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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: IMDH_ECOLI




SWISS-2DPAGE: IMDH_ECOLI



IMDH_ECOLI:

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ID   IMDH_ECOLI; STANDARD; 2DG.
AC   P0ADG7; P06981;
DT   01-AUG-1995, integrated into SWISS-2DPAGE (release 2).
DT   31-MAR-2004, 2D annotation version 4.
DT   19-MAY-2011, general annotation version 13.
DE   RecName: Full=Inosine-5'-monophosphate dehydrogenase; Short=IMP
DE   dehydrogenase; Short=IMPD; Short=IMPDH; EC=1.1.1.205;.
GN   Name=guaB; Synonyms=guaR; OrderedLocusNames=b2508, JW5401;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
MT   ECOLI, ECOLI5-6, ECOLI-DIGE4.5-6.5.
IM   ECOLI, ECOLI5-6, ECOLI-DIGE4.5-6.5.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch
RA   A., Schaller D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''Two-dimensional gel electrophoresis of Escherichia coli
RT   homogenates: the Escherichia coli SWISS-2DPAGE database'';'';'';
RL   Electrophoresis 17(1):547-555(1996).
RN   [2]
RP   MAPPING ON GEL.
RA   Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C.;
RT   ''''''Gene-protein database of Escherichia coli K-12, edition 6'';'';'';
RL   (IN) Neidhardt et al. (eds.);
RL   Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd
RL   ed.), pp.2067-2117, ASM Press, Washington DC (1996).
RN   [3]
RP   MAPPING ON GEL.
RX   MEDLINE=98410772; PubMed=9740056; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M.,
RA   Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A.,
RA   Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F.;
RT   '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'';
RL   Electrophoresis 19(1):1960-1971(1998).
RN   [4]
RP   MAPPING ON GEL.
RX   PubMed=11680886; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F.,
RA   Sanchez J.-C.;
RT   ''''''New perspectives in the Escherichia coli proteome investigation'';'';'';
RL   Proteomics 1(1):409-423(2001).
RN   [5]
RP   MAPPING ON GEL.
RX   PubMed=12469338; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S.;
RT   ''''''Fluorescence 2-D difference gel electrophoresis and mass
RT   spectrometry based proteomic analysis of E. coli'';'';'';
RL   Proteomics 2(1):1682-1698(2002).
CC   -!- SUBUNIT: HOMOTETRAMER 
2D   -!- MASTER: ECOLI;
2D   -!-   PI/MW: SPOT 2D-000KN3=5.76/56743;
2D   -!-   PI/MW: SPOT 2D-000KOE=6.01/55094;
2D   -!-   MAPPING: SPOT 2D-000KN3: AMINO ACID COMPOSITION AND SEQUENCE TAG
2D         (MLRI) [3]; SPOT 2D-000KOE: MICROSEQUENCE ANALYSIS [1] AND IDENTIFIED ON
2D         2-D GELS BY VANBOGELEN [2].
2D   -!- MASTER: ECOLI5-6;
2D   -!-   PI/MW: SPOT 2D-001LC6=5.49/38144; !FRAGMENT!
2D   -!-   PEPTIDE MASSES: SPOT 2D-001LC6: 1217.688; 1257.669; 1302.672;
2D         1786.983; 1855.026; 1986.145; 2179.223; 2335.299; 2757.502; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [4].
2D   -!- MASTER: ECOLI-DIGE4.5-6.5;
2D   -!-   PI/MW: SPOT 2D-001WH8=5.99/55298;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001WH8: 850.49; 887.43; 969.5; 1076.48;
2D         1158.48; 1217.6; 1257.58; 1302.61; 1637.93; 1786.92; 1891.99; 1986.09;
2D         2109.03; 2179.16; 2235.19; 2757.45; 3209.68; 3258.79; TRYPSIN.
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001WH8:
2D         (R)NGFAGYPVVTEENELVGIITGR(D),116-137; (R)IVTGVGVPQITAVADAVEALEGTGIPVIADGGIR(F),309-342.
2D   -!-   MAPPING: Peptide mass fingerprinting and tandem mass spectrometry [5].
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DR   UniProtKB/Swiss-Prot; P0ADG7; IMDH_ECOLI.
//


The following 2-D maps are available for this protein:
Escherichia coli
ECOLI
Escherichia coli(5-6)
ECOLI5-6
Escherichia coli DIGE (4.5-6.5)
ECOLI-DIGE4.5-6.5


SWISS-2DPAGE image

SWISS-2DPAGE (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server