World-2DPAGE Home
Home  |  Contact
Due to scheduled maintenance work, this service will not be available from Tuesday August 23rd 06:00 pm until Wednesday August 24th 08:00 am CEST. Apologies for the inconvenience.

World-2DPAGE Repository 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 
Maps  [experimental info] 
[protein list] 
[graphical interface]
     Databases

use 'Ctrl' to select several
databases

Select Remote Interfaces
[All Interfaces]
SWISS-2DPAGE
World-2DPAGE Portal
Exclude local DBs
has only effect if a remote
interface is selected
     
Searching in 'World-2DPAGE Repository [0019]' for entry matching: VATB2_RAT




World-2DPAGE Repository (0019):  VATB2_RAT

VATB2_RAT


General information about the entry
View entry in simple text format
Entry nameVATB2_RAT
Primary accession numberP62815
integrated into World-2DPAGE Repository (0019) on December 9, 2009 (release 1)
2D Annotations were last modified onMay 30, 2011 (version 2)
General Annotations were last modified on January 13, 2012 (version 2)
Name and origin of the protein
DescriptionRecName: Full=V-type proton ATPase subunit B, brain isoform; Short=V-ATPase subunit B 2; AltName: Full=Endomembrane proton pump 58 kDa subunit; AltName: Full=Vacuolar proton pump subunit B 2;.
Gene nameName=Atp6v1b2
Synonyms=Atp6b2, Vat2
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1111/j.1471-4159.2010.06719.x;
VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M.
''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome''
J of Neurochemistry 113(6):1577-1588 (2010)
2D PAGE maps for identified proteins
How to interpret a protein

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus
HIPP_SYNAP
  map experimental info 		 
HIPP_SYNAP

MAP LOCATIONS:
pI=5.57; Mw=56857  [identification data]
pI=5.57; Mw=56857  [identification data]
pI=5.57; Mw=56857  [identification data]

IDENTIFICATION: SPOT 413: Peptides number=15. SeqCov=36.8%. MOWSE score=2.10E+8 [1]
SPOT 1155: Peptides number=14. SeqCov=33.7%. MOWSE score=5.91E+9 [1]; SPOT 1160: Peptides number=14. SeqCov=35.6%. MOWSE score=5.31E+10 [1].
MAPPING (identification):
SPOT 413: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 1155: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 1160: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Willard Freeman, Penn State College of Medicine, USA
Cross-references
UniProtKB/Swiss-ProtP62815; VATB2_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry nameVATB2_RAT
Primary accession numberP62815
Secondary accession number(s) O09045 P50517
Sequence was last modified on August 16, 2004 (version 1)
Annotations were last modified on November 16, 2011 (version 70)
Name and origin of the protein
DescriptionRecName: Full=V-type proton ATPase subunit B, brain isoform; Short=V-ATPase subunit B 2; AltName: Full=Endomembrane proton pump 58 kDa subunit; AltName: Full=Vacuolar proton pump subunit B 2;
Gene nameName=Atp6v1b2
Synonyms=Atp6b2, Vat2
Encoded onName=Atp6v1b2; Synonyms=Atp6b2, Vat2
KeywordsComplete proteome; Direct protein sequencing; Hydrogen ion transport; Hydrolase; Ion transport; Membrane; Phosphoprotein; Reference proteome; Transport.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLY12635; CAA73183.1; -; mRNA
EMBLBC085714; AAH85714.1; -; mRNA
IPIIPI00199305; -; .
RefSeqNP_476561.1; NM_057213.2; .
UniGeneRn.8109; -; .
ProteinModelPortalP62815; -; .
SMRP62815; 53-507; .
IntActP62815; 1; .
STRINGP62815; -; .
PhosphoSiteP62815; -; .
World-2DPAGE0004:P62815; -; .
PRIDEP62815; -; .
EnsemblENSRNOT00000015931; ENSRNOP00000015931; ENSRNOG00000011891; .
GeneID117596; -; .
KEGGrno:117596; -; .
UCSCNM_057213; rat; .
CTD526; -; .
RGD620284; Atp6v1b2; .
eggNOGroNOG17349; -; .
GeneTreeENSGT00550000074724; -; .
HOVERGENHBG002176; -; .
InParanoidP62815; -; .
OrthoDBEOG4HMJ93; -; .
PhylomeDBP62815; -; .
NextBio620435; -; .
ArrayExpressP62815; -; .
GenevestigatorP62815; -; .
GermOnlineENSRNOG00000011891; Rattus norvegicus; .
GOGO:0005829; C:cytosol; ISS:UniProtKB; .
GOGO:0016021; C:integral to membrane; IDA:RGD; .
GOGO:0042470; C:melanosome; IEA:UniProtKB-SubCell; .
GOGO:0005886; C:plasma membrane; ISS:UniProtKB; .
GOGO:0033180; C:proton-transporting V-type ATPase; V1 domain; IEA:InterPro
GOGO:0005773; C:vacuole; TAS:GOC; .
GOGO:0005524; F:ATP binding; IEA:InterPro; .
GOGO:0046933; F:hydrogen ion transporting ATP synthase activity; rotational mechanism; IEA:InterPro
GOGO:0046961; F:proton-transporting ATPase activity; rotational mechanism; IEA:InterPro
GOGO:0015991; P:ATP hydrolysis coupled proton transport; IEA:InterPro; .
GOGO:0007035; P:vacuolar acidification; TAS:RGD; .
InterProIPR020003; ATPase_a/bsu_AS; .
InterProIPR000793; ATPase_F1/V1/A1-cplx_a/bsu_C; .
InterProIPR004100; ATPase_F1/V1/A1-cplx_a/bsu_N; .
InterProIPR000194; ATPase_F1/V1/A1_a/bsu_nucl-bd; .
InterProIPR005723; ATPase_V1-cplx_bsu; .
InterProIPR022879; V-ATPase_su_B/beta; .
KOK02147; -; .
PfamPF00006; ATP-synt_ab; 1; .
PfamPF00306; ATP-synt_ab_C; 1; .
PfamPF02874; ATP-synt_ab_N; 1; .
TIGRFAMsTIGR01040; V-ATPase_V1_B; 1; .
PROSITEPS00152; ATPASE_ALPHA_BETA; 1; .


World-2DPAGE Repository image

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server