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SWISS-2DPAGE 
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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: DLDH_ECOLI




SWISS-2DPAGE: DLDH_ECOLI



DLDH_ECOLI:

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ID   DLDH_ECOLI; STANDARD; 2DG.
AC   P0A9P0; P00391;
DT   01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT   31-MAR-2004, 2D annotation version 2.
DT   19-MAY-2011, general annotation version 14.
DE   RecName: Full=Dihydrolipoyl dehydrogenase; EC=1.8.1.4; AltName:
DE   Full=Dihydrolipoamide dehydrogenase; AltName: Full=E3 component of
DE   pyruvate and 2-oxoglutarate dehydrogenases complexes; AltName:
DE   Full=Glycine cleavage system L protein;.
GN   Name=lpdA; Synonyms=lpd; OrderedLocusNames=b0116, JW0112;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA   A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT   homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):547-555(1996).
RN   [2]
RP   MAPPING ON GEL.
RA   Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT   ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL   (IN) Neidhardt et al. (eds.);
RL   Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL   ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN   [3]
RP   PROTEIN EXPRESSION.
RX   MEDLINE=87194592; PubMed=3553157; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Jones P.G., Vanbogelen R.A., Neidhardt F.C.;
RT   ''''''Induction of proteins in response to low temperature in Escherichia coli'';'';'';
RL   J. Bacteriol. 169(1):2092-2095(1987).
RN   [4]
RP   PROTEIN EXPRESSION.
RX   MEDLINE=83160794; PubMed=6339477; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Smith M.W., Neidhardt F.C.;
RT   ''''''Proteins induced by aerobiosis in Escherichia coli'';'';'';
RL   J. Bacteriol. 154(1):344-350(1983).
RN   [5]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
CC   -!- SUBUNIT: HOMODIMER 
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000KPM=5.81/53704;
2D   -!-   EXPRESSION: INCREASED DURING AEROBIC GROWTH [4]; INCREASED DURING
2D         THE ADJUSTMENT OF E. COLI TO GROWTH AT 10C [3].
2D   -!-   MAPPING: GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WIU=5.82/50800;
2D   -!-   PI/MW: SPOT 2D-001WS6=5.90/28059; !FRAGMENT!
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WIU: 1011.49; 1076.62; 1139.62; 1144.64;
2D         1257.61; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WS6: 860.4; 896.44; 934.42; 1076.56;
2D         1144.56; 1333.65; 1473.76; 1571.82; 1601.86; 1713.83; 1825.98; 1971.01;
2D         2202.14; TRYPSIN.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WS6: (K)APAEPQRYDAVLVAIGR(V),256-272;
2D         (R)YDAVLVAIGR(V),263-272; (K)AGVEVDDRGFIR(V),284-295; (K)VIPSIAYTEPEVAWVGLTEK(E),345-364.
2D   -!-   MAPPING: Peptide mass fingerprinting [5]; SPOT 2D-001WS6: TANDEM
2D         MASS SPECTROMETRY [5].
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CC   ---------------------------------------------------------------------------
DR   2DBase-Ecoli; P0A9P0; DLDH_ECOLI.
DR   ECO2DBASE; G050.5; 6TH EDITION.
DR   UniProtKB/Swiss-Prot; P0A9P0; DLDH_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5


SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server