Attention: World-2DPAGE is no longer maintained. Previously submitted data can still be queried at World-2DPAGE Repository.

Sample Preparation and Post-separation Analysis

3HIDH_HUMAN:

ID   3HIDH_HUMAN; PRELIMINARY; 2DG.
AC   P31937;
DT   01-AUG-1993, integrated into SWISS-2DPAGE (release 0).
DT   31-MAR-2004, 2D annotation version 2.
DT   19-MAY-2011, general annotation version 11.
DE   RecName: Full=3-hydroxyisobutyrate dehydrogenase, mitochondrial;
DE   Short=HIBADH; EC=1.1.1.31; Flags: Precursor;.
GN   Name=HIBADH;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   LIVER_HUMAN, LYMPHOCYTE_HUMAN, U937_HUMAN.
IM   LIVER_HUMAN, LYMPHOCYTE_HUMAN, U937_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=94147969; PubMed=8313870; [NCBI, Expasy, EBI, Israel, Japan]
RA   Hughes G.J., Frutiger S., Paquet N., Pasquali C., Sanchez J.-C., Tissot
RA   J.-D., Bairoch A., Appel R.D., Hochstrasser D.F.;
RT   ''''''Human liver protein map: update 1993'';'';'';
RL   Electrophoresis 14(1):1216-1222(1993).
RN   [2]
RP   MAPPING ON GEL.
RX   MEDLINE=96007934; PubMed=7498157; [NCBI, Expasy, EBI, Israel, Japan]
RA   Sanchez J.-C., Appel R.D., Golaz O.G., Pasquali C., Ravier F., Bairoch
RA   A., Hochstrasser D.F.;
RT   ''''''Inside SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 16(1):1131-1151(1995).
RN   [3]
RP   MAPPING ON GEL.
RA   Vuadens F., Crettaz D., Telenti A., Quadroni M., Duchosal M.A., Schneider
RA   P., Tissot J.-D.;
RT   ''''''New insights into HIV lymphocyte infection'';'';'';
RL   (IN) Sanchez J.-C., Corthals G.L., Hochstrasser D.F. (eds.);
RL   Biomedical Application of Proteomics, pp.245-262, Wiley-VCH, Weinheim (2004).
CC   -!- SUBUNIT: HOMODIMER 
2D   -!- MASTER: LIVER_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000184=5.53/29377;
2D   -!-   MAPPING: MICROSEQUENCING (ASKTPVGFIGL) [1].
2D   -!- MASTER: LYMPHOCYTE_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001YC6=5.52/27491;
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001YC6:
2D         (K)TPVGFIGLGNMoxGNPMoxAK(N),40-56; (K)EFQDAGEQVVSSPADVAEK(A),77-95;
2D         (K)MGAVFMDAPVSGGVGAAR(S),150-167; (K)MoxGAVFMoxDAPVSGGVGAAR(S),150-167; (K)DLGLAQDSATSTK(S),285-297.
2D   -!-   MAPPING: ESI tandem mass spectrometry [3].
2D   -!- MASTER: U937_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000D2Z=5.51/29570;
2D   -!-   MAPPING: MATCHING WITH THE LIVER MASTER GEL [2].
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DR   Siena-2DPAGE; P31937; D3HI_HUMAN.
DR   UniProtKB/Swiss-Prot; P31937; 3HIDH_HUMAN.
//

The following 2-D maps are available for this protein:

	Liver	Lymphocytes	Macrophage Like Cell Line

SWISS-2DPAGE (search AC)