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Searching in 'World-2DPAGE Repository [0030]' for entry matching: UBE2K_MOUSE




World-2DPAGE Repository (0030):  UBE2K_MOUSE

UBE2K_MOUSE


General information about the entry
View entry in simple text format
Entry nameUBE2K_MOUSE
Primary accession numberP61087
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Ubiquitin-conjugating enzyme E2 K; EC=6.3.2.19; AltName: Full=Huntingtin-interacting protein 2; Short=HIP-2; AltName: Full=Ubiquitin carrier protein; AltName: Full=Ubiquitin-conjugating enzyme E2-25 kDa; Short=Ubiquitin-conjugating enzyme E2(25K); Short=Ubiquitin-conjugating enzyme E2-25K; AltName: Full=Ubiquitin-protein ligase;.
Gene nameName=Ube2k
Synonyms=Hip2
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info 		 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=5.19; Mw=25614  [identification data]
pI=5.17; Mw=25054  [identification data]

IDENTIFICATION: SPOT 2244: Mascot protein score=247. Number of peptides=5 [1]
SPOT 2266: Mascot protein score=61. Number of peptides=1 [1].
MAPPING (identification):
SPOT 2244: Tandem mass spectrometry [1];
SPOT 2266: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
REPRODUCTION-2DPAGEP61087; P61087.
UniProtKB/Swiss-ProtP61087; UBE2K_MOUSE.
World-2DPAGE RepositoryP61087; UBE2K_MOUSE.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameUBE2K_MOUSE
Primary accession numberP61087
Secondary accession number(s) O54806 P27924 Q16721 Q9CVV9
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on November 16, 2011 (version 87)
Name and origin of the protein
DescriptionRecName: Full=Ubiquitin-conjugating enzyme E2 K; EC=6.3.2.19; AltName: Full=Huntingtin-interacting protein 2; Short=HIP-2; AltName: Full=Ubiquitin carrier protein; AltName: Full=Ubiquitin-conjugating enzyme E2-25 kDa; Short=Ubiquitin-conjugating enzyme E2(25K); Short=Ubiquitin-conjugating enzyme E2-25K; AltName: Full=Ubiquitin-protein ligase;
Gene nameName=Ube2k
Synonyms=Hip2
Encoded onName=Ube2k; Synonyms=Hip2
KeywordsAcetylation; ATP-binding; Complete proteome; Cytoplasm; Isopeptide bond; Ligase; Nucleotide-binding; Reference proteome; Ubl conjugation; Ubl conjugation pathway.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAB011081; BAA24927.1; -; mRNA
EMBLBC002013; AAH02013.1; -; mRNA
EMBLBC085311; AAH85311.1; -; mRNA
EMBLAK006316; BAB24523.1; -; mRNA
IPIIPI00322440; -; .
RefSeqNP_058066.2; NM_016786.3; .
UniGeneMm.319512; -; .
UniGeneMm.441014; -; .
ProteinModelPortalP61087; -; .
SMRP61087; 3-200; .
STRINGP61087; -; .
PhosphoSiteP61087; -; .
REPRODUCTION-2DPAGEP61087; -; .
PRIDEP61087; -; .
EnsemblENSMUST00000142407; ENSMUSP00000122471; ENSMUSG00000029203; .
GeneID53323; -; .
KEGGmmu:53323; -; .
CTD3093; -; .
MGIMGI:1858216; Ube2k; .
GeneTreeENSGT00590000083155; -; .
HOGENOMHBG756483; -; .
HOVERGENHBG063308; -; .
InParanoidP61087; -; .
OMAMAVSRIK; -; .
PhylomeDBP61087; -; .
NextBio310133; -; .
ArrayExpressP61087; -; .
BgeeP61087; -; .
GenevestigatorP61087; -; .
GermOnlineENSMUSG00000029203; Mus musculus; .
GOGO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0031625; F:ubiquitin protein ligase binding; ISS:UniProtKB; .
GOGO:0070936; P:protein K48-linked ubiquitination; ISS:UniProtKB; .
InterProIPR009060; UBA-like; .
InterProIPR000449; UBA/transl_elong_EF1B_N; .
InterProIPR015940; UBA/transl_elong_EF1B_N_euk; .
InterProIPR000608; UBQ-conjugat_E2; .
InterProIPR023313; UBQ-conjugating_AS; .
InterProIPR016135; UBQ-conjugating_enzyme/RWD; .
Gene3DG3DSA:3.10.110.10; UBQ-conjugat_E2; 1; .
KOK04649; -; .
PfamPF00627; UBA; 1; .
PfamPF00179; UQ_con; 1; .
SMARTSM00165; UBA; 1; .
SUPFAMSSF46934; UBA_like; 1; .
SUPFAMSSF54495; UBQ-conjugat/RWD-like; 1; .
PROSITEPS50030; UBA; 1; .
PROSITEPS00183; UBIQUITIN_CONJUGAT_1; 1; .
PROSITEPS50127; UBIQUITIN_CONJUGAT_2; 1; .


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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server