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World-2DPAGE Repository  Search by  [accession number]  [description, ID or gene]  [author names]  [spot ID / serial number]  [identification methods]  [pI / Mw range]  [combined fields]  Maps  [experimental info]  [protein list]  [graphical interface]      Databases 0001 0002 0003 0004 0005 0006 0007 0008 0009 0010 0011 0012 0014 0017 0018 0019 0020 0021 0022 0023 0024 0026 0027 0029 0030 0031 0032 0034 0035 0036 0037 0038 0039 0040 0042 0043 0044 0046 0047 0050 0051 0053 0054 0056 0059 0061 0062 0063 0066 0068 0069 0076 0077 0080 0084 0085 0088 use 'Ctrl' to select several databases Select Remote Interfaces [All Interfaces] SWISS-2DPAGE World-2DPAGE Portal Exclude local DBs has only effect if a remote interface is selected

Searching in 'World-2DPAGE Repository [0030]' for entry matching: UBE2K_MOUSE World-2DPAGE Repository (0030):  UBE2K_MOUSE UBE2K_MOUSE General information about the entry View entry in simple text format Entry name UBE2K_MOUSE Primary accession number P61087 integrated into World-2DPAGE Repository (0030) on  November 9, 2010 (release 1) 2D Annotations were last modified on June 21, 2011 (version 2) General Annotations were last modified on  November 23, 2011 (version 2) Name and origin of the protein Description RecName: Full=Ubiquitin-conjugating enzyme E2 K; EC=6.3.2.19; AltName: Full=Huntingtin-interacting protein 2; Short=HIP-2; AltName: Full=Ubiquitin carrier protein; AltName: Full=Ubiquitin-conjugating enzyme E2-25 kDa; Short=Ubiquitin-conjugating enzyme E2(25K); Short=Ubiquitin-conjugating enzyme E2-25K; AltName: Full=Ubiquitin-protein ligase;. Gene name Name=Ube2k Synonyms=Hip2 Annotated species Rattus norvegicus (Rat) [TaxID: 10116] Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus. References [1]   2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000006; D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C. ''Two-dimensional gel proteome reference map of INS-1E cells'' Proteomics 11(7):1365-1369 (2011) 2D PAGE maps for identified proteins How to interpret a protein RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7} Rattus norvegicus (Rat)   map experimental info   RAT_INS1E_4-7 MAP LOCATIONS: SPOT 2244: pI=5.19; Mw=25614   [identification data] SPOT 2266: pI=5.17; Mw=25054   [identification data] IDENTIFICATION: SPOT 2244: Mascot protein score=247. Number of peptides=5 [1] SPOT 2266: Mascot protein score=61. Number of peptides=1 [1]. MAPPING (identification): SPOT 2244: Tandem mass spectrometry [1]; SPOT 2266: Tandem mass spectrometry [1]. Copyright Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium Cross-references REPRODUCTION-2DPAGE P61087; P61087. UniProtKB/Swiss-Prot P61087; UBE2K_MOUSE. World-2DPAGE Repository P61087; UBE2K_MOUSE. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_11 Entry name UBE2K_MOUSE Primary accession number P61087 Secondary accession number(s) O54806 P27924 Q16721 Q9CVV9 Sequence was last modified on  January 23, 2007 (version 3) Annotations were last modified on  November 16, 2011 (version 87) Name and origin of the protein Description RecName: Full=Ubiquitin-conjugating enzyme E2 K; EC=6.3.2.19; AltName: Full=Huntingtin-interacting protein 2; Short=HIP-2; AltName: Full=Ubiquitin carrier protein; AltName: Full=Ubiquitin-conjugating enzyme E2-25 kDa; Short=Ubiquitin-conjugating enzyme E2(25K); Short=Ubiquitin-conjugating enzyme E2-25K; AltName: Full=Ubiquitin-protein ligase; Gene name Name=Ube2k Synonyms=Hip2 Encoded on Name=Ube2k; Synonyms=Hip2 Keywords Acetylation; ATP-binding; Complete proteome; Cytoplasm; Isopeptide bond; Ligase; Nucleotide-binding; Reference proteome; Ubl conjugation; Ubl conjugation pathway. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL AB011081; BAA24927.1; -; mRNA EMBL BC002013; AAH02013.1; -; mRNA EMBL BC085311; AAH85311.1; -; mRNA EMBL AK006316; BAB24523.1; -; mRNA IPI IPI00322440; -; . RefSeq NP_058066.2; NM_016786.3; . UniGene Mm.319512; -; . UniGene Mm.441014; -; . ProteinModelPortal P61087; -; . SMR P61087; 3-200; . STRING P61087; -; . PhosphoSite P61087; -; . REPRODUCTION-2DPAGE P61087; -; . PRIDE P61087; -; . Ensembl ENSMUST00000142407; ENSMUSP00000122471; ENSMUSG00000029203; . GeneID 53323; -; . KEGG mmu:53323; -; . CTD 3093; -; . MGI MGI:1858216; Ube2k; . GeneTree ENSGT00590000083155; -; . HOGENOM HBG756483; -; . HOVERGEN HBG063308; -; . InParanoid P61087; -; . OMA MAVSRIK; -; . PhylomeDB P61087; -; . NextBio 310133; -; . ArrayExpress P61087; -; . Bgee P61087; -; . Genevestigator P61087; -; . GermOnline ENSMUSG00000029203; Mus musculus; . GO GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; . GO GO:0005524; F:ATP binding; IEA:UniProtKB-KW; . GO GO:0031625; F:ubiquitin protein ligase binding; ISS:UniProtKB; . GO GO:0070936; P:protein K48-linked ubiquitination; ISS:UniProtKB; . InterPro IPR009060; UBA-like; . InterPro IPR000449; UBA/transl_elong_EF1B_N; . InterPro IPR015940; UBA/transl_elong_EF1B_N_euk; . InterPro IPR000608; UBQ-conjugat_E2; . InterPro IPR023313; UBQ-conjugating_AS; . InterPro IPR016135; UBQ-conjugating_enzyme/RWD; . Gene3D G3DSA:3.10.110.10; UBQ-conjugat_E2; 1; . KO K04649; -; . Pfam PF00627; UBA; 1; . Pfam PF00179; UQ_con; 1; . SMART SM00165; UBA; 1; . SUPFAM SSF46934; UBA_like; 1; . SUPFAM SSF54495; UBQ-conjugat/RWD-like; 1; . PROSITE PS50030; UBA; 1; . PROSITE PS00183; UBIQUITIN_CONJUGAT_1; 1; . PROSITE PS50127; UBIQUITIN_CONJUGAT_2; 1; . World-2DPAGE Repository (search AC)