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Sample Preparation and Post-separation Analysis



Searching in 'SWISS-2DPAGE' for entry matching: P09382




SWISS-2DPAGE: P09382



P09382:

Nice View - a user-friendly view of this entry
ID   LEG1_HUMAN; STANDARD; 2DG.
AC   P09382;
DT   01-SEP-1997, integrated into SWISS-2DPAGE (release 6).
DT   31-MAR-2004, 2D annotation version 3.
DT   19-MAY-2011, general annotation version 10.
DE   RecName: Full=Galectin-1; Short=Gal-1; AltName: Full=14 kDa
DE   laminin-binding protein; Short=HLBP14; AltName: Full=14 kDa lectin;
DE   AltName: Full=Beta-galactoside-binding lectin L-14-I; AltName:
DE   Full=Galaptin; AltName: Full=HBL; AltName: Full=HPL; AltName:
DE   Full=Lactose-binding lectin 1; AltName: Full=Lectin galactoside-binding
DE   soluble 1; AltName: Full=Putative MAPK-activating protein PM12; AltName:
DE   Full=S-Lac lectin 1;.
GN   Name=LGALS1;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Hominidae; Homo.
OX   NCBI_TaxID=9606;
MT   CEC_HUMAN, HL60_HUMAN, KIDNEY_HUMAN, LYMPHOCYTE_HUMAN.
IM   CEC_HUMAN, HL60_HUMAN, KIDNEY_HUMAN, LYMPHOCYTE_HUMAN.
RN   [1]
RP   MAPPING ON GEL.
RX   MEDLINE=97295305; PubMed=9150947; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Sarto C., Marocchi A., Sanchez J.-C., Giannone D., Frutiger S., Golaz O.,
RA   Wilkins M.R., Doro G., Cappellano F., Hughes G.J., Hochstrasser D.F.,
RA   Mocarelli P.;
RT   ''''''Renal cell carcinoma and normal kidney protein expression'';'';'';
RL   Electrophoresis 18(1):599-604(1997).
RN   [2]
RP   MAPPING ON GEL.
RA   Tonella L., Hoogland C., Appel R.D., Sanchez J.-C., Hochstrasser D.F.;
RL   Submitted (JUL-1997) to SWISS-2DPAGE.
RN   [3]
RP   MAPPING ON GEL.
RX   MEDLINE=96314065; PubMed=8740185; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Lutomski D., Caron M., Cornillot J.D., Bourin P., Dupuy C., Pontet M.,
RA   Bladier D., Joubert-Caron R.;
RT   ''''''Identification of different galectins by immunoblotting after two-
RT   dimensional polyacrylamide get electrophoresis with immobilized pH gradients'';'';'';
RL   Electrophoresis 17(1):600-606(1996).
RN   [4]
RP   PROTEIN EXPRESSION.
RX   MEDLINE=89093123; PubMed=2910856; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Couraud P.O., Casentini-Borocz D., Bringman T.S., Griffith J., McGrogan
RA   M., Nedwin G.E.;
RT   ''''''Molecular cloning, characterization, and expression of a human
RT   14-kDa lectin'';'';'';
RL   J. Biol. Chem. 264(1):1310-1316(1989).
RN   [5]
RP   MAPPING ON GEL.
RX   MEDLINE=98163346; PubMed=9504818; [NCBI, ExPASy, EBI, Israel, Japan]
RA   Reymond M.A., Sanchez J.-C., Hughes G.J., Riese J., Tortola S., Peinado
RA   M.A., Kirchner T., Hohenberger W., Hochstrasser D.F., Kockerling F.;
RT   ''''''Standardized characterization of gene expression in human
RT   colorectal epithelium by two-dimensional electrophoresis'';'';'';
RL   Electrophoresis 18(1):2842-2848(1997).
RN   [6]
RP   MAPPING ON GEL.
RA   Vuadens F., Crettaz D., Telenti A., Quadroni M., Duchosal M.A., Schneider
RA   P., Tissot J.-D.;
RT   ''''''New insights into HIV lymphocyte infection'';'';'';
RL   (IN) Sanchez J.-C., Corthals G.L., Hochstrasser D.F. (eds.);
RL   Biomedical Application of Proteomics, pp.245-262, Wiley-VCH, Weinheim (2004).
2D   -!- MASTER: CEC_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000UZI=4.93/12076;
2D   -!-   MAPPING: MATCHING WITH THE KIDNEY MASTER GEL [5].
2D   -!- MASTER: HL60_HUMAN;
2D   -!-   PI/MW: SPOT 2D-00104W=5.01/11094;
2D   -!-   EXPRESSION: IS UNAFFECTED BY N-GLYCANASE TREATMENT AND  IS NOT
2D         GLYCOSYLATED [4].
2D   -!-   MAPPING: COMIGRATION [2][3].
2D   -!- MASTER: KIDNEY_HUMAN;
2D   -!-   PI/MW: SPOT 2D-000O18=5.04/11822;
2D   -!-   MAPPING: MATCHING WITH THE HL60 MASTER GEL [1].
2D   -!- MASTER: LYMPHOCYTE_HUMAN;
2D   -!-   PI/MW: SPOT 2D-001YG7=4.94/16289;
2D   -!-   PEPTIDE SEQUENCES: SPOT 2D-001YG7: (R)VRGEVAPDAK(S),19-28;
2D         (K)SFVLNLGK(D),29-35; (K)DSNNLCLHFNPR(F),37-48;
2D         (R)FNAHGDANTIVCNSK(D),49-63; (K)DGGAWGTEQR(E),64-73; (K)LPDGYEFK(F),100-107.
2D   -!-   MAPPING: MALDI tandem mass spectrometry [6].
CC   ---------------------------------------------------------------------------
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CC   There are no restrictions on its use by non-profit institutions as long as
CC   its content is in no way modified and this statement is not removed.
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CC   http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss).
CC   ---------------------------------------------------------------------------
DR   DOSAC-COBS 2D-PAGE; P09382; P09382.
DR   DOSAC-COBS-2DPAGE; P09382; LEG1_HUMAN.
DR   PHCI-2DPAGE; P09382; LEG1_HUMAN.
DR   REPRODUCTION-2DPAGE; P09382; P09382.
DR   REPRODUCTION-2DPAGE; IPI00219219; IPI00219219.
DR   Siena-2DPAGE; P09382; LEG1_HUMAN.
DR   UCD-2DPAGE; P09382; LEG1_HUMAN.
DR   UniProtKB/Swiss-Prot; P09382; LEG1_HUMAN.
//


The following 2-D maps are available for this protein:
Colorectal epithelia cells
CEC_HUMAN
Promyelocytic leukemia cells
HL60_HUMAN
Kidney
KIDNEY_HUMAN
Lymphocytes
LYMPHOCYTE_HUMAN
  


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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server