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Sample Preparation and Post-separation Analysis
Searching in 'SWISS-2DPAGE' for entry matching: ODPA_STAAN |
SWISS-2DPAGE: ODPA_STAAN
ODPA_STAAN:
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ID ODPA_STAAN; STANDARD; 2DG.
AC Q820A6;
DT 31-MAR-2004, integrated into SWISS-2DPAGE (release 17).
DT 31-MAR-2004, 2D annotation version 1.
DT 19-MAY-2011, general annotation version 4.
DE RecName: Full=Pyruvate dehydrogenase E1 component subunit alpha; EC=1.2.4.1;.
GN Name=pdhA; OrderedLocusNames=SA0943.1;
OS Staphylococcus aureus (strain N315).
OC Bacteria; Firmicutes; Bacillales; Staphylococcus.
OX NCBI_TaxID=158879;
MT STAPHYLOCOCCUS.
IM STAPHYLOCOCCUS.
RN [1]
RP MAPPING ON GEL.
RX PubMed=15590099; DOI=10.1016/j.mimet.2004.09.017; [NCBI, Expasy, EBI, Israel, Japan]
RA Scherl A., Francois P., Bento M., Deshusses J.M., Charbonnier Y.,
RA Converset V., Huyghe A., Walter N., Hoogland C., Appel R.D., Sanchez
RA J.-C., Zimmermann-Ivol C.G., Corthals G.L., Hochstrasser D.F., Schrenzel J.;
RT ''''''Correlation of proteomic and transcriptomic profiles of
RT Staphylococcus aureus during the post-exponential phase of growth'';'';'';
RL J. Microbiol. Methods 60(2):247-257(2005).
CC -!- SUBUNIT: Heterodimer of an alpha and a beta chain
2D -!- MASTER: STAPHYLOCOCCUS;
2D -!- PI/MW: SPOT 2D-001XVB=4.90/49246;
2D -!- PI/MW: SPOT 2D-001XXV=4.90/38413;
2D -!- PI/MW: SPOT 2D-001XY4=4.84/37937;
2D -!- PEPTIDE MASSES: SPOT 2D-001XY4: 1019.4339; 1060.4575; 1125.4426;
2D 1337.5435; 1417.5546; 1433.4747; 1708.6639; 1724.6847; 1730.6644;
2D 1988.86; 1995.782; 1996.7836; 2416.9775; 2443.9783; 2987.0774; 3549.4209; TRYPSIN.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001XVB: (K)LQAQFDAVK(V),5-13;
2D (R)AVAGEGPTLIETMTYR(Y),254-269; (R)YGPHTMAGDDPTR(Y),270-282; (R)YGPHTMoxAGDDPTR(Y),270-282.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001XXV: (K)LQAQFDAVK(V),5-13;
2D (R)LGFYAPTAGQEASQLASQYALEKEDYILPGYR(D),74-105; (K)EDYILPGYR(D),97-105;
2D (K)APAIFVIQNNNYAISTPR(S),195-212; (R)AVAGEGPTLIETMTYR(Y),254-269;
2D (R)YGPHTMAGDDPTR(Y),270-282; (K)GLWNEDKENEVIER(A),309-322.
2D -!- PEPTIDE SEQUENCES: SPOT 2D-001XY4: (K)APAIFVIQNNNYAISTPR(S),195-212.
2D -!- MAPPING: MALDI tandem mass spectrometry [1]; SPOT 2D-001XY4:
2D Peptide mass fingerprinting [1].
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DR UniProtKB/Swiss-Prot; Q820A6; ODPA_STAAN.
//
The following 2-D map is available for this protein:
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Staphylococcus aureus
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SWISS-2DPAGE (search AC)
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