origin of the protein
DescriptionRecName: Full=Triosephosphate isomerase; Short=TIM; EC=5.3.1.1; AltName: Full=Triose-phosphate isomerase;
Gene nameName=Tpi1
Encoded onName=Tpi1
KeywordsAcetylation; Complete proteome; Direct protein sequencing; Gluconeogenesis; Glycolysis; Isomerase; Pentose shunt; Phosphoprotein; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLL36250; AAA42278.1; -; mRNA
EMBLBC061781; AAH61781.1; -; mRNA
EMBLBC126087; AAI26088.1; -; mRNA
IPIIPI00231767; -; .
RefSeqNP_075211.2; NM_022922.2; .
UniGeneRn.144555; -; .
UniGeneRn.37838; -; .
ProteinModelPortalP48500; -; .
SMRP48500; 3-249; .
IntActP48500; 2; .
STRINGP48500; -; .
PhosphoSiteP48500; -; .
Rat-heart-2DPAGEP48500; -; .
World-2DPAGE0004:P48500; -; .
PRIDEP48500; -; .
EnsemblENSRNOT00000020647; ENSRNOP00000020647; ENSRNOG00000015290; .
GeneID24849; -; .
KEGGrno:24849; -; .
NMPDRfig|10116.3.peg.22207; -; .
UCSCNM_022922; rat; .
CTD7167; -; .
RGD3896; Tpi1; .
eggNOGroNOG08237; -; .
HOVERGENHBG002599; -; .
InParanoidP48500; -; .
OMACPAIYLM; -; .
OrthoDBEOG40S0GF; -; .
PhylomeDBP48500; -; .
NextBio604616; -; .
ArrayExpressP48500; -; .
GenevestigatorP48500; -; .
GermOnlineENSRNOG00000015290; Rattus norvegicus; .
GOGO:0005829; C:cytosol; IDA:RGD; .
GOGO:0005634; C:nucleus; IDA:RGD; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0005515; F:protein binding; IPI:RGD; .
GOGO:0004807; F:triose-phosphate isomerase activity; IDA:RGD; .
GOGO:0006094; P:gluconeogenesis; IEA:UniProtKB-KW; .
GOGO:0006096; P:glycolysis; TAS:RGD; .
GOGO:0006098; P:pentose-phosphate shunt; IEA:UniProtKB-KW; .
InterProIPR013785; Aldolase_TIM; .
InterProIPR022896; TrioseP_Isoase_bac/euk; .
InterProIPR000652; Triosephosphate_isomerase; .
InterProIPR020861; Triosephosphate_isomerase_AS; .
Gene3DG3DSA:3.20.20.70; Aldolase_TIM; 1; .
KOK01803; -; .
PANTHERPTHR21139; Triophos_ismrse; 1; .
PfamPF00121; TIM; 1; .
SUPFAMSSF51351; Triophos_ismrse; 1; .
TIGRFAMsTIGR00419; Tim; 1; .
PROSITEPS00171; TIM_1; 1; .
PROSITEPS51440; TIM_2; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server