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Searching in 'World-2DPAGE Repository [0008]' for entry matching: Q59638




World-2DPAGE Repository (0008):  Q59638


Q59638


General information about the entry
View entry in simple text format
Entry nameODP2_PSEAE
Primary accession numberQ59638
integrated into World-2DPAGE Repository (0008) on February 5, 2009 (release 1)
2D Annotations were last modified onMay 27, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex; EC=2.3.1.12; AltName: Full=Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; AltName: Full=E2;.
Gene nameName=aceF
Synonyms=aceB
OrderedLocusNames=PA5016
Annotated speciesPseudomonas putida group [TaxID: 136845]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.200900142;
Cheng Z., Woody O.Z., Song J., Glick B.R., McConkey B.J.
''Proteome reference map for the plant growth-promotiong bacterium Pseudomonas putida UW4''
Proteomics 9(17):4271-4274 (2009)
2D PAGE maps for identified proteins
How to interpret a protein

PSEPU_UW4 {Pseudomonas putida UW4 proteome reference map}
Pseudomonas putida group
PSEPU_UW4
  map experimental info
 
PSEPU_UW4

MAP LOCATIONS:
pI=5.26; Mw=72600  [identification data]
pI=5.40; Mw=77000  [identification data]

IDENTIFICATION COMMENT: SPOT 15: SeqCov=4.2%. Mascot Protein score=79.65 [1]
SPOT 16: SeqCov=4.2%. Mascot Protein score=79.65 [1].
MAPPING (identification):
SPOT 15: Tandem mass spectrometry [1];
SPOT 16: Tandem mass spectrometry [1].

Copyright
Data from Dr. Zhenyu Cheng, University of Waterloo, Canada
Cross-references
UniProtKB/Swiss-ProtQ59638; ODP2_PSEAE.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameODP2_PSEAE
Primary accession numberQ59638
Sequence was last modified on December 8, 2000 (version 2)
Annotations were last modified on November 16, 2011 (version 87)
Name and origin of the protein
DescriptionRecName: Full=Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex; EC=2.3.1.12; AltName: Full=Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; AltName: Full=E2;
Gene nameName=aceF
Synonyms=aceB
OrderedLocusNames=PA5016
Encoded onName=aceF; Synonyms=aceB; OrderedLocusNames=PA5016
KeywordsAcyltransferase; Complete proteome; Glycolysis; Lipoyl; Reference proteome; Repeat; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLU47920; AAC45354.1; -; Genomic_DNA
EMBLAE004091; AAG08401.1; -; Genomic_DNA
PIRH83018; H83018; .
RefSeqNP_253703.1; NC_002516.2; .
ProteinModelPortalQ59638; -; .
SMRQ59638; 2-81; 305-547; .
World-2DPAGE0008:Q59638; -; .
PRIDEQ59638; -; .
GeneID881297; -; .
GenomeReviewsAE004091_GR; PA5016; .
KEGGpae:PA5016; -; .
NMPDRfig|208964.1.peg.5013; -; .
PseudoCAPPA5016; -; .
HOGENOMHBG630916; -; .
OMAMEIPAPK; -; .
ProtClustDBPRK11855; -; .
BioCycPAER208964:PA5016-MONOMER; -; .
GOGO:0045254; C:pyruvate dehydrogenase complex; IEA:InterPro; .
GOGO:0004742; F:dihydrolipoyllysine-residue acetyltransferase activity; IEA:EC; .
GOGO:0006096; P:glycolysis; IEA:UniProtKB-KW; .
InterProIPR003016; 2-oxoA_DH_lipoyl-BS; .
InterProIPR001078; 2-oxoacid_DH_actylTfrase; .
InterProIPR006256; AcTrfase_Pyrv_DH_cplx; .
InterProIPR000089; Biotin_lipoyl; .
InterProIPR023213; CAT-like_dom; .
InterProIPR004167; E3-bd; .
InterProIPR011053; Single_hybrid_motif; .
Gene3DG3DSA:3.30.559.10; CAT-like_dom; 1; .
Gene3DG3DSA:4.10.320.10; E3_bd; 1; .
KOK00627; -; .
PfamPF00198; 2-oxoacid_dh; 1; .
PfamPF00364; Biotin_lipoyl; 2; .
PfamPF02817; E3_binding; 1; .
SUPFAMSSF47005; E3_bd; 1; .
SUPFAMSSF51230; Hybrid_motif; 2; .
TIGRFAMsTIGR01348; PDHac_trf_long; 1; .
PROSITEPS50968; BIOTINYL_LIPOYL; 2; .
PROSITEPS00189; LIPOYL; 2; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server