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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: ILVD_ECOLI |
SWISS-2DPAGE: ILVD_ECOLI
ILVD_ECOLI:
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ID ILVD_ECOLI; STANDARD; 2DG.
AC P05791;
DT 15-MAY-2003, integrated into SWISS-2DPAGE (release 16).
DT 15-MAY-2003, 2D annotation version 1.
DT 19-MAY-2011, general annotation version 6.
DE RecName: Full=Dihydroxy-acid dehydratase; Short=DAD; EC=4.2.1.9;.
GN Name=ilvD; OrderedLocusNames=b3771, JW5605;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
MT ECOLI-DIGE4.5-6.5.
IM ECOLI-DIGE4.5-6.5.
RN [1]
RP MAPPING ON GEL.
RX PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan]
RA Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT spectrometry based proteomic analysis of E. coli'';'';'';
RL Proteomics 2(1):1682-1698(2002).
2D -!- MASTER: ECOLI-DIGE4.5-6.5;
2D -!- PI/MW: SPOT 2D-001WFQ=5.54/62065;
2D -!- PEPTIDE MASSES: SPOT 2D-001WFQ: 858.52; 976.45; 985.53; 1018.51;
2D 1026.58; 1046.55; 1105.54; 1249.54; 1269.68; 1355.68; 1470.77; 1480.77;
2D 1484.69; 1600.88; 1626.88; 1640.77; 1693.81; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001WFQ: (K)LVAEQIEAAGGVAK(E),59-72;
2D (K)VSDSQSDQVER(S),176-186; (R)KVPQLCK(V),307-313;
2D (R)AGGVIGILGELDR(A),329-341; (K)TAGVDDSILK(F),438-447;
2D (K)TAGVDDSILKFTGPAK(V),438-453; (K)VYESQDDAVEAILGGK(V),454-469;
2D (K)ACALITDGR(F),507-515; (R)GIQLQVSDAELAARR(E),555-569.
2D -!- EXPRESSION: increase after benzoic acid treatment [1].
2D -!- MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [1].
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DR UniProtKB/Swiss-Prot; P05791; ILVD_ECOLI.
//
The following 2-D map is available for this protein:
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Escherichia coli DIGE (4.5-6.5)
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SWISS-2DPAGE (search AC)
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