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Searching in 'World-2DPAGE Repository [0002]' for entry matching: Q99W72

World-2DPAGE Repository (0002):  Q99W72


General information about the entry
View entry in simple text format
Primary accession numberQ99W72
integrated into World-2DPAGE Repository (0002) on December 7, 2005 (release 1)
2D Annotations were last modified onMay 1, 2011 (version 2)
General Annotations were last modified on November 14, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Putative TrmH family tRNA/rRNA methyltransferase; EC=2.1.1.-;.
Gene nameOrderedLocusNames=SAV0531
Annotated speciesStaphylococcus aureus (strain Mu50 / ATCC 700699) [TaxID: 158878]
TaxonomyBacteria; Firmicutes; Bacillales; Staphylococcus.
PubMed=17370256; DOI=10.1002/pmic.200600925; [NCBI, EBI, Israel, Japan]
Plikat U., Voshol H., Dangendorf Y., Wiedmann B., Devay P., Mueller D., Wirth U., Szustakowski J., Chirn G.W., Inverardi B., Puyang X., Brown K., Kamp H., Hoving S., Ruchti A., Brendlen N., Peterson R., Buco J., Oostrum J., Peitsch M.C.
''From proteomics to systems biology of bacterial pathogens: approaches, tools, and applications''
Proteomics 7(6):992-1003 (2007)
2D PAGE maps for identified proteins
How to interpret a protein

STAPHY4-7 {Staphylococcus aureus, pH 4-7}
Staphylococcus aureus (strain Mu50 / ATCC 700699)
  map experimental info

pI=6.61; Mw=28000  [identification data]

AREA: SPOT 2088: 1170 [1].
PEAK HEIGHT: SPOT 2088: 11346.262 [1].
STAPH_LSI: SPOT 2088: stapha_lsi_0531 [1].
MAPPING (identification):
SPOT 2088: Peptide mass fingerprinting [1].

STAPHY6-11 {Staphylococcus aureus, pH 6-11}
Staphylococcus aureus (strain Mu50 / ATCC 700699)
  map experimental info

pI=6.71; Mw=25000  [identification data]

AREA: SPOT 361: 1934 [1].
PEAK HEIGHT: SPOT 361: 8615 [1].
STAPHA_LSI: SPOT 361: stapha_lsi_1255 [1].
MAPPING (identification):
SPOT 361: Peptide mass fingerprinting [1].

Data from Dr. Hans Voshol, Novartis Institutes for BioMedical Research, Basel, Switzerland
UniProtKB/Swiss-ProtQ99W72; TRMHL_STAAM.
World-2DPAGE RepositoryQ99W72; TRMHL_STAAM.

2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Primary accession numberQ99W72
Sequence was last modified on June 1, 2001 (version 1)
Annotations were last modified on October 19, 2011 (version 52)
Name and origin of the protein
DescriptionRecName: Full=Putative TrmH family tRNA/rRNA methyltransferase; EC=2.1.1.-;
Gene nameOrderedLocusNames=SAV0531
Encoded onOrderedLocusNames=SAV0531
KeywordsComplete proteome; Methyltransferase; Transferase.
Copyrighted by the UniProt Consortium, see Distributed under the Creative Commons Attribution-NoDerivs License
EMBLBA000017; BAB56693.1; -; Genomic_DNA
RefSeqNP_371055.1; NC_002758.2; .
ProteinModelPortalQ99W72; -; .
STRINGQ99W72; -; .
World-2DPAGE0002:Q99W72; -; .
EnsemblBacteriaEBSTAT00000007259; EBSTAP00000007077; EBSTAG00000007258; .
GeneID1120504; -; .
GenomeReviewsBA000017_GR; SAV0531; .
KEGGsav:SAV0531; -; .
eggNOGCOG0566; -; .
GeneTreeEBGT00050000024953; -; .
HOGENOMHBG714057; -; .
ProtClustDBCLSK884713; -; .
BioCycSAUR158878:SAV0531-MONOMER; -; .
GOGO:0003723; F:RNA binding; IEA:InterPro; .
GOGO:0008173; F:RNA methyltransferase activity; IEA:InterPro; .
GOGO:0006396; P:RNA processing; IEA:InterPro; .
InterProIPR004441; rRNA_MeTrfase_3; .
InterProIPR001537; SpoU_MeTrfase; .
InterProIPR013123; SpoU_subst-bd; .
PfamPF00588; SpoU_methylase; 1; .
PfamPF08032; SpoU_sub_bind; 1; .
SMARTSM00967; SpoU_sub_bind; 1; .
TIGRFAMsTIGR00186; RRNA_methyl_3; 1; .

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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server