Sample Preparation and Post-separation Analysis

P04792:

ID   HSPB1_HUMAN; STANDARD; 2DG.
AC   P04792;
DT   01-APR-2000, integrated into SWISS-2DPAGE (release 12).
DT   30-DEC-2004, 2D annotation version 1.
DT   19-MAY-2011, general annotation version 8.
DE   RecName: Full=Heat shock protein beta-1; Short=HspB1; AltName: Full=28
DE   kDa heat shock protein; AltName: Full=Estrogen-regulated 24 kDa protein;
DE   AltName: Full=Heat shock 27 kDa protein; Short=HSP 27; AltName:
DE   Full=Stress-responsive protein 27; Short=SRP27;.
GN   Name=HSPB1; Synonyms=HSP27, HSP28;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   NUCLEI_LIVER_HUMAN.
IM   NUCLEI_LIVER_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=20529951; PubMed=11079567; [NCBI, Expasy, EBI, Israel, Japan]
RA   Jung E., Hoogland C., Chiappe D., Sanchez J.-C., Hochstrasser D.F.;
RT   ''''''The establishment of a human liver nuclei 2-DE reference map'';'';'';
RL   Electrophoresis 21(1):3483-3487(2000).
2D   -!- MASTER: NUCLEI_LIVER_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001JQI=5.62/24205;
2D   -!-   PI/MW: SPOT 2D-001JQP=6.18/23865;
2D   -!-   PEPTIDE MASSES: SPOT 2D-001JQI: 831.49; 917.457; 960.435; 961.454;
2D         987.612; 1104.511; 1163.626; 1783.936; 4093.564; TRYPSIN.
2D   -!-   PEPTIDE MASSES: SPOT 2D-001JQP: 831.496; 960.442; 961.456; 987.619;
2D         1075.595; 1104.529; 1163.638; 1783.926; 1906.002; TRYPSIN.
2D   -!-   MAPPING: Peptide mass fingerprinting [1].
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DR   DOSAC-COBS 2D-PAGE; P04792; P04792.
DR   DOSAC-COBS-2DPAGE; P04792; HSPB1_HUMAN.
DR   HSC-2DPAGE; P04792; HUMAN.
DR   OGP-WWW; P04792.
DR   REPRODUCTION-2DPAGE; P04792; P04792.
DR   REPRODUCTION-2DPAGE; IPI00025512; IPI00025512.
DR   Siena-2DPAGE; P04792; HSPB1_HUMAN.
DR   UCD-2DPAGE; P04792; HSPB1_HUMAN.
DR   UniProtKB/Swiss-Prot; P04792; HSPB1_HUMAN.
//

The following 2-D map is available for this protein:

	Soluble nuclear proteins and matrix from liver tissue

SWISS-2DPAGE (search AC)